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Laboratory of Microbiology and Immunology of Infection, Institute for Molecular and Cell Biology, University of Porto, Porto, Portugal
IL-12 is a pivotal cytokine in the induction of IFN-
-mediated
protective immune responses. We tested the effects of rIL-12
administration to Mycobacterium avium-infected mice and
found a limited ability to induce protection against the infection;
this ability varied according to the mycobacterial strain
studied. IL-12 accelerated the expression and production of
IFN- in both immunocompetent and immunodeficient SCID or
CD4-depleted mice. Evidence of NK cell activation was found as well as
an enhancement of the ability to adoptively transfer resistance with T
cell-enriched spleen cell populations and an increase in inflammatory
cell recruitment in the liver. The protective ability of IL-12 was
dependent upon the endogenous production of IFN- as evaluated by the
use of specific neutralizing Abs or IFN- gene-disrupted mice. IL-12
potentiated the protective immunity conferred by a subunit vaccine
containing M. avium culture filtrate proteins and
dimethyl dioctadecyl ammonium chloride as an adjuvant. Thus, we show
limited immunotherapeutic benefits from IL-12 administration in
M. avium infections and promising results in its use as
a coadjuvant in vaccine design.
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