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Allelic Exclusion During T Cell Differentiation1
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037
We have previously shown that phenotypic allelic exclusion of TCR
-chain is functional only in mature thymocytes. A significant
proportion of immature thymocytes (TCRlow) express
more than one cell surface -chain, but mature thymocytes
(TCRhigh) show phenotypic allelic exclusion and express
only a single -chain. We have analyzed thymocytes for both surface
and intracellular -chain expression and find that the majority of
mature thymocytes express a second -chain intracellularly. This
result is predicted by a model in which the developmentally regulated
allelic exclusion of the TCR -chain is caused by competition between
-chains for the ß-chain rather than by models in which one
-chain is down-regulated or in which selection favors cells with
only a single -chain species. Changes in the relative amounts of
- and ß-chains available for pairing may therefore allow
competition between the two -chains for the ß-chain. Peripheral T
cells also frequently express second -chains in the cytoplasm
(18–27%), despite a rather low frequency of dual -chain expression
on the cell surface (2–4%). The frequency of nonsurface expressed
-chains is reduced somewhat compared with thymocytes, indicating
that an additional level of control of allelic exclusion operates
during the maturation of peripheral T cells.
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