| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,§
,§
Departments of
*
Molecular Immunology,
Medicine, and
Flow Cytometry, Roswell Park Cancer Institute, Buffalo, NY 14263; and
§
Department of Hematology/Oncology, Arthur G. James Cancer Hospital and Research Institute, Ohio State University, Columbus, OH 43210
NK cells are the first line of defense against foreign cells,
virally infected cells, and tumors. The mechanisms whereby NK cells
accumulate in extralymphoid sites in response to pathogenic stimuli are
not well understood. The L-selectin adhesion molecule (CD62L) plays a
primary role in mediating the initial interaction of leukocytes with
vascular endothelium, a crucial step in the extravasation of immune
effector cells into tissues. In this report, we show L-selectin to be
uniquely expressed on a subset of resting human NK cells
(CD56bright). Notably, CD56bright NK
cells expressed L-selectin at a higher density than all other
peripheral blood leukocytes. NK activation by PMA, IL-2, IL-15, or
TGF-ß down-regulated L-selectin on the CD56bright subset,
while increased L-selectin levels were observed in both the
CD56bright and CD56dim NK subsets in response
to IL-12, IL-10, or IFN-
. Moreover, CD56bright NK cells
bound with high efficiency to physiologic L-selectin ligands on
peripheral lymph node high endothelial venules (HEV). In sharp
contrast, CD56dim NK cells adhered poorly to HEV and were
predominantly L-selectin- or expressed L-selectin only at
low density. In CD56bright cells and a subpopulation of
CD56dim cells, L-selectin ligation by mAb cross-linking
activated lymphocyte function-associated Ag 1 (LFA-1), a second
adhesion molecule required for leukocyte extravasation. LFA-1 was
expressed on both NK subsets, although its density was constitutively
higher on CD56dim cells. Taken together, evidence of
differential expression of L-selectin and LFA-1 on
CD56bright and CD56dim NK subsets strongly
suggests unique migratory properties and functions of these cells
during the early immune response to foreign pathogens.
This article has been cited by other articles:
|
|
 |
|
  O. Sobolev, P. Stern, A. Lacy-Hulbert, and R. O. Hynes Natural Killer Cells Require Selectins for Suppression of Subcutaneous Tumors Cancer Res., March 15, 2009; 69(6): 2531 - 2539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kitaya and T. Yasuo Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium J. Leukoc. Biol., March 1, 2009; 85(3): 391 - 400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Silva, D. M. Andrews, A. G. Brooks, M. J. Smyth, and Y. Hayakawa Application of CD27 as a marker for distinguishing human NK cell subsets Int. Immunol., April 1, 2008; 20(4): 625 - 630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Schierloh, N. Yokobori, M. Aleman, V. Landoni, L. Geffner, R. M. Musella, J. Castagnino, M. Baldini, E. Abbate, S. S. de la Barrera, et al. Mycobacterium tuberculosis-Induced Gamma Interferon Production by Natural Killer Cells Requires Cross Talk with Antigen-Presenting Cells Involving Toll-Like Receptors 2 and 4 and the Mannose Receptor in Tuberculous Pleurisy Infect. Immun., November 1, 2007; 75(11): 5325 - 5337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Harlin, M. Hanson, C. C. Johansson, D. Sakurai, I. Poschke, H. Norell, K.-J. Malmberg, and R. Kiessling The CD16 CD56bright NK Cell Subset Is Resistant to Reactive Oxygen Species Produced by Activated Granulocytes and Has Higher Antioxidative Capacity Than the CD16+CD56dim Subset J. Immunol., October 1, 2007; 179(7): 4513 - 4519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. B. Thoren, A. I. Romero, S. Hermodsson, and K. Hellstrand The CD16-/CD56bright Subset of NK Cells Is Resistant to Oxidant-Induced Cell Death J. Immunol., July 15, 2007; 179(2): 781 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Chan, D.-L. Hong, A. Atzberger, S. Kollnberger, A. D. Filer, C. D. Buckley, A. McMichael, T. Enver, and P. Bowness CD56bright Human NK Cells Differentiate into CD56dim Cells: Role of Contact with Peripheral Fibroblasts J. Immunol., July 1, 2007; 179(1): 89 - 94. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Ramos, G. C. Fernandez, N. Patey, P. Schierloh, R. Exeni, I. Grimoldi, G. Vallejo, C. Elias-Costa, M. del Carmen Sasiain, H. Trachtman, et al. Involvement of the fractalkine pathway in the pathogenesis of childhood hemolytic uremic syndrome Blood, March 15, 2007; 109(6): 2438 - 2445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Freud, A. Yokohama, B. Becknell, M. T. Lee, H. C. Mao, A. K. Ferketich, and M. A. Caligiuri Evidence for discrete stages of human natural killer cell differentiation in vivo J. Exp. Med., April 17, 2006; 203(4): 1033 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hayakawa and M. J. Smyth CD27 Dissects Mature NK Cells into Two Subsets with Distinct Responsiveness and Migratory Capacity J. Immunol., February 1, 2006; 176(3): 1517 - 1524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Endsley, M. A. Endsley, and D. M. Estes Bovine natural killer cells acquire cytotoxic/effector activity following activation with IL-12/15 and reduce Mycobacterium bovis BCG in infected macrophages J. Leukoc. Biol., January 1, 2006; 79(1): 71 - 79. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yamaguchi, K. Kitaya, N. Daikoku, T. Yasuo, S. Fushiki, and H. Honjo Potential Selectin L Ligands Involved in Selective Recruitment of Peripheral Blood CD16(-) Natural Killer Cells into Human Endometrium Biol Reprod, January 1, 2006; 74(1): 35 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chen, H. Kawashima, J. B. Lowe, L. L. Lanier, and M. Fukuda Suppression of tumor formation in lymph nodes by L-selectin-mediated natural killer cell recruitment J. Exp. Med., December 19, 2005; 202(12): 1679 - 1689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Schierloh, N. Yokobori, M. Aleman, R. M. Musella, M. Beigier-Bompadre, M. A. Saab, L. Alves, E. Abbate, S. S. de la Barrera, and M. C. Sasiain Increased Susceptibility to Apoptosis of CD56dimCD16+ NK Cells Induces the Enrichment of IFN-{gamma}-Producing CD56bright Cells in Tuberculous Pleurisy J. Immunol., November 15, 2005; 175(10): 6852 - 6860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wu, L.-P. Jin, M.-M. Yuan, Y. Zhu, M.-Y. Wang, and D.-J. Li Human First-Trimester Trophoblast Cells Recruit CD56brightCD16- NK Cells into Decidua by Way of Expressing and Secreting of CXCL12/Stromal Cell-Derived Factor 1 J. Immunol., July 1, 2005; 175(1): 61 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. van den Heuvel, J. Horrocks, S. Bashar, K. Hatta, S. Burke, S. S. Evans, B. A. Croy, and F. R. Tekpetey Periovulatory Increases in Tissue Homing Potential of Circulating CD56bright Cells Are Associated with Fertile Menstrual Cycles J. Clin. Endocrinol. Metab., June 1, 2005; 90(6): 3606 - 3613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nguyen, N. Dhedin, J.-P. Vernant, M. Kuentz, A. A. Jijakli, N. Rouas-Freiss, E. D. Carosella, A. Boudifa, P. Debre, and V. Vieillard NK-cell reconstitution after haploidentical hematopoietic stem-cell transplantations: immaturity of NK cells and inhibitory effect of NKG2A override GvL effect Blood, May 15, 2005; 105(10): 4135 - 4142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Dyugovskaya, P. Lavie, M. Hirsh, and L. Lavie Activated CD8+ T-lymphocytes in obstructive sleep apnoea Eur. Respir. J., May 1, 2005; 25(5): 820 - 828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Bosch, G. G. Berntson, J. T. Cacioppo, and P. T. Marucha Differential Mobilization of Functionally Distinct Natural Killer Subsets During Acute Psychologic Stress Psychosom Med, May 1, 2005; 67(3): 366 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. van den Heuvel, J. Horrocks, S. Bashar, S. Taylor, S. Burke, K. Hatta, J. E. Lewis, and B. A. Croy Menstrual Cycle Hormones Induce Changes in Functional Interactions between Lymphocytes and Decidual Vascular Endothelial Cells J. Clin. Endocrinol. Metab., May 1, 2005; 90(5): 2835 - 2842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bielawska-Pohl, C. Crola, A. Caignard, C. Gaudin, D. Dus, C. Kieda, and S. Chouaib Human NK Cells Lyse Organ-Specific Endothelial Cells: Analysis of Adhesion and Cytotoxic Mechanisms J. Immunol., May 1, 2005; 174(9): 5573 - 5582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ahmad and F. Alvarez Role of NK and NKT cells in the immunopathogenesis of HCV-induced hepatitis J. Leukoc. Biol., October 1, 2004; 76(4): 743 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. D. Perez, D. Mitchell, G. C. Jager, and G. P. Nolan LFA-1 signaling through p44/42 is coupled to perforin degranulation in CD56+CD8+ natural killer cells Blood, August 15, 2004; 104(4): 1083 - 1093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Suzui, T. Kawai, H. Kimura, K. Takeda, H. Yagita, K. Okumura, P. N. Shek, and R. J. Shephard Natural killer cell lytic activity and CD56dim and CD56bright cell distributions during and after intensive training J Appl Physiol, June 1, 2004; 96(6): 2167 - 2173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Taverna, H. Moher, D. Crowley, L. Borsig, A. Varki, and R. O. Hynes Increased primary tumor growth in mice null for {beta}3- or {beta}3/{beta}5-integrins or selectins PNAS, January 20, 2004; 101(3): 763 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Loza and B. Perussia The IL-12 Signature: NK Cell Terminal CD56+high Stage and Effector Functions J. Immunol., January 1, 2004; 172(1): 88 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Koopman, H. D. Kopcow, B. Rybalov, J. E. Boyson, J. S. Orange, F. Schatz, R. Masch, C. J. Lockwood, A. D. Schachter, P. J. Park, et al. Human Decidual Natural Killer Cells Are a Unique NK Cell Subset with Immunomodulatory Potential J. Exp. Med., October 20, 2003; 198(8): 1201 - 1212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chantakru, W.-C. Wang, M. van den Heuvel, S. Bashar, A. Simpson, Q. Chen, B. A. Croy, and S. S. Evans Coordinate Regulation of Lymphocyte-Endothelial Interactions by Pregnancy-Associated Hormones J. Immunol., October 15, 2003; 171(8): 4011 - 4019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Fehniger, M. A. Cooper, G. J. Nuovo, M. Cella, F. Facchetti, M. Colonna, and M. A. Caligiuri CD56bright natural killer cells are present in human lymph nodes and are activated by T cell-derived IL-2: a potential new link between adaptive and innate immunity Blood, April 15, 2003; 101(8): 3052 - 3057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Valteau-Couanet, C. Leboulaire, K. Maincent, M. Tournier, O. Hartmann, J. Benard, F. Beaujean, C. Boccaccio, L. Zitvogel, and E. Angevin Dendritic cells for NK/LAK activation: rationale for multicellular immunotherapy in neuroblastoma patients Blood, September 18, 2002; 100(7): 2554 - 2561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Robertson Role of chemokines in the biology of natural killer cells J. Leukoc. Biol., February 1, 2002; 71(2): 173 - 183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chantakru, C. Miller, L. E. Roach, W. A. Kuziel, N. Maeda, W.-C. Wang, S. S. Evans, and B. A. Croy Contributions from Self-Renewal and Trafficking to the Uterine NK Cell Population of Early Pregnancy J. Immunol., January 1, 2002; 168(1): 22 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Cooper, T. A. Fehniger, S. C. Turner, K. S. Chen, B. A. Ghaheri, T. Ghayur, W. E. Carson, and M. A. Caligiuri Human natural killer cells: a unique innate immunoregulatory role for the CD56bright subset Blood, May 15, 2001; 97(10): 3146 - 3151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. J. van der Vliet, N. Nishi, T. D. de Gruijl, B. M. E. von Blomberg, A. J. M. van den Eertwegh, H. M. Pinedo, G. Giaccone, and R. J. Scheper Human natural killer T cells acquire a memory-activated phenotype before birth Blood, April 1, 2000; 95(7): 2440 - 2442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Croy, A. A. Ashkar, K. Minhas, and J. D. Greenwood Can Murine Uterine Natural Killer Cells Give Insights Into the Pathogenesis of Preeclampsia? Reproductive Sciences, January 1, 2000; 7(1): 12 - 20. [Abstract] [PDF]
|
|
|
|
 |
|
 G. Nicoll, J. Ni, D. Liu, P. Klenerman, J. Munday, S. Dubock, M.-G. Mattei, and P. R. Crocker Identification and Characterization of a Novel Siglec, Siglec-7, Expressed by Human Natural Killer Cells and Monocytes J. Biol. Chem., November 26, 1999; 274(48): 34089 - 34095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Fehniger, M. H. Shah, M. J. Turner, J. B. VanDeusen, S. P. Whitman, M. A. Cooper, K. Suzuki, M. Wechser, F. Goodsaid, and M. A. Caligiuri Differential Cytokine and Chemokine Gene Expression by Human NK Cells Following Activation with IL-18 or IL-15 in Combination with IL-12: Implications for the Innate Immune Response J. Immunol., April 15, 1999; 162(8): 4511 - 4520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Evans, D. M. Schleider, L. A. Bowman, M. L. Francis, G. S. Kansas, and J. D. Black Dynamic Association of L-Selectin with the Lymphocyte Cytoskeletal Matrix J. Immunol., March 15, 1999; 162(6): 3615 - 3624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Andre, O. Spertini, S. Guia, P. Rihet, F. Dignat-George, H. Brailly, J. Sampol, P. J. Anderson, and E. Vivier Modification of P-selectin glycoprotein ligand-1 with a natural killer cell-restricted sulfated lactosamine creates an alternate ligand for L-selectin PNAS, March 28, 2000; 97(7): 3400 - 3405. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
