The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kong, Q.
Right arrow Articles by Maizels, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kong, Q.
Right arrow Articles by Maizels, N.
The Journal of Immunology, 1998, 161: 294-301.
Copyright © 1998 by The American Association of Immunologists

A {lambda} 3' Enhancer Drives Active and Untemplated Somatic Hypermutation of a {lambda}1 Transgene1

Qingzhong Kong*, Lisa Zhao2,*, Sathish Subbaiah3,* and Nancy Maizels4,*,{dagger}

Departments of * Molecular Biophysics and Biochemistry and {dagger} Genetics, Yale University, New Haven, CT 06520

Somatic hypermutation is a highly regulated process that targets mutations to the rearranged Ig genes. Little is known about the cis-elements required for somatic hypermutation of the {lambda} light chain gene. We have studied somatic hypermutation of a rearranged {lambda}1 transgene under the control of either a {lambda}2-4 or {kappa} 3' enhancer. The mutations in the transgenes were analyzed by sequencing DNA amplified from hypermutating Peyer’s patch B cells. The results indicate that the {lambda} 3' enhancer can drive active hypermutation of a {lambda}1 transgene in Peyer’s patch cells. The {lambda}1 transgene under analysis carried two marked V{lambda}2 genes immediately upstream that could serve as sequence donors in possible gene conversion events. There was no evidence of sequence transfer to the hypermutated {lambda}1 gene, suggesting that gene conversion is not a major mechanism for somatic hypermutation in mice.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
K. Malecek, J. Brandman, J. E. Brodsky, Y. Ohta, M. F. Flajnik, and E. Hsu
Somatic Hypermutation and Junctional Diversification at Ig Heavy Chain Loci in the Nurse Shark
J. Immunol., December 15, 2005; 175(12): 8105 - 8115.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. D'Avirro, D. Truong, B. Xu, and E. Selsing
Sequence Transfers between Variable Regions in a Mouse Antibody Transgene Can Occur by Gene Conversion
J. Immunol., December 15, 2005; 175(12): 8133 - 8137.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
H.-F. Tsai, N. D'Avirro, and E. Selsing
Gene conversion-like sequence transfers in a mouse antibody transgene: antigen selection allows sensitive detection of V region interactions based on homology
Int. Immunol., January 1, 2002; 14(1): 55 - 64.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
Q. Kong and N. Maizels
DNA Breaks in Hypermutating Immunoglobulin Genes: Evidence for a Break-and-Repair Pathway of Somatic Hypermutation
Genetics, May 1, 2001; 158(1): 369 - 378.
[Abstract] [Full Text]

Home page
 Nucleic Acids ResHome page
Q. Kong and N. Maizels
Breaksite batch mapping, a rapid method for assay and identification of DNA breaksites in mammalian cells
Nucleic Acids Res., March 15, 2001; 29(6): e33 - e33.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1998 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1998 by The American Association of Immunologists, Inc. All rights reserved.