HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fujisawa, N. Articles by Miller, E. J. Search for Related Content PubMed PubMed Citation Articles by Fujisawa, N. Articles by Miller, E. J.

Staphylococcal Enterotoxin A-Induced Injury of Human Lung Endothelial Cells and IL-8 Accumulation Are Mediated by TNF-¹

Nobumitsu Fujisawa^*, Shinichiro Hayashi, Anna Kurdowska^*, James M. Noble^*, Keiko Naitoh and Edmund J. Miller^2,*

^* Department of Biochemistry, University of Texas Health Center, Tyler, TX 75710; and Department of Medicine, Saga Medical School, Saga, Japan

Staphylococcal enterotoxin A (SEA), a superantigen produced by some strains of Staphylococcus aureus, causes a variety of clinical manifestations ranging from food poisoning to shock. S. aureus can also be associated with the development of acute respiratory distress syndrome, and SEA has been shown to cause an inflammatory reaction in the lung. Therefore, we examined possible interactions between SEA, PBMCs, polymorphonuclear cells (PMNs), and normal human lung microvascular endothelial cells (HMVEC-L), as well as the role of these interactions on the secretion of IL-8. Injury to HMVEC-L, as measured by the release of ⁵¹Cr, increased significantly when HMVEC-L were incubated with SEA and PBMCs. IL-8 was secreted by both PBMCs and HMVEC-L. The accumulation of IL-8 in the culture medium of HMVEC-L was increased by SEA in a dose-dependent manner and was directly related to the number of PBMCs present. Although neither anti-human IL-8 nor IL-1 mAb inhibited HMVEC-L cytotoxicity, anti-human TNF- mAb inhibited both the cytotoxicity and IL-8 accumulation completely. When HMVEC-L were incubated with supernatants from SEA-treated PBMCs, HMVEC-L cytotoxicity was comparable with HMVEC-L incubated with SEA and PBMCs at the same time. Although high concentrations of purified PMNs induced HMVEC-L lysis in a dose-dependent manner, the effect of PMNs was not changed in the presence of SEA. These findings suggest that TNF- secreted by SEA-stimulated PBMCs plays a leading role in HMVEC-L injury.

This article has been cited by other articles:

				S. Ren, D. S. Terman, G. Bohach, A. Silvers, C. Hansen, H. Colt, and S. A. Sahn Intrapleural Staphylococcal Superantigen Induces Resolution of Malignant Pleural Effusions and a Survival Benefit in Non-Small Cell Lung Cancer Chest, November 1, 2004; 126(5): 1529 - 1539. [Abstract] [Full Text] [PDF]

				M. L. Hale, S. B. Margolin, T. Krakauer, C. J. Roy, and B. G. Stiles Pirfenidone Blocks the In Vitro and In Vivo Effects of Staphylococcal Enterotoxin B Infect. Immun., June 1, 2002; 70(6): 2989 - 2994. [Abstract] [Full Text] [PDF]

				J. Ben-Ari, D. Gozal, R. J. Dorio, C. M. Bowman, A. Reiff, and S. M. Walker Superantigens and Cystic Fibrosis: Resistance of Presenting Cells to Dexamethasone Clin. Vaccine Immunol., July 1, 2000; 7(4): 553 - 556. [Abstract] [Full Text] [PDF]