| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Pathology and Program in Immunology, Tufts University School of Medicine and Sackler School of Graduate Biomedical Sciences, Boston, MA 02111
CD5 is a 67-kDa membrane glycoprotein the expression of which in murine splenic B cells is induced by surface IgM cross-linking. To analyze this induction, we transiently transfected primary splenic B cells with luciferase reporter constructs driven by various wild-type and mutated CD5 5'-flanking sequences. The transfected cells were subsequently cultured in medium with or without F(ab')2 anti-IgM (anti-IgM), and luciferase expression was assayed. Using this approach, we identified a 122-bp enhancer element necessary for anti-IgM-mediated induction of the CD5 promoter. Electrophoretic mobility shift assays indicated that four inducible and four constitutive complexes form on the enhancer fragment in nuclear extracts of primary B cells. Supershift assays revealed that two of the inducible complexes contained NFATc. Point mutations that abolished NFAT binding severely impaired enhancer function. Thus, CD5 is a target of NFAT in B cells. A third inducible complex required an intact H4TF-1 site. One of several constitutive complexes required an intact Ebox site while a second required an intact putative ets binding site. Mutation of the H4TF-1, Ebox, and Ets sites, in the presence of wild-type NFAT sites, significantly reduced the activity of the enhancer. Therefore, the induction of B cell CD5 expression requires NFAT binding and binding to at least one of three additional sites in the CD5 enhancer.
This article has been cited by other articles:
|
|
 |
|
  I. Debnath, K. M. Roundy, J. J. Weis, and J. H. Weis Defining In Vivo Transcription Factor Complexes of the Murine CD21 and CD23 Genes J. Immunol., June 1, 2007; 178(11): 7139 - 7150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. J. de Gorter, J. C. M. Vos, S. T. Pals, and M. Spaargaren The B Cell Antigen Receptor Controls AP-1 and NFAT Activity through Ras-Mediated Activation of Ral J. Immunol., February 1, 2007; 178(3): 1405 - 1414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. de la Fuente, L. Kumar, B. Lu, and R. S. Geha 3BP2 Deficiency Impairs the Response of B Cells, but Not T Cells, to Antigen Receptor Ligation. Mol. Cell. Biol., July 1, 2006; 26(14): 5214 - 5225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Renaudineau, S. Hillion, A. Saraux, R. A. Mageed, and P. Youinou An alternative exon 1 of the CD5 gene regulates CD5 expression in human B lymphocytes Blood, October 15, 2005; 106(8): 2781 - 2789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. N. Samanta, A. Palmetshofer, D. Marinkovic, T. Wirth, E. Serfling, and L. Nitschke B Cell Hyperresponsiveness and Expansion of Mature Follicular B Cells but Not of Marginal Zone B Cells in NFATc2/c3 Double-Deficient Mice J. Immunol., April 15, 2005; 174(8): 4797 - 4802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Arman, J. Calvo, M. E. Trojanowska, P. N. Cockerill, M. Santana, M. Lopez-Cabrera, J. Vives, and F. Lozano Transcriptional Regulation of Human CD5: Important Role of Ets Transcription Factors in CD5 Expression in T Cells J. Immunol., June 15, 2004; 172(12): 7519 - 7529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yang, C. H. Contag, D. Felsher, C. M. Shachaf, Y. Cao, L. A. Herzenberg, L. A. Herzenberg, and J. W. Tung The E47 transcription factor negatively regulates CD5 expression during thymocyte development PNAS, March 16, 2004; 101(11): 3898 - 3902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Berland and H. H. Wortis Normal B-1a cell development requires B cell-intrinsic NFATc1 activity PNAS, November 11, 2003; 100(23): 13459 - 13464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-C. Wong, W.-K. Chew, J. E.-L. Tan, A. J. Melendez, F. Francis, and K.-P. Lam Peritoneal CD5+ B-1 Cells Have Signaling Properties Similar to Tolerant B Cells J. Biol. Chem., August 16, 2002; 277(34): 30707 - 30715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Chumley, J. M. Dal Porto, and J. C. Cambier The Unique Antigen Receptor Signaling Phenotype of B-1 Cells Is Influenced by Locale but Induced by Antigen J. Immunol., August 15, 2002; 169(4): 1735 - 1743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Lu, R. C. Axtell, J. F. Collawn, A. Gibson, L. B. Justement, and C. Raman AP2 Adaptor Complex-Dependent Internalization of CD5: Differential Regulation in T and B Cells J. Immunol., June 1, 2002; 168(11): 5612 - 5620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ren, A. Schmalstieg, N. S. C. van Oers, and R. B. Gaynor I-{kappa}B Kinases {alpha} and {beta} Have Distinct Roles in Regulating Murine T Cell Function J. Immunol., April 15, 2002; 168(8): 3721 - 3731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Martinez-Barnetche, V. Madrid-Marina, R. A. Flavell, and J. Moreno Does CD40 Ligation Induce B Cell Negative Selection? J. Immunol., February 1, 2002; 168(3): 1042 - 1049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ren, A. Schmalstieg, D. Yuan, and R. B. Gaynor I-{kappa}B Kinase {beta} Is Critical for B Cell Proliferation and Antibody Response J. Immunol., January 15, 2002; 168(2): 577 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Liu, K.-i. Arai, and N. Arai Inhibition of NFATx Activation by an Oligopeptide: Disrupting the Interaction of NFATx with Calcineurin J. Immunol., September 1, 2001; 167(5): 2677 - 2687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. D. Bajpai, K. Zhang, M. Teutsch, R. Sen, and H. H. Wortis Bruton's Tyrosine Kinase Links the B Cell Receptor to Nuclear Factor {kappa}b Activation J. Exp. Med., May 15, 2000; 191(10): 1735 - 1744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Watanabe, S. Nisitani, K. Ikuta, M. Suzuki, T. Chiba, and T. Honjo Expression Levels of B Cell Surface Immunoglobulin Regulate Efficiency of Allelic Exclusion and Size of Autoreactive B-1 Cell Compartment J. Exp. Med., August 16, 1999; 190(4): 461 - 470. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
