The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smith, D.
Right arrow Articles by Kaplan, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smith, D.
Right arrow Articles by Kaplan, D.
Right arrowPubmed/NCBI databases
*Substance via MeSH
The Journal of Immunology, 1998, 160: 4159-4160.
Copyright © 1998 by The American Association of Immunologists

Technical Note: Aberrant Detection of Cell Surface Fas Ligand with Anti-Peptide Antibodies

Dawn Smith, Scott Sieg and David Kaplan1

Institute of Pathology, Case Western Reserve University, Cleveland, OH 44120.

Polyclonal rabbit Abs raised against peptides from the C-terminal region (the extracellular domain) of human Fas ligand were produced for the detection of the molecule in Western blot analysis and immunohistochemistry. These Abs have been used by several groups of investigators to assess cell surface Fas ligand via flow cytometry, but we show that these polyclonal rabbit Abs do not detect cell surface Fas ligand by that technique.




This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
F. Donskov, H. von der Maase, N. Marcussen, S. Hamilton-Dutoit, H. H. T. Madsen, J. J. Jensen, and M. Hokland
Fas Ligand Expression in Metastatic Renal Cell Carcinoma During Interleukin-2 Based Immunotherapy: No In vivo Effect of Fas Ligand Tumor Counterattack
Clin. Cancer Res., December 1, 2004; 10(23): 7911 - 7916.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
D. Chopin, R. Barei-Moniri, P. Maille, M.-A. Le Frere-Belda, B. Muscatelli-Groux, N. Merendino, L. Lecerf, A. Stoppacciaro, and F. Velotti
Human Urinary Bladder Transitional Cell Carcinomas Acquire the Functional Fas Ligand during Tumor Progression
Am. J. Pathol., April 1, 2003; 162(4): 1139 - 1149.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
N. Kanuga, H. L. Winton, L. Beauchene, A. Koman, A. Zerbib, S. Halford, P.-O. Couraud, D. Keegan, P. Coffey, R. D. Lund, et al.
Characterization of Genetically Modified Human Retinal Pigment Epithelial Cells Developed for In Vitro and Transplantation Studies
Invest. Ophthalmol. Vis. Sci., February 1, 2002; 43(2): 546 - 555.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
K. Maedler, G. A. Spinas, R. Lehmann, P. Sergeev, M. Weber, A. Fontana, N. Kaiser, and M. Y. Donath
Glucose Induces {beta}-Cell Apoptosis Via Upregulation of the Fas Receptor in Human Islets
Diabetes, August 1, 2001; 50(8): 1683 - 1690.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. Baran, K. Weglarczyk, M. Mysiak, K. Guzik, M. Ernst, H.-D. Flad, and J. Pryjma
Fas (CD95)-Fas Ligand Interactions Are Responsible for Monocyte Apoptosis Occurring as a Result of Phagocytosis and Killing of Staphylococcus aureus
Infect. Immun., March 1, 2001; 69(3): 1287 - 1297.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Fishman, B. Irena, S. Kellman-Pressman, M. Karas, and S. Segal
The role of MHC class I glycoproteins in the regulation of induction of cell death in immunocytes by malignant melanoma cells
PNAS, February 1, 2001; (2001) 41591298.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
S. Jodo, A. M. Hohlbaum, S. Xiao, D. Chan, D. Strehlow, D. H. Sherr, A. Marshak-Rothstein, and S.-T. Ju
CD95 (Fas) Ligand-Expressing Vesicles Display Antibody-Mediated, FcR-Dependent Enhancement of Cytotoxicity
J. Immunol., November 15, 2000; 165(10): 5487 - 5494.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Favre-Felix, A. Fromentin, A. Hammann, E. Solary, F. Martin, and B. Bonnotte
Cutting Edge: The Tumor Counterattack Hypothesis Revisited: Colon Cancer Cells Do Not Induce T Cell Apoptosis Via the Fas (CD95, APO-1) Pathway
J. Immunol., May 15, 2000; 164(10): 5023 - 5027.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
L. Mincheva-Nilsson, O. Nagaeva, K.-G. Sundqvist, M.-L. Hammarstrom, S. Hammarstrom, and V. Baranov
{gamma}{delta}T cells of human early pregnancy decidua: evidence for cytotoxic potency
Int. Immunol., May 1, 2000; 12(5): 585 - 596.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Sata, T. Suhara, and K. Walsh
Vascular Endothelial Cells and Smooth Muscle Cells Differ in Expression of Fas and Fas Ligand and in Sensitivity to Fas Ligand-Induced Cell Death : Implications for Vascular Disease and Therapy
Arterioscler Thromb Vasc Biol, February 1, 2000; 20(2): 309 - 316.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
P. L. Arscott, T. Stokes, A. Myc, T. J. Giordano, N. W. Thompson, and J. R. Baker Jr.
Fas (CD95) Expression is Up-Regulated on Papillary Thyroid Carcinoma
J. Clin. Endocrinol. Metab., November 1, 1999; 84(11): 4246 - 4252.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
B. R. Gastman, Y. Atarashi, T. E. Reichert, T. Saito, L. Balkir, H. Rabinowich, and T. L. Whiteside
Fas Ligand Is Expressed on Human Squamous Cell Carcinomas of the Head and Neck, and It Promotes Apoptosis of T Lymphocytes
Cancer Res., October 1, 1999; 59(20): 5356 - 5364.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. D. Bretz, P. L. Arscott, A. Myc, and J. R. Baker Jr.
Inflammatory Cytokine Regulation of Fas-mediated Apoptosis in Thyroid Follicular Cells
J. Biol. Chem., September 3, 1999; 274(36): 25433 - 25438.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Kayagaki, N. Yamaguchi, M. Nakayama, K. Takeda, H. Akiba, H. Tsutsui, H. Okamura, K. Nakanishi, K. Okumura, and H. Yagita
Expression and Function of TNF-Related Apoptosis-Inducing Ligand on Murine Activated NK Cells
J. Immunol., August 15, 1999; 163(4): 1906 - 1913.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
R. G. Uzzo, P. Rayman, V. Kolenko, P. E. Clark, T. Bloom, A. M. Ward, L. Molto, C. Tannenbaum, L. J. Worford, R. Bukowski, et al.
Mechanisms of Apoptosis in T Cells from Patients with Renal Cell Carcinoma
Clin. Cancer Res., May 1, 1999; 5(5): 1219 - 1229.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Micheau, E. Solary, A. Hammann, and M.-T. Dimanche-Boitrel
Fas Ligand-independent, FADD-mediated Activation of the Fas Death Pathway by Anticancer Drugs
J. Biol. Chem., March 19, 1999; 274(12): 7987 - 7992.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Z. Zaks, D. B. Chappell, S. A. Rosenberg, and N. P. Restifo
Fas-Mediated Suicide of Tumor-Reactive T Cells Following Activation by Specific Tumor: Selective Rescue by Caspase Inhibition
J. Immunol., March 15, 1999; 162(6): 3273 - 3279.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. B. Chappell, T. Z. Zaks, S. A. Rosenberg, and N. P. Restifo
Human Melanoma Cells Do Not Express Fas (Apo-1/CD95) Ligand
Cancer Res., January 1, 1999; 59(1): 59 - 62.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. B. Brown and J. Savill
Phagocytosis Triggers Macrophage Release of Fas Ligand and Induces Apoptosis of Bystander Leukocytes
J. Immunol., January 1, 1999; 162(1): 480 - 485.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Fishman, B. Irena, S. Kellman-Pressman, M. Karas, and S. Segal
The role of MHC class I glycoproteins in the regulation of induction of cell death in immunocytes by malignant melanoma cells
PNAS, February 13, 2001; 98(4): 1740 - 1744.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1998 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1998 by The American Association of Immunologists, Inc. All rights reserved.