| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
(1,3)-Fucosyltransferase VII-Dependent Synthesis of P- and E-Selectin Ligands on Cultured T Lymphoblasts1
*
Department of Pathology and
Howard Hughes Medical Research Institute, University of Michigan, Ann Arbor, MI 48109
T lymphocytes up-regulate the synthesis of ligands for E- and
P-selectin during proliferative responses in vivo and in vitro.
Previous studies from our laboratories indicated that the
(1,3)-fucosyltransferase FucT-VII regulates the synthesis of
E-selectin ligands and sialylated Lewisx-related epitopes
(sLex-related epitopes) in human T lymphoblasts. The
current report shows that production of both P- and E-selectin ligands
is FucT-VII dependent, but peak synthesis of each occurs at different
levels of fucosyltransferase activity in intact cells. In brief,
FucT-VII mRNA levels were higher in cultured T lymphoblasts expressing
sLex-related epitopes and both selectin ligands than in
cells expressing P-selectin ligands alone. However, synthesis of the
epitopes and both selectin ligands required the FucT-VII enzyme in
transfected Molt-4 cells. In contrast, neither constitutive nor
transfection-enhanced levels of the FucT-IV enzyme generated active
P-selectin ligands in these lines. In addition, targeted deletion of
the FucT-VII gene in mice markedly inhibited the synthesis of both P-
and E-selectin ligands during blast transformation in vitro. Finally,
the optimal synthesis of active P-selectin ligands occurred at lower
level of FucT-VII activity than required for synthesis of equally
active E-selectin ligands in both cultured T lymphoblasts and FucT-VII
transfectants. Consequently, the FucT-VII enzyme is essential for the
synthesis of both P- and E-selectin ligands by T lymphoblasts, and its
activity determines whether P-selectin ligands are expressed alone or
in conjunction with E-selectin ligands and sLex-related
epitopes on human T cells.
This article has been cited by other articles:
|
|
 |
|
  S. R Barthel, J. D Gavino, G. K Wiese, J. M Jaynes, J. Siddiqui, and C. J Dimitroff Analysis of glycosyltransferase expression in metastatic prostate cancer cells capable of rolling activity on microvascular endothelial (E)-selectin Glycobiology, October 1, 2008; 18(10): 806 - 817. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. An, H. Wang, R. Tang, T. Yago, J. M. McDaniel, S. McGee, Y. Huo, and L. Xia P-Selectin Glycoprotein Ligand-1 Is Highly Expressed on Ly-6Chi Monocytes and a Major Determinant for Ly-6Chi Monocyte Recruitment to Sites of Atherosclerosis in Mice Circulation, June 24, 2008; 117(25): 3227 - 3237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Syrbe, U. Hoffmann, K. Schlawe, O. Liesenfeld, K. Erb, and A. Hamann Microenvironment-Dependent Requirement of STAT4 for the Induction of P-Selectin Ligands and Effector Cytokines on CD4+ T Cells in Healthy and Parasite-Infected Mice J. Immunol., December 1, 2006; 177(11): 7673 - 7679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Ni, J. J. Campbell, G. Niehans, and B. Walcheck The Monoclonal Antibody CHO-131 Identifies a Subset of Cutaneous Lymphocyte-Associated Antigen T Cells Enriched in P-Selectin-Binding Cells J. Immunol., October 1, 2006; 177(7): 4742 - 4748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. H. Cai, A. Alvarez, P. Alcaide, P. Duramad, Y.-C. Lim, P. Jarolim, J. B. Lowe, F. W. Luscinskas, and A. H. Lichtman Abrogation of Functional Selectin-Ligand Expression Reduces Migration of Pathogenic CD8+ T Cells into Heart. J. Immunol., June 1, 2006; 176(11): 6568 - 6575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Bonder, M. U. Norman, T. MacRae, P. R. Mangan, C. T. Weaver, D. C. Bullard, D.-M. McCafferty, and P. Kubes P-Selectin Can Support Both Th1 and Th2 Lymphocyte Rolling in the Intestinal Microvasculature Am. J. Pathol., December 1, 2005; 167(6): 1647 - 1660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Mangan, D. O'Quinn, L. Harrington, C. S. Bonder, P. Kubes, D. F. Kucik, D. C. Bullard, and C. T. Weaver Both Th1 and Th2 Cells Require P-Selectin Glycoprotein Ligand-1 for Optimal Rolling on Inflamed Endothelium Am. J. Pathol., December 1, 2005; 167(6): 1661 - 1675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. H. Underhill, D. G. Zisoulis, K. P. Kolli, L. G. Ellies, J. D. Marth, and G. S. Kansas A crucial role for T-bet in selectin ligand expression in T helper 1 (Th1) cells Blood, December 1, 2005; 106(12): 3867 - 3873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Siegmund, M. Feuerer, C. Siewert, S. Ghani, U. Haubold, A. Dankof, V. Krenn, M. P. Schon, A. Scheffold, J. B. Lowe, et al. Migration matters: regulatory T-cell compartmentalization determines suppressive activity in vivo Blood, November 1, 2005; 106(9): 3097 - 3104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Satoh, Y. Kanai, M.-H. Wu, H. Yokozeki, R. Kannagi, J. B. Lowe, and K. Nishioka Synthesis of {alpha}(1,3) Fucosyltransferases IV- and VII-Dependent Eosinophil Selectin Ligand and Recruitment to the Skin Am. J. Pathol., September 1, 2005; 167(3): 787 - 796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yoo, M. Omori, D. Gyarmati, B. Zhou, T. Aye, A. Brewer, M. R. Comeau, D. J. Campbell, and S. F. Ziegler Spontaneous atopic dermatitis in mice expressing an inducible thymic stromal lymphopoietin transgene specifically in the skin J. Exp. Med., August 15, 2005; 202(4): 541 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zou, V. R. Shinde Patil, N. M. Dagia, L. A. Smith, M. J. Wargo, K. A. Interliggi, C. M. Lloyd, D. F. J. Tees, B. Walcheck, M. B. Lawrence, et al. PSGL-1 derived from human neutrophils is a high-efficiency ligand for endothelium-expressed E-selectin under flow Am J Physiol Cell Physiol, August 1, 2005; 289(2): C415 - C424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Martinez, M. Joffraud, S. Giraud, B. Baisse, M. P. Bernimoulin, M. Schapira, and O. Spertini Regulation of PSGL-1 Interactions with L-selectin, P-selectin, and E-selectin: ROLE OF HUMAN FUCOSYLTRANSFERASE-IV AND -VII J. Biol. Chem., February 18, 2005; 280(7): 5378 - 5390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Syrbe, S. Jennrich, A. Schottelius, A. Richter, A. Radbruch, and A. Hamann Differential regulation of P-selectin ligand expression in naive versus memory CD4+ T cells: evidence for epigenetic regulation of involved glycosyltransferase genes Blood, November 15, 2004; 104(10): 3243 - 3248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Zisoulis and G. S. Kansas H-Ras and Phosphoinositide 3-Kinase Cooperate to Induce {alpha}(1,3)-Fucosyltransferase VII Expression in Jurkat T Cells J. Biol. Chem., September 17, 2004; 279(38): 39495 - 39504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Takahashi, Y. Mizukawa, Y. Yamazaki, K. Hayakawa, J. Hayakawa, A. Kudo, and T. Shiohara In Vitro Differentiation from Naive to Mature E-Selectin Binding CD4 T Cells: Acquisition of Skin-Homing Properties Occurs Independently of Cutaneous Lymphocyte Antigen Expression J. Immunol., December 1, 2003; 171(11): 5769 - 5777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zaph and P. Scott Th1 Cell-Mediated Resistance to Cutaneous Infection with Leishmania major Is Independent of P- and E-Selectins J. Immunol., November 1, 2003; 171(9): 4726 - 4732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Barry, D. G. Zisoulis, J. W. Neal, N. A. Clipstone, and G. S. Kansas Induction of FucT-VII by the Ras/MAP kinase cascade in Jurkat T cells Blood, September 1, 2003; 102(5): 1771 - 1778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hiraiwa, T. Yabuta, K. Yoritomi, M. Hiraiwa, Y. Tanaka, T. Suzuki, M. Yoshida, and R. Kannagi Transactivation of the fucosyltransferase VII gene by human T-cell leukemia virus type 1 Tax through a variant cAMP-responsive element Blood, May 1, 2003; 101(9): 3615 - 3621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Stephens and D. D. Chaplin IgE Cross-Linking or Lipopolysaccharide Treatment Induces Recruitment of Th2 Cells to the Lung in the Absence of Specific Antigen J. Immunol., November 15, 2002; 169(10): 5468 - 5476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Rao, D. O. Haskard, and R. C. Landis Enhanced Recruitment of Th2 and CLA-Negative Lymphocytes by the S128R Polymorphism of E-Selectin J. Immunol., November 15, 2002; 169(10): 5860 - 5865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Jutila, S. Kurk, L. Jackiw, R. N. Knibbs, and L. M. Stoolman L-Selectin Serves as an E-Selectin Ligand on Cultured Human T Lymphoblasts J. Immunol., August 15, 2002; 169(4): 1768 - 1773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Fuhlbrigge, S. L. King, C. J. Dimitroff, T. S. Kupper, and R. Sackstein Direct Real-Time Observation of E- and P-Selectin-Mediated Rolling on Cutaneous Lymphocyte-Associated Antigen Immobilized on Western Blots J. Immunol., June 1, 2002; 168(11): 5645 - 5651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Walcheck, A. Leppanen, R. D. Cummings, R. N. Knibbs, L. M. Stoolman, S. R. Alexander, P. E. Mattila, and R. P. McEver The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated with sialyl-Lewis x, which is a functional glycan ligand for P-selectin Blood, May 13, 2002; 99(11): 4063 - 4069. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lacha, A. Bushell, K. Smetana, P. Rossmann, P. Pribylova, K. Wood, and P. Maly Intercellular cell adhesion molecule-1 and selectin ligands in acute cardiac allograft rejection: a study on gene-deficient mouse models J. Leukoc. Biol., February 1, 2002; 71(2): 311 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Campbell and E. C. Butcher Rapid Acquisition of Tissue-specific Homing Phenotypes by CD4+ T Cells Activated in Cutaneous or Mucosal Lymphoid Tissues J. Exp. Med., January 7, 2002; 195(1): 135 - 141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Reiss, A. E. Proudfoot, C. A. Power, J. J. Campbell, and E. C. Butcher CC Chemokine Receptor (CCR)4 and the CCR10 Ligand Cutaneous T Cell-attracting Chemokine (CTACK) in Lymphocyte Trafficking to Inflamed Skin J. Exp. Med., November 19, 2001; 194(10): 1541 - 1547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Lim, H. Xie, C. E. Come, S. I. Alexander, M. J. Grusby, A. H. Lichtman, and F. W. Luscinskas IL-12, STAT4-Dependent Up-Regulation of CD4+ T Cell Core 2 {beta}-1,6-n-Acetylglucosaminyltransferase, an Enzyme Essential for Biosynthesis of P-Selectin Ligands J. Immunol., October 15, 2001; 167(8): 4476 - 4484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tanigawa, N. Takeshita, R. A. Craig, K. Phillips, R. N. Knibbs, A. E. Chang, and L. M. Stoolman Tumor-Specific Responses in Lymph Nodes Draining Murine Sarcomas Are Concentrated in Cells Expressing P-Selectin Binding Sites J. Immunol., September 15, 2001; 167(6): 3089 - 3098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Snapp, C. E. Heitzig, L. G. Ellies, J. D. Marth, and G. S. Kansas Differential requirements for the O-linked branching enzyme core 2 {beta}1-6-N-glucosaminyltransferase in biosynthesis of ligands for E-selectin and P-selectin Blood, June 15, 2001; 97(12): 3806 - 3811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Naiyer, D.-Y. Jo, J. Ahn, R. Mohle, M. Peichev, G. Lam, R. L. Silverstein, M. A.S. Moore, and S. Rafii Stromal Derived Factor-1-Induced Chemokinesis of Cord Blood CD34+ Cells (Long-Term Culture-Initiating Cells) Through Endothelial Cells Is Mediated by E-Selectin Blood, December 15, 1999; 94(12): 4011 - 4019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Blander, I. Visintin, C. A. Janeway Jr., and R. Medzhitov {alpha}(1,3)-Fucosyltransferase VII and {alpha}(2,3)-Sialyltransferase IV Are Up-Regulated in Activated CD4 T Cells and Maintained After Their Differentiation into Th1 and Migration into Inflammatory Sites J. Immunol., October 1, 1999; 163(7): 3746 - 3752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xie, Y.-C. Lim, F. W. Luscinskas, and A. H. Lichtman Acquisition of Selectin Binding and Peripheral Homing Properties by CD4+ and CD8+ T Cells J. Exp. Med., June 7, 1999; 189(11): 1765 - 1776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Lim, L. Henault, A. J. Wagers, G. S. Kansas, F. W. Luscinskas, and A. H. Lichtman Expression of Functional Selectin Ligands on Th Cells Is Differentially Regulated by IL-12 and IL-4 J. Immunol., March 15, 1999; 162(6): 3193 - 3201. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
