| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Biomedical Sciences and Human Oncology, Section of Internal Medicine and Clinical Oncology, University of Bari Medical School, Bari, and
Department of Experimental Oncology I, Centro di Riferimento Oncologico, Aviano, Italy
Clonal rearrangements of Ig heavy chain (IgH) genes and hepatitis C virus (HCV) genomic sequences were assayed on intrahepatic B lymphocytes isolated from HCV chronically infected patients with and without type II mixed cryoglobulinemia (MC). Liver tissue samples from eight patients with and nine without MC were subjected to routine histologic studies, immunophenotyping, and genotypic analysis including IgH V-D-J region gene rearrangements by PCR. RT-PCR, signal amplification by branched DNA assay, and in situ hybridization technique were used to detect and quantitate HCV RNA genomic sequences in selected B cells purified from each tissue sample. Although HCV infection of intrahepatic B cells was shown in all patients both with and without MC, frank B cell monoclonal and oligoclonal patterns were found in only three and four patients with MC, respectively. No monoclonal profile was seen in the noncryoglobulinemic patients, whereas an oligoclonal profile was demonstrated in four of them. No clonalities were shown in HCV-unrelated patients matched for age and severity of liver disease. No obvious difference in HCV genotype distribution was found in relation to the clonal expansion profile. Noncryoglobulinemic patients showing clonal expansion in liver tissue had higher titers of serum rheumatoid factor (RF). Spontaneous production of RF was shown in cell cultures of intrahepatic B cells, suggesting their persistent stimulation in vivo. These data indicate that HCV infection of B cells and B cell clonal expansions occur in the liver microenvironment and preferentially involve RF-producing cells.
This article has been cited by other articles:
|
|
 |
|
  D. Sansonno, F. A. Tucci, L. Troiani, G. Lauletta, M. Montrone, V. Conteduca, L. Sansonno, and F. Dammacco Increased serum levels of the chemokine CXCL13 and up-regulation of its gene expression are distinctive features of HCV-related cryoglobulinemia and correlate with active cutaneous vasculitis Blood, September 1, 2008; 112(5): 1620 - 1627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Charles, R. M. Green, S. Marukian, A. H. Talal, G. V. Lake-Bakaar, I. M. Jacobson, C. M. Rice, and L. B. Dustin Clonal expansion of immunoglobulin M+CD27+ B cells in HCV-associated mixed cryoglobulinemia Blood, February 1, 2008; 111(3): 1344 - 1356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D S Viswanatha and A Dogan Hepatitis C virus and lymphoma J. Clin. Pathol., December 1, 2007; 60(12): 1378 - 1383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Sansonno, A. Carbone, V. De Re, and F. Dammacco Hepatitis C virus infection, cryoglobulinaemia, and beyond Rheumatology, April 1, 2007; 46(4): 572 - 578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sene, N. Limal, P. Ghillani-Dalbin, D. Saadoun, J.-C. Piette, and P. Cacoub Hepatitis C virus-associated B-cell proliferation--the role of serum B lymphocyte stimulator (BLyS/BAFF) Rheumatology, January 1, 2007; 46(1): 65 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Wolk, C. Gremion, N. Ivashkina, O. B. Engler, B. Grabscheid, E. Bieck, H. E. Blum, A. Cerny, and D. Moradpour Stable human lymphoblastoid cell lines constitutively expressing hepatitis C virus proteins J. Gen. Virol., June 1, 2005; 86(6): 1737 - 1746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. H. Sasso, M. Martinez, S. L. Yarfitz, P. Ghillani, L. Musset, J.-C. Piette, and P. Cacoub Frequent Joining of Bcl-2 to a JH6 Gene in Hepatitis C Virus-Associated t(14;18) J. Immunol., September 1, 2004; 173(5): 3549 - 3556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Zuckerman, A. Kessel, G. Slobodin, E. Sabo, D. Yeshurun, and E. Toubi Antiviral Treatment Down-Regulates Peripheral B-Cell CD81 Expression and CD5 Expansion in Chronic Hepatitis C Virus Infection J. Virol., October 1, 2003; 77(19): 10432 - 10436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sansonno, V. De Re, G. Lauletta, F. A. Tucci, M. Boiocchi, and F. Dammacco Monoclonal antibody treatment of mixed cryoglobulinemia resistant to interferon alpha with an anti-CD20 Blood, May 15, 2003; 101(10): 3818 - 3826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Casato, C. Mecucci, V. Agnello, M. Fiorilli, G. B. Knight, C. Matteucci, L. Gao, and J. Kay Regression of lymphoproliferative disorder after treatment for hepatitis C virus infection in a patient with partial trisomy 3, Bcl-2 overexpression, and type II cryoglobulinemia Blood, March 15, 2002; 99(6): 2259 - 2261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Racanelli, D. Sansonno, C. Piccoli, F. P. D'Amore, F. A. Tucci, and F. Dammacco Molecular Characterization of B Cell Clonal Expansions in the Liver of Chronically Hepatitis C Virus-Infected Patients J. Immunol., July 1, 2001; 167(1): 21 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. De Re, S. De Vita, A. Marzotto, M. Rupolo, A. Gloghini, B. Pivetta, D. Gasparotto, A. Carbone, and M. Boiocchi Sequence analysis of the immunoglobulin antigen receptor of hepatitis C virus-associated non-Hodgkin lymphomas suggests that the malignant cells are derived from the rheumatoid factor-producing cells that occur mainly in type II cryoglobulinemia Blood, November 15, 2000; 96(10): 3578 - 3584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sansonno, C. Lotesoriere, V. Cornacchiulo, M. Fanelli, P. Gatti, G. Iodice, V. Racanelli, and F. Dammacco Hepatitis C Virus Infection Involves CD34+ Hematopoietic Progenitor Cells in Hepatitis C Virus Chronic Carriers Blood, November 1, 1998; 92(9): 3328 - 3337. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
