| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Convertase Enzyme from Human Arthritis-Affected Cartilage: Isolation of cDNA by Differential Display, Expression of the Active Enzyme, and Regulation of TNF-1
*
Department of Rheumatology and Medicine, and
Department of Orthopedic Surgery, Hospital for Joint Diseases, New York, NY;
Skirball Institute of Biomolecular Medicine, Departments of
§
Medicine and
¶
Pathology, and
||
Kaplan Cancer Research Center, New York University Medical Center, New York, NY 10016.
A snake venom-like protease isolated by a differential display
screen between normal and osteoarthritis (OA)-affected cartilage
(designated as cSVP) has a cDNA sequence identical to TNF-
convertase enzyme (TACE). TACE shows the presence of an unknown
prodomain, a cysteine switch, a catalytic domain, a zinc binding
region, a disintegrin region, an EGF-like domain, a transmembrane
domain, and a unique cytoplasmic region. A TACE construct harboring the
signal + prodomain + catalytic region (TACE-SPC
DETCy),
expressed in baculovirus could cleave preferentially (
12-fold) the
TNF-specific peptide over the matrix metalloproteases peptide in vitro.
This recombinant protein also cleaved the natural substrate
GST-ProTNF- to TNF- (17 kDa) in vitro. The mRNA for TACE, which
is broadly distributed and differentially expressed in a variety of
human tissues, is up-regulated in arthritis-affected cartilage, but not
normal cartilage. OA-affected cartilage also expressed TNF- mRNA
that was not detected in normal cartilage. The OA-affected cartilage
(in explant assays) spontaneously released TNF- and IL-8 in ex vivo
conditions. Addition of TNF-R fused to IgG Fc fragment (TNF-R:Fc)
in the presence or absence of soluble IL-1R (with which it acted
additively) significantly attenuated the spontaneous/autocrine release
of articular IL-8 in this assay. These experiments demonstrate a
functional paracrine/autocrine role of TNF- in OA-affected cartilage
that may depend, in part, on up-regulated levels of chondrocyte-derived
TACE.
This article has been cited by other articles:
|
|
 |
|
  Y. Wang, A. H. Herrera, Y. Li, K. K. Belani, and B. Walcheck Regulation of Mature ADAM17 by Redox Agents for L-Selectin Shedding J. Immunol., February 15, 2009; 182(4): 2449 - 2457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. M. Reddy, K. V. Ramana, S. Srivastava, A. Bhatnagar, and S. K. Srivastava Aldose Reductase Regulates High Glucose-Induced Ectodomain Shedding of Tumor Necrosis Factor (TNF)-{alpha} via Protein Kinase C-{delta} and TNF-{alpha} Converting Enzyme in Vascular Smooth Muscle Cells Endocrinology, January 1, 2009; 150(1): 63 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Charbonneau, K. Harper, F. Grondin, M. Pelmus, P. P. McDonald, and C. M. Dubois Hypoxia-inducible Factor Mediates Hypoxic and Tumor Necrosis Factor {alpha}-induced Increases in Tumor Necrosis Factor-{alpha} Converting Enzyme/ADAM17 Expression by Synovial Cells J. Biol. Chem., November 16, 2007; 282(46): 33714 - 33724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Bohgaki, Y Amasaki, N Nishimura, M Bohgaki, Y Yamashita, M Nishio, K-i Sawada, S Jodo, T Atsumi, and T Koike Up regulated expression of tumour necrosis factor {alpha} converting enzyme in peripheral monocytes of patients with early systemic sclerosis Ann Rheum Dis, August 1, 2005; 64(8): 1165 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li and H. Fan Loss of Ectodomain Shedding Due to Mutations in the Metalloprotease and Cysteine-rich/Disintegrin Domains of the Tumor Necrosis Factor-{alpha} Converting Enzyme (TACE) J. Biol. Chem., June 25, 2004; 279(26): 27365 - 27375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. V. Bax, A. J. Messent, J. Tart, M. van Hoang, J. Kott, R. A. Maciewicz, and M. J. Humphries Integrin {alpha}5{beta}1 and ADAM-17 Interact in Vitro and Co-localize in Migrating HeLa Cells J. Biol. Chem., May 21, 2004; 279(21): 22377 - 22386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. F. Seals and S. A. Courtneidge The ADAMs family of metalloproteases: multidomain proteins with multiple functions Genes & Dev., January 1, 2003; 17(1): 7 - 30. [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen, K. Diener, I. R. Patel, J. K. Kawooya, G. A. Martin, P. Yamdagni, X. Zhang, A. Sandrasagra, S. Sahasrabudhe, and S. J. Busch Phage Display of Functional Human TNF-{alpha} Converting Enzyme Catalytic Domain: A Rapid Method for the Production of Stabilized Proteolytic Proteins for Assay Development and High-Throughput Screening J Biomol Screen, October 1, 2002; 7(5): 433 - 440. [Abstract] [PDF]
|
|
|
|
 |
|
 J Brynskov, P Foegh, G Pedersen, C Ellervik, T Kirkegaard, A Bingham, and T Saermark Tumour necrosis factor {alpha} converting enzyme (TACE) activity in the colonic mucosa of patients with inflammatory bowel disease Gut, July 1, 2002; 51(1): 37 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Attur, M. N. Dave, R. M. Clancy, I. R. Patel, S. B. Abramson, and A. R. Amin Functional Genomic Analysis in Arthritis-Affected Cartilage: Yin-Yang Regulation of Inflammatory Mediators by {alpha}5{beta}1 and {alpha}V{beta}3 Integrins J. Immunol., March 1, 2000; 164(5): 2684 - 2691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, A. R. Johnson, Q.-Z. Ye, and R. D. Dyer Catalytic Activities and Substrate Specificity of the Human Membrane Type 4 Matrix Metalloproteinase Catalytic Domain J. Biol. Chem., November 12, 1999; 274(46): 33043 - 33049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Solomon, N. Pesti, G. Wu, and R. C. Newton Cutting Edge: A Dominant Negative Form of TNF-{alpha} Converting Enzyme Inhibits ProTNF and TNFRII Secretion J. Immunol., October 15, 1999; 163(8): 4105 - 4108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Roberts, P. H. Tani, L. C. Bridges, Z. Laszik, and R. D. Bowditch MDC-L, a Novel Metalloprotease Disintegrin Cysteine-rich Protein Family Member Expressed by Human Lymphocytes J. Biol. Chem., October 8, 1999; 274(41): 29251 - 29259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Garton, P. J. Gough, C. P. Blobel, G. Murphy, D. R. Greaves, P. J. Dempsey, and E. W. Raines Tumor Necrosis Factor-alpha -converting Enzyme (ADAM17) Mediates the Cleavage and Shedding of Fractalkine (CX3CL1) J. Biol. Chem., October 5, 2001; 276(41): 37993 - 38001. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
