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Departments of
*
Pediatrics and
Biochemistry, Gifu University School of Medicine, Gifu, Japan
Apoptosis is an active form of cell death that can be induced by a
wide variety of agents and conditions. In response to actinomycin D,
hydrogen peroxide (H2O2), or TNF-
, Jurkat T
cells underwent typical apoptosis. Phospholipase D (PLD) activity in
intact cells determined by phosphatidylbutanol generation was
up-regulated by these agents. The PLD activation was in a
time-dependent manner during apoptosis. It was also shown that the PLD
activity measured by using exogenous substrate in the lysate from
apoptotic cells was higher than that in the lysate from control
untreated cells. The PLD activity in lysate from control untreated
cells was stimulated by unsaturated fatty acids (UFA), but not by
guanosine 5'-O-(3-thiotriphosphate). However, the PLD
activity in the apoptotic cell lysate was no longer enhanced by the
addition of oleate, suggesting that the increased PLD activity during
apoptosis was attributed to the PLD of UFA-dependent type, but not the
small G protein-dependent one. In fact, the release of free UFA was
increased during apoptosis. The caspase inhibitors, z-DEVD and z-VAD,
effectively suppressed PLD activation and apoptosis, but UFA release
was unaffected. These results suggest the possibility that
UFA-dependent type PLD may be implicated in apoptotic process in Jurkat
T cells. This is the first demonstration that the PLD of UFA-dependent
type would be involved in cellular responses.
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