| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Pathology, School of Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030
Mouse bone marrow (BM) stromal cell conditioned medium (CM) from our long-term lymphoid culture system selectively induces the in vitro proliferation and presumptive differentiation of pre-pro-B cells (B220+, HSA-, TdT- or TdT+, cµ-) from adult rat, mouse, and human BM. However, the responsible growth factor(s) has not yet been identified. Inasmuch as IL-7 is one of the cytokines most closely associated with early B-lineage development, we utilized BM adherent cells and stromal cell lines from IL-7 gene-deleted (-/-) mice in combination with rIL-7 and anti-IL-7 mAb to investigate its possible regulatory role in our culture system. The results show that, although rIL-7 and IL-7 (-/-) CM each can maintain the viability of freshly harvested pre-pro-B cells in vitro, neither induces them to proliferate and/or differentiate, even in the presence of recombinant stem cell factor (rSCF) and/or recombinant insulin-like growth factor (rIGF). The results also show that anti-IL-7 mAb fails to neutralize the pre-pro-B cell growth-stimulating activity in IL-7 (+/+) CM. Yet rIL-7 enables IL-7 (-/-) CM to induce proliferation of pre-pro-B cells, and to "prime" them to respond directly to monomeric IL-7. Furthermore, anti-IL-7 mAb adsorbs the pre-pro-B cell growth-stimulating activity from both IL-7 (+/+) CM and rIL-7-supplemented IL-7 (-/-) CM; but rIL-7 does not restore this activity. Lastly, both pre-pro-B cell growth-stimulatory activity and IL-7 are quantitatively recovered by ultrafiltration in the 50 to 100 kDa, rather than the 10 to 50 kDa, apparent molecular mass fraction. These results suggest that the pre-pro-B cell growth-stimulating activity in our culture system is the property of a self-associating complex of IL-7 and a second BM stromal cell-derived cofactor.
This article has been cited by other articles:
|
|
 |
|
  C. L. Mackall A fine romance: IL-7 and HGFbeta Blood, March 1, 2006; 107(5): 1739 - 1740. [Full Text] [PDF]
|
|
|
|
|
|
L. Lai, R. A. Zeff, and I. Goldschneider A recombinant single-chain IL-7/HGFbeta hybrid cytokine induces juxtacrine interactions of the IL-7 and HGF (c-Met) receptors and stimulates the proliferation of CFU-S12, CLPs, and pre-pro-B cells Blood, March 1, 2006; 107(5): 1776 - 1784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lai and I. Goldschneider Cutting Edge: Identification of a Hybrid Cytokine Consisting of IL-7 and the {beta}-Chain of the Hepatocyte Growth Factor/Scatter Factor J. Immunol., October 1, 2001; 167(7): 3550 - 3554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Martinez-M., S. Minguet, P. Gonzalo, P. G. Soro, B. de Andres, A. Izcue, M. A. R. Marcos, and M.-L. Gaspar Long-lived polyclonal B-cell lines derived from midgestation mouse embryo lymphohematopoietic progenitors reconstitute adult immunodeficient mice Blood, September 15, 2001; 98(6): 1862 - 1871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wei, R. Zeff, and I. Goldschneider Murine Pro-B Cells Require IL-7 and Its Receptor Complex to Up-Regulate IL-7R{alpha}, Terminal Deoxynucleotidyltransferase, and c{micro} Expression J. Immunol., February 15, 2000; 164(4): 1961 - 1970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. de Andres, A. L. Mueller, S. Verbeek, M. Sandor, and R. G. Lynch A Regulatory Role for Fcgamma Receptors CD16 and CD32 in the Development of Murine B Cells Blood, October 15, 1998; 92(8): 2823 - 2829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lai, F. Chen, S. McKenna, and I. Goldschneider Identification of an IL-7-Associated Pre-Pro-B Cell Growth-Stimulating Factor (PPBSF). II. PPBSF Is a Covalently Linked Heterodimer of IL-7 and a Mr 30,000 Cofactor J. Immunol., March 1, 1998; 160(5): 2280 - 2286. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
