| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Knockout Mice1
Departments of
*
Oral Biology and
Microbiology, The Immunobiology Vaccine Center, University of Alabama at Birmingham, Medical Center, Birmingham, AL 35294; and
Department of Mucosal Immunology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
Splenic T cells isolated from BALB/c mice that had been mucosally
tolerized by oral administration of 25 mg of OVA revealed selective
increases in IFN-
production with impaired levels of IL-2, IL-4,
IL-5, and IL-10. These mice possessed reduced splenic OVA-specific T
cell proliferative and delayed-type hypersensitivity responses when
compared with nontolerized controls. Further, OVA-specific IgG Ab
responses in serum and the numbers of IgG Ab-forming cells in spleen
were significantly diminished following systemic challenge with OVA in
CFA. When IFN--deficient (IFN--/-) mice of
the same genetic background were given an oral dose of 25 mg of OVA
before systemic immunization, no reduction in OVA-specific IgG Ab
responses in serum and spleen was seen. Furthermore, the serum IgG Ab
responses were restricted to IgG1 and IgG2b subclasses. Interestingly,
although IFN--/- mice displayed a partial diminishment
of T cell proliferative and delayed-type hypersensitivity responses to
OVA, significant responses were still present when compared with the
low responses noted in IFN-+/+ mice. In addition,
OVA-specific T cells from IFN--/- mice produced
Th2-type cytokines (e.g., IL-4), which provided help for systemic
OVA-specific serum IgG1 and IgG2b Ab responses. These findings clearly
indicate a central role for IFN- in the induction and maintenance of
mucosally induced tolerance.
This article has been cited by other articles:
|
|
 |
|
  I. Dotan, M. Allez, A. Nakazawa, J. Brimnes, M. Schulder-Katz, and L. Mayer Intestinal epithelial cells from inflammatory bowel disease patients preferentially stimulate CD4+ T cells to proliferate and secrete interferon-{gamma} Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1630 - G1640. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Izawa, K. Sano, M. Takehara, M. Inobe, J.-i. Suzuki, H. Imamura, M. Takahashi, U. Ikeda, M. Isobe, and T. Uede Cre/loxP-mediated CTLA4IgG gene transfer induces clinically relevant immunosuppression via on-off gene recombination in vivo Cardiovasc Res, January 1, 2006; 69(1): 289 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Akadegawa, S. Ishikawa, T. Sato, J. Suzuki, H. Yurino, M. Kitabatake, T. Ito, T. Kuriyama, and K. Matsushima Breakdown of Mucosal Immunity in the Gut and Resultant Systemic Sensitization by Oral Antigens in a Murine Model for Systemic Lupus Erythematosus J. Immunol., May 1, 2005; 174(9): 5499 - 5506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hino, M.-N. Kweon, K. Fujihashi, J. R. McGhee, and H. Kiyono Pathological Role of Large Intestinal IL-12p40 for the Induction of Th2-Type Allergic Diarrhea Am. J. Pathol., April 1, 2004; 164(4): 1327 - 1335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Prioult, I. Fliss, and S. Pecquet Effect of Probiotic Bacteria on Induction and Maintenance of Oral Tolerance to {beta}-Lactoglobulin in Gnotobiotic Mice Clin. Vaccine Immunol., September 1, 2003; 10(5): 787 - 792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kato, K. Fujihashi, R. Kato, T. Dohi, K. Fujihashi, Y. Hagiwara, K. Kataoka, R. Kobayashi, and J. R. McGhee Lack of oral tolerance in aging is due to sequential loss of Peyer's patch cell interactions Int. Immunol., February 1, 2003; 15(2): 145 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Cobelens, A. Kavelaars, A. Vroon, M. Ringeling, R. van der Zee, W. van Eden, and C. J. Heijnen The {beta}2-Adrenergic Agonist Salbutamol Potentiates Oral Induction of Tolerance, Suppressing Adjuvant Arthritis and Antigen-Specific Immunity J. Immunol., November 1, 2002; 169(9): 5028 - 5035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Cobelens, A. Kavelaars, R. van der Zee, W. van Eden, and C. J. Heijnen Dynamics of mycobacterial HSP65-induced T-cell cytokine expression during oral tolerance induction in adjuvant arthritis Rheumatology, July 1, 2002; 41(7): 775 - 779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M-N Kweon, I Takahashi, M Yamamoto, M H Jang, N Suenobu, and H Kiyono Development of antigen induced colitis in SCID mice reconstituted with spleen derived memory type CD4+ CD45RB+ T cells Gut, March 1, 2002; 50(3): 299 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. George-Chandy, K. Eriksson, M. Lebens, I. Nordstrom, E. Schon, and J. Holmgren Cholera Toxin B Subunit as a Carrier Molecule Promotes Antigen Presentation and Increases CD40 and CD86 Expression on Antigen-Presenting Cells Infect. Immun., September 1, 2001; 69(9): 5716 - 5725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Kahn, D. C. Archer, and C. J. Kelly Absence of Functional Inducible NO Synthase Enhances the Efficacy of Tolerance Induced by High Dose Antigen Feeding J. Immunol., December 1, 2000; 165(11): 6116 - 6122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yamamoto, P. Rennert, J. R. McGhee, M.-N. Kweon, S. Yamamoto, T. Dohi, S. Otake, H. Bluethmann, K. Fujihashi, and H. Kiyono Alternate Mucosal Immune System: Organized Peyer's Patches Are Not Required for IgA Responses in the Gastrointestinal Tract J. Immunol., May 15, 2000; 164(10): 5184 - 5191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Fujihashi, T. Dohi, M.-N. Kweon, J. R. Mcghee, T. Koga, M. D. Cooper, S. Tonegawa, and H. Kiyono {gamma}{delta}T cells regulate mucosally induced tolerance in a dose-dependent fashion Int. Immunol., December 1, 1999; 11(12): 1907 - 1916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. McI. Mowat, M. Steel, A. J. Leishman, and P. Garside Normal Induction of Oral Tolerance in the Absence of a Functional IL-12-Dependent IFN-{gamma} Signaling Pathway J. Immunol., November 1, 1999; 163(9): 4728 - 4736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Iijima, I. Takahashi, D. Kishi, J.-K. Kim, S. Kawano, M. Hori, and H. Kiyono Alteration of Interleukin 4 Production Results in the Inhibition of T Helper Type 2 Cell–dominated Inflammatory Bowel Disease in T Cell Receptor {alpha} Chain–deficient Mice J. Exp. Med., September 6, 1999; 190(5): 607 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. N. Shi, M. J. Grusby, and C. Nagler-Anderson Orally Induced Peripheral Nonresponsiveness Is Maintained in the Absence of Functional Th1 or Th2 Cells J. Immunol., May 1, 1999; 162(9): 5143 - 5148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-N. Kweon, K. Fujihashi, Y. Wakatsuki, T. Koga, M. Yamamoto, J. R. McGhee, and H. Kiyono Mucosally Induced Systemic T Cell Unresponsiveness to Ovalbumin Requires CD40 Ligand-CD40 Interactions J. Immunol., February 15, 1999; 162(4): 1904 - 1909. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. ROSSINI, D. L. GREINER, and J. P. MORDES Induction of Immunologic Tolerance for Transplantation Physiol Rev, January 1, 1999; 79(1): 99 - 141. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
