HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bonorino, C. Articles by Wysocki, L. J. Search for Related Content PubMed PubMed Citation Articles by Bonorino, C. Articles by Wysocki, L. J. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Substance via MeSH

Characteristics of the Strong Antibody Response to Mycobacterial Hsp70: A Primary, T Cell-Dependent IgG Response with no Evidence of Natural Priming or T Cell Involvement¹

Cristina Bonorino^2,*, Nance B. Nardi^*, Xianghua Zhang^, and Lawrence J. Wysocki^,

^* Department of Microbiology Pontificia Universidade Catolica do Rio Grande do SulAv, Porto Alegre, Brazil; Department of Pediatrics, Division of Basic Sciences, National Jewish Medical and Research Center, Denver, CO 80206; and Department of Immunology, University of Colorado Health Sciences Center, Denver, CO 80262

Despite its high degree of evolutionary conservation, hsp70 is a surprisingly robust Ag, to such a degree that it is under consideration as a potential substrate in vaccine development. The cellular basis of the strong humoral response, however, is unknown, although it is often hypothesized to derive from restimulation of memory T cells that have been primed by hsp of intestinal flora. In this study, we tested this hypothesis and performed additional studies on the immune response to hsp70 of Mycobacterium tuberculosis. Superficially, the primary Ab response to this protein resembles a T cell-dependent secondary one, constituted almost exclusively by IgG. However, there is no evidence of natural priming, as revealed both by in vitro stimulation experiments and by immunity in germfree mice. Although hsp70 stimulates and ß T cells from unprimed mice to proliferate in vitro, cells are not required for the strong humoral response, which is indistinguishable in normal and T cell-deficient mice. Thus, the unusual immunogenicity of this protein in eliciting a humoral response appears to be due to a strong ß T cell response with no evidence of natural priming or a T cell involvement.

This article has been cited by other articles:

				R. Duarte, A. M. Silva, L. Q. Vieira, L. C. C. Afonso, and J. R. Nicoli Influence of normal microbiota on some aspects of the immune response during experimental infection with Trypanosoma cruzi in mice J. Med. Microbiol., August 1, 2004; 53(8): 741 - 748. [Abstract] [Full Text] [PDF]

				C. Maranon, L. Planelles, C. Alonso, and M. C. Lopez HSP70 from Trypanosoma cruzi is endowed with specific cell proliferation potential leading to apoptosis Int. Immunol., December 1, 2000; 12(12): 1685 - 1693. [Abstract] [Full Text] [PDF]

				V. Julia, S. S. McSorley, L. Malherbe, J.-P. Breittmayer, F. Girard-Pipau, A. Beck, and N. Glaichenhaus Priming by Microbial Antigens from the Intestinal Flora Determines the Ability of CD4+ T Cells to Rapidly Secrete IL-4 in BALB/c Mice Infected with Leishmania major J. Immunol., November 15, 2000; 165(10): 5637 - 5645. [Abstract] [Full Text] [PDF]

				S. Tanaka, S. Itohara, M. Sato, T. Taniguchi, and Y. Yokomizo Reduced Formation of Granulomata in {gamma}{delta} T Cell Knockout BALB/c Mice Inoculated with Mycobacterium avium subsp. paratuberculosis Vet. Pathol., September 1, 2000; 37(5): 415 - 421. [Abstract] [Full Text] [PDF]