| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322
The natural mouse pathogen polyoma virus is highly oncogenic in H-2k mice carrying the endogenous superantigen encoded by the mouse mammary tumor provirus Mtv-7. This superantigen results in deletion of Vß6 TCR-expressing polyoma-specific CD8+ CTL, which appear to be critical effectors against polyoma tumorigenesis. Here we have isolated cloned lines of CD8+ T cells from resistant (i.e., Mtv-7-) H-2k mice that specifically lyse syngeneic polyoma virus-infected cells and polyoma tumor cells. Nearly all these CTL clones express Vß6 and are restricted in their recognition of virus-infected cells by H-2Dk. Screening a panel of synthetic peptides predicted to bind to Dk, for which no consensus peptide binding motif is known, we identified a peptide corresponding to a nine-amino acid sequence in the carboxyl-terminus of the middle T (MT) protein (amino acids 389–397) that was recognized by all the Vß6+CD8+ CTL clones. The inability of MT389–397-reactive CTL to recognize cells infected with a mutant polyoma virus encoding a MT truncated just proximal to this sequence indicates that MT389-397 is a naturally processed peptide. The frequencies of precursor CTL specific for polyoma virus and MT389–397 peptide were similar, indicating that MT389–397 is the immunodominant epitope in H-2k mice. In addition, polyoma-infected resistant mice possess a 10- to 20-fold higher MT389-397-specific precursor CTL frequency than susceptible mice. This highly focused CTL response to polyoma virus provides a valuable animal model to investigate the in vivo activity of CTL against virus-induced neoplasia.
This article has been cited by other articles:
|
|
 |
|
  N. Escande-Beillard, L. Washburn, D. Zekzer, Z.-P. Wu, S. Eitan, S. Ivkovic, Y. Lu, H. Dang, B. Middleton, T. V. Bilousova, et al. Neurons Preferentially Respond to Self-MHC Class I Allele Products Regardless of Peptide Presented J. Immunol., January 15, 2010; 184(2): 816 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Swanson II, C. D. Pack, A. Hadley, C.-R. Wang, I. Stroynowski, P. E. Jensen, and A. E. Lukacher An MHC class Ib-restricted CD8 T cell response confers antiviral immunity J. Exp. Med., July 7, 2008; 205(7): 1647 - 1657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. P. Andrews, C. D. Pack, and A. E. Lukacher Generation of Antiviral Major Histocompatibility Complex Class I-Restricted T Cells in the Absence of CD8 Coreceptors J. Virol., May 15, 2008; 82(10): 4697 - 4705. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Z. Shen, A. E. Lukacher, S. Billet, I. R. Williams, and K. E. Bernstein Expression of Angiotensin-converting Enzyme Changes Major Histocompatibility Complex Class I Peptide Presentation by Modifying C Termini of Peptide Precursors J. Biol. Chem., April 11, 2008; 283(15): 9957 - 9965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dall and S. Hariharan BK Virus Nephritis after Renal Transplantation Clin. J. Am. Soc. Nephrol., March 1, 2008; 3(Supplement_2): S68 - S75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Kemball, E. Szomolanyi-Tsuda, and A. E. Lukacher Allogeneic Differences in the Dependence on CD4+ T-Cell Help for Virus-Specific CD8+ T-Cell Differentiation J. Virol., December 15, 2007; 81(24): 13743 - 13753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Kemball, C. D. Pack, H. M. Guay, Z.-N. Li, D. A. Steinhauer, E. Szomolanyi-Tsuda, and A. E. Lukacher The Antiviral CD8+ T Cell Response Is Differentially Dependent on CD4+ T Cell Help Over the Course of Persistent Infection J. Immunol., July 15, 2007; 179(2): 1113 - 1121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. P. Andrews, C. D. Pack, V. Vezys, G. N. Barber, and A. E. Lukacher Early Virus-Associated Bystander Events Affect the Fitness of the CD8 T Cell Response to Persistent Virus Infection J. Immunol., June 1, 2007; 178(11): 7267 - 7275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Byers, N. P. Andrews, and A. E. Lukacher CD94/NKG2A Expression Is Associated with Proliferative Potential of CD8 T Cells during Persistent Polyoma Virus Infection J. Immunol., May 15, 2006; 176(10): 6121 - 6129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Kemball, E. D. H. Lee, E. Szomolanyi-Tsuda, T. C. Pearson, C. P. Larsen, and A. E. Lukacher Costimulation Requirements for Antiviral CD8+ T Cells Differ for Acute and Persistent Phases of Polyoma Virus Infection J. Immunol., February 1, 2006; 176(3): 1814 - 1824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Velupillai, R. L. Garcea, and T. L. Benjamin Polyoma Virus-Like Particles Elicit Polarized Cytokine Responses in APCs from Tumor-Susceptible and -Resistant Mice J. Immunol., January 15, 2006; 176(2): 1148 - 1153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Millrain, D. Scott, C. Addey, H. Dewchand, P. Ellis, I. Ehrmann, M. Mitchell, P. Burgoyne, E. Simpson, and J. Dyson Identification of the Immunodominant HY H2-Dk Epitope and Evaluation of the Role of Direct and Indirect Antigen Presentation in HY Responses J. Immunol., December 1, 2005; 175(11): 7209 - 7217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Kemball, E. D. H. Lee, V. Vezys, T. C. Pearson, C. P. Larsen, and A. E. Lukacher Late Priming and Variability of Epitope-Specific CD8+ T Cell Responses during a Persistent Virus Infection J. Immunol., June 15, 2005; 174(12): 7950 - 7960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Comoli, S. Basso, A. Azzi, A. Moretta, R. De Santis, F. Del Galdo, R. De Palma, U. Valente, A. Nocera, F. Perfumo, et al. Dendritic Cells Pulsed with Polyomavirus BK Antigen Induce Ex Vivo Polyoma BK Virus-Specific Cytotoxic T-Cell Lines in Seropositive Healthy Individuals and Renal Transplant Recipients J. Am. Soc. Nephrol., December 1, 2003; 14(12): 3197 - 3204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Byers, C. C. Kemball, J. M. Moser, and A. E. Lukacher Cutting Edge: Rapid In Vivo CTL Activity by Polyoma Virus-Specific Effector and Memory CD8+ T Cells J. Immunol., July 1, 2003; 171(1): 17 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kambayashi, E. Assarsson, A. E. Lukacher, H.-G. Ljunggren, and P. E. Jensen Memory CD8+ T Cells Provide an Early Source of IFN-{gamma} J. Immunol., March 1, 2003; 170(5): 2399 - 2408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Velupillai, J. P. Carroll, and T. L. Benjamin Susceptibility to Polyomavirus-Induced Tumors in Inbred Mice: Role of Innate Immune Responses J. Virol., October 1, 2002; 76(19): 9657 - 9663. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lomas, E. Hanon, Y. Tanaka, C. R. M. Bangham, and K. G. Gould Presentation of a new H-2Dk-restricted epitope in the Tax protein of human T-lymphotropic virus type I is enhanced by the proteasome inhibitor lactacystin J. Gen. Virol., March 1, 2002; 83(3): 641 - 650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Schamel, P. Staeheli, and J. Hausmann Identification of the Immunodominant H-2Kk-Restricted Cytotoxic T-Cell Epitope in the Borna Disease Virus Nucleoprotein J. Virol., September 15, 2001; 75(18): 8579 - 8588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tourdot, S. Herath, and K. G. Gould Characterization of a new H-2Dk-restricted epitope prominent in primary influenza A virus infection J. Gen. Virol., July 1, 2001; 82(7): 1749 - 1755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Gottlieb and L. P. Villarreal Natural Biology of Polyomavirus Middle T Antigen Microbiol. Mol. Biol. Rev., June 1, 2001; 65(2): 288 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Makrigiannis, A. T. Pau, A. Saleh, R. Winkler-Pickett, J. R. Ortaldo, and S. K. Anderson Class I MHC-Binding Characteristics of the 129/J Ly49 Repertoire J. Immunol., April 15, 2001; 166(8): 5034 - 5043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Y. Choi, Y. Yoshimura, G. J. Christianson, T. J. Sproule, S. Malarkannan, N. Shastri, S. Joyce, and D. C. Roopenian Quantitative Analysis of the Immune Response to Mouse Non-MHC Transplantation Antigens In Vivo: The H60 Histocompatibility Antigen Dominates Over All Others J. Immunol., April 1, 2001; 166(7): 4370 - 4379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Moser, J. D. Altman, and A. E. Lukacher Antiviral Cd8+ T Cell Responses in Neonatal Mice: Susceptibility to Polyoma Virus-Induced Tumors Is Associated with Lack of Cytotoxic Function by Viral Antigen-Specific T Cells J. Exp. Med., March 5, 2001; 193(5): 595 - 606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Welsh Assessing Cd8 T Cell Number and Dysfunction in the Presence of Antigen J. Exp. Med., March 5, 2001; 193(5): F19 - F22. [Full Text] [PDF]
|
|
|
|
|
|
D. R. Drake III, M. L. Shawver, A. Hadley, E. Butz, C. Maliszewski, and A. E. Lukacher Induction of Polyomavirus-Specific CD8+ T Lymphocytes by Distinct Dendritic Cell Subpopulations J. Virol., January 1, 2001; 75(1): 544 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Drake III, J. M. Moser, A. Hadley, J. D. Altman, C. Maliszewski, E. Butz, and A. E. Lukacher Polyomavirus-Infected Dendritic Cells Induce Antiviral CD8+ T Lymphocytes J. Virol., May 1, 2000; 74(9): 4093 - 4101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Dey, R. P. Bronson, J. Dahl, J. P. Carroll, and T. L. Benjamin Accelerated Development of Polyoma Tumors and Embryonic Lethality: Different Effects of p53 Loss on Related Mouse Backgrounds Cell Growth Differ., May 1, 2000; 11(5): 231 - 237. [Abstract] [Full Text]
|
|
|
|
|
|
P. Velupillai, I. Yoshizawa, D. C. Dey, S. R. Nahill, J. P. Carroll, R. T. Bronson, and T. L. Benjamin Wild-Derived Inbred Mice Have a Novel Basis of Susceptibility to Polyomavirus-Induced Tumors J. Virol., December 1, 1999; 73(12): 10079 - 10085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Lukacher, J. M. Moser, A. Hadley, and J. D. Altman Visualization of Polyoma Virus-Specific CD8+ T Cells In Vivo During Infection and Tumor Rejection J. Immunol., September 15, 1999; 163(6): 3369 - 3378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Wilson, J. M. Moser, J. D. Altman, P. E. Jensen, and A. E. Lukacher Cross-Recognition of Two Middle T Protein Epitopes by Immunodominant Polyoma Virus-Specific CTL J. Immunol., April 1, 1999; 162(7): 3933 - 3941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Carroll, J. S. Fung, R. T. Bronson, E. Razvi, and T. L. Benjamin Radiation-Resistant and Radiation-Sensitive Forms of Host Resistance to Polyomavirus J. Virol., February 1, 1999; 73(2): 1213 - 1218. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
