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Loyola University Chicago, Stritch School of Medicine, Department of Microbiology and Immunology, Maywood, IL 60153
By 
4 to 8 wk of age, the IgH VDJ genes of essentially all rabbit
B lymphocytes have undergone somatic diversification. Some of this
diversification occurs in the appendix, which is a gut-associated
lymphoid tissue (GALT). To determine whether GALT is essential for
somatic diversification, we surgically removed the appendix, sacculus
rotundus, and Peyer’s patches from neonatal rabbits (designated
GALT-less) and examined the extent to which VDJ genes were somatically
diversified. We found that the IgM VDJ genes of peripheral B cells from
2- to 5-mo-old GALT-less rabbits had undergone considerably less
somatic diversification than those of control rabbits. Further, the
percentage of peripheral B cells in the GALT-less rabbits was generally
less than that of controls. Our data suggest that, in rabbits, the
primary Ab repertoire develops in GALT, and B cell expansion also
occurs there. Hence, GALT may function as a mammalian bursal homologue.
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