| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
T Cell Responsiveness to Mycobacteria Suggestive of a Memory-Like Phenotype1
St. Louis University Vaccine Treatment and Evaluation Unit Departments of
*
Internal Medicine and
Pathology, St. Louis University Health Sciences Center, St. Louis, MO 63110
Bacille Calmette-Guérin (BCG) immunity can be studied as one
experimental model for mycobacterial protective immunity. We have used
flow cytometry to investigate human T cell subsets induced by BCG
vaccination. PBMC harvested from BCG-vaccinated individuals and
controls were stimulated with mycobacterial Ags, and the T cell subsets
present after 7 days of in vitro expansion were characterized. The most
dramatic expansions induced by mycobacterial Ags were detected in
T cells. The T cell expansions measured after in vitro
stimulation with mycobacterial Ags were significantly greater in BCG
responders compared with nonsensitized controls, indicating that BCG
vaccination induced T cell activation associated with enhanced
secondary responses. The majority of T cells induced by BCG
vaccination were 9+2+ T
cells reactive with isoprenyl pyrophosphates. Coculture with
CD4+ T cells induced optimal T cell expansion,
although IL-2 alone could provide this helper function in the absence
of CD4+ T cells. T cells were found to provide
helper functions for mycobacterial specific CD4+ and
CD8+ T cells as well, demonstrating reciprocal stimulatory
interactions between T cells and other T cell subsets. Finally,
prominent mycobacterial specific T cell expansions were detected
in a subset of unvaccinated controls with evidence for prior
sensitization to mycobacterial lysates (elevated mycobacterial specific
lymphoproliferative responses). These latter findings are consistent
with the hypothesis that exposure to atypical mycobacteria or related
environmental Ags may induce T cells cross-reactive with Ags
present in the Mycobacterium tuberculosis complex. Our
results suggest that T cells may be capable of developing a
memory immune-like phenotype, and therefore might be important targets
for new vaccines.
This article has been cited by other articles:
|
|
 |
|
  C. T. Spencer, G. Abate, A. Blazevic, and D. F. Hoft Only a Subset of Phosphoantigen-Responsive {gamma}9{delta}2 T Cells Mediate Protective Tuberculosis Immunity J. Immunol., October 1, 2008; 181(7): 4471 - 4484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Pitard, D. Roumanes, X. Lafarge, L. Couzi, I. Garrigue, M.-E. Lafon, P. Merville, J.-F. Moreau, and J. Dechanet-Merville Long-term expansion of effector/memory V{delta}2- {gamma}{delta} T cells is a specific blood signature of CMV infection Blood, August 15, 2008; 112(4): 1317 - 1324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Li and C.-Y. Wu CD4+CD25+ Treg cells inhibit human memory {gamma}{delta} T cells to produce IFN-{gamma} in response to M tuberculosis antigen ESAT-6 Blood, June 15, 2008; 111(12): 5629 - 5636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Martino, R. Casetti, A. Sacchi, and F. Poccia Central Memory V{gamma}9V{delta}2 T Lymphocytes Primed and Expanded by Bacillus Calmette-Guerin-Infected Dendritic Cells Kill Mycobacterial-Infected Monocytes J. Immunol., September 1, 2007; 179(5): 3057 - 3064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lockhart, A. M. Green, and J. L. Flynn IL-17 Production Is Dominated by {gamma}{delta} T Cells rather than CD4 T Cells during Mycobacterium tuberculosis Infection J. Immunol., October 1, 2006; 177(7): 4662 - 4669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Casetti, G. Perretta, A. Taglioni, M. Mattei, V. Colizzi, F. Dieli, G. D'Offizi, M. Malkovsky, and F. Poccia Drug-Induced Expansion and Differentiation of V{gamma}9V{delta}2 T Cells In Vivo: The Role of Exogenous IL-2 J. Immunol., August 1, 2005; 175(3): 1593 - 1598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Nonnecke, W. R. Waters, M. R. Foote, M. V. Palmer, B. L. Miller, T. E. Johnson, H. B. Perry, and M. A. Fowler Development of an Adult-Like Cell-Mediated Immune Response in Calves After Early Vaccination with Mycobacterium bovis bacillus Calmette-Guerin J Dairy Sci, January 1, 2005; 88(1): 195 - 210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lee, K. Choi, M. R. Olin, S.-N. Cho, and T. W. Molitor {gamma}{delta} T Cells in Immunity Induced by Mycobacterium bovis Bacillus Calmette-Guerin Vaccination Infect. Immun., March 1, 2004; 72(3): 1504 - 1511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Dieli, F. Poccia, M. Lipp, G. Sireci, N. Caccamo, C. Di Sano, and A. Salerno Differentiation of Effector/Memory V{delta}2 T Cells and Migratory Routes in Lymph Nodes or Inflammatory Sites J. Exp. Med., August 4, 2003; 198(3): 391 - 397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Klimpel, M. A. Matthias, and J. M. Vinetz Leptospira interrogans Activation of Human Peripheral Blood Mononuclear Cells: Preferential Expansion of TCR{gamma}{delta}+ T Cells vs TCR{alpha}{beta}+ T Cells J. Immunol., August 1, 2003; 171(3): 1447 - 1455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Worku and D. F. Hoft Differential Effects of Control and Antigen-Specific T Cells on Intracellular Mycobacterial Growth Infect. Immun., April 1, 2003; 71(4): 1763 - 1773. [Abstract] [Full Text]
|
|
|
|
|
|
R. E. Rojas, M. Torres, J.-J. Fournie, C. V. Harding, and W. H. Boom Phosphoantigen Presentation by Macrophages to Mycobacterium tuberculosis-Reactive V{gamma}9V{delta}2+ T Cells: Modulation by Chloroquine Infect. Immun., August 1, 2002; 70(8): 4019 - 4027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gioia, C. Agrati, R. Casetti, C. Cairo, G. Borsellino, L. Battistini, G. Mancino, D. Goletti, V. Colizzi, L. P. Pucillo, et al. Lack of CD27-CD45RA-V{gamma}9V{delta}2+ T Cell Effectors in Immunocompromised Hosts and During Active Pulmonary Tuberculosis J. Immunol., February 1, 2002; 168(3): 1484 - 1489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Giuliani, S. P. Prete, G. Graziani, A. Aquino, A. Balduzzi, M. Sugita, M. B. Brenner, E. Iona, L. Fattorini, G. Orefici, et al. Influence of Mycobacterium bovis Bacillus Calmette Guerin on In Vitro Induction of CD1 Molecules in Human Adherent Mononuclear Cells Infect. Immun., December 1, 2001; 69(12): 7461 - 7470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fjallbrant, M. Ridell, and L.O. Larsson The tuberculin skin test in relation to immunological in vitro reactions in BCG-vaccinated healthcare workers Eur. Respir. J., August 1, 2001; 18(2): 376 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Morita, H. K. Lee, H. Wang, H. Li, R. A. Mariuzza, and Y. Tanaka Structural Features of Nonpeptide Prenyl Pyrophosphates That Determine Their Antigenicity for Human {{gamma}}{{delta}} T Cells J. Immunol., July 1, 2001; 167(1): 36 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Rhodes, R. G. Hewinson, and H. M. Vordermeier Antigen Recognition and Immunomodulation by {{gamma}}{{delta}} T Cells in Bovine Tuberculosis J. Immunol., May 1, 2001; 166(9): 5604 - 5610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Rojas, K. N. Balaji, A. Subramanian, and W. H. Boom Regulation of Human CD4+ alpha beta T-Cell-Receptor-Positive (TCR+) and gamma delta TCR+ T-Cell Responses to Mycobacterium tuberculosis by Interleukin-10 and Transforming Growth Factor beta Infect. Immun., December 1, 1999; 67(12): 6461 - 6472. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
