HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Waldman, W. J. Articles by Huang, E. H. Search for Related Content PubMed PubMed Citation Articles by Waldman, W. J. Articles by Huang, E. H.

An In Vitro Model of T Cell Activation by Autologous Cytomegalovirus (CMV)-Infected Human Adult Endothelial Cells: Contribution of CMV-Enhanced Endothelial ICAM-1¹

W. James Waldman^2,*, Deborah A. Knight^* and Emina H. Huang

Departments of ^* Pathology and Surgery, The Ohio State University College of Medicine, Columbus, OH 43210

Cellular immunity is strongly implicated in control of CMV disease; however, many mechanistic details remain unresolved. We previously demonstrated T cell activation responses to CMV-infected allogeneic endothelial cells (EC), suggesting EC as a mediator of CMV response in the transplant recipient. We now test the hypothesis that CMV-specific T cell responses can be directly stimulated by infected EC in an environment free of potentially confounding allogeneic factors. By isolating splenic T cells and gonadal vein endothelial cells (GVEC) from individual cadaveric organ donors, we have developed an in vitro model of T cell interaction with autologous CMV-infected EC. Proliferation assays demonstrated significantly enhanced responses by CMV-seropositive donor-derived T cells cocultured with CMV-infected GVEC, as compared with those elicited by uninfected cells. Similarly, as determined by limiting dilution analysis of IL-2-producing cells, T cell response frequencies to infected GVEC were significantly greater than to uninfected EC. In contrast, responses of CMV-seronegative donor-derived T cells were minimal, regardless of CMV status of stimulator GVEC. Intriguingly, CD4 responses were observed in spite of the fact that CMV-infected EC express no HLA class II. Finally, attenuation of CMV-stimulated T cell proliferation observed in the presence of blocking Ab specific for ICAM-1 suggests a contributing role for CMV-enhanced endothelial ICAM-1 expression in the activation response. These studies demonstrate that EC can stimulate autologous T cell responses to CMV in the absence of accessory APC and suggest potentially novel mechanisms of immune activation.

This article has been cited by other articles:

				M. E.J. Reinders, T. J. Rabelink, and D. M. Briscoe Angiogenesis and Endothelial Cell Repair in Renal Disease and Allograft Rejection J. Am. Soc. Nephrol., April 1, 2006; 17(4): 932 - 942. [Abstract] [Full Text] [PDF]

				M. Leis, M. Marschall, and T. Stamminger Downregulation of the cellular adhesion molecule Thy-1 (CD90) by cytomegalovirus infection of human fibroblasts J. Gen. Virol., July 1, 2004; 85(7): 1995 - 2000. [Abstract] [Full Text] [PDF]

				M. Weis, T. N. Kledal, K. Y. Lin, S. N. Panchal, S. Z. Gao, H. A. Valantine, E. S. Mocarski, and J. P. Cooke Cytomegalovirus Infection Impairs the Nitric Oxide Synthase Pathway: Role of Asymmetric Dimethylarginine in Transplant Arteriosclerosis Circulation, February 3, 2004; 109(4): 500 - 505. [Abstract] [Full Text] [PDF]

				M. Kronschnabl and T. Stamminger Synergistic induction of intercellular adhesion molecule-1 by the human cytomegalovirus transactivators IE2p86 and pp71 is mediated via an Sp1-binding site J. Gen. Virol., January 1, 2003; 84(1): 61 - 73. [Abstract] [Full Text] [PDF]

				J. B. Sundstrom, L. K. McMullan, C. F. Spiropoulou, W. C. Hooper, A. A. Ansari, C. J. Peters, and P. E. Rollin Hantavirus Infection Induces the Expression of RANTES and IP-10 without Causing Increased Permeability in Human Lung Microvascular Endothelial Cells J. Virol., July 1, 2001; 75(13): 6070 - 6085. [Abstract] [Full Text] [PDF]

				M. K. Froberg, N. Seacotte, and E. Dahlberg Cytomegalovirus Seropositivity and Serum Total Cholesterol Levels in Young Patients Ann. Clin. Lab. Sci., April 1, 2001; 31(2): 157 - 161. [Abstract] [Full Text] [PDF]

				C. A. Labarrere, D. R. Nelson, S. J. Miller, J. M. Nieto, J. A. Conner, D. E. Pitts, P. C. Kirlin, and H. G. Halbrook Value of Serum-Soluble Intercellular Adhesion Molecule-1 for the Noninvasive Risk Assessment of Transplant Coronary Artery Disease, Posttransplant Ischemic Events, and Cardiac Graft Failure Circulation, September 26, 2000; 102(13): 1549 - 1555. [Abstract] [Full Text] [PDF]

				M. K. Froberg, A. Adams, N. Seacotte, J. Parker-Thornburg, and P. Kolattukudy Cytomegalovirus Infection Accelerates Inflammation in Vascular Tissue Overexpressing Monocyte Chemoattractant Protein-1 Circ. Res., December 7, 2001; 89(12): 1224 - 1230. [Abstract] [Full Text] [PDF]