| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,#,¶
,¶,#,¶,§
*
The Nora Eccles Harrison Cardiovascular Research and Training Institute,
The Eccles Program in Human Molecular Biology and Genetics, and the Departments of
Anatomy,
§
Biochemistry,
¶
Medicine,
||
Pathology, and
#
Pediatrics, University of Utah School of Medicine, Salt Lake City, UT 84112
The synthesis of prostanoids is regulated by cyclooxygenases
(prostaglandin H synthases), which catalyze the conversion of
arachidonic acid to PGH2. Cyclooxygenases are the target of
aspirin and other nonsteroidal anti-inflammatory agents. In this
study, we found that human polymorphonuclear leukocytes (PMNs) express
the inducible isoform of cyclooxygenase, COX-2, when stimulated by LPS
whereas the protein was not detectable in freshly isolated human PMNs.
We also found by immunohistochemical analysis that COX-2 is expressed
in PMNs in inflamed human tissues. COX-2 was induced in a time- and
concentration-dependent fashion when isolated human PMNs were exposed
to LPS; COX-2 was also induced, or its expression was increased, by
TNF-
, IL-1, and IL-8. Expression of COX-2 in stimulated PMNs was
paralleled by secretion of PGE2. The release of
PGE2 was blocked by a selective nonsteroidal inhibitor of
COX-2, indicating that the enzyme is responsible for the prostanoids
produced, and was inhibited by dexamethasone. The time course of
LPS-induced COX-2 expression and other features were different in
freshly isolated PMNs, monocytes, and macrophages, indicating that
COX-2 expression is differentially regulated in myeloid cells of
different lineages and degrees of maturation. Consistent with this,
IL-4 and IL-10, which suppressed LPS-induced COX-2 expression in
monocytes, had little effect on this response by PMNs. These
experiments demonstrate that PMNs express COX-2 when appropriately
stimulated. Thus, they may actively influence the eicosanoid
composition of the acute inflammatory milieu.
This article has been cited by other articles:
|
|
 |
|
  S. Negrotto, E. Malaver, M. E. Alvarez, N. Pacienza, L. P. D'Atri, R. G. Pozner, R. M. Gomez, and M. Schattner Aspirin and Salicylate Suppress Polymorphonuclear Apoptosis Delay Mediated by Proinflammatory Stimuli J. Pharmacol. Exp. Ther., November 1, 2006; 319(2): 972 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Vega, P. Chacon, G. Alba, R. El Bekay, J. Monteseirin, J. Martin-Nieto, and F. Sobrino Modulation of IgE-dependent COX-2 gene expression by reactive oxygen species in human neutrophils J. Leukoc. Biol., July 1, 2006; 80(1): 152 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wang, A. Bergh, and J.-E. Damber Cyclooxygenase-2 Expression Correlates with Local Chronic Inflammation and Tumor Neovascularization in Human Prostate Cancer Clin. Cancer Res., May 1, 2005; 11(9): 3250 - 3256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-S. Cadieux, P. Leclerc, M. St-Onge, A.-A. Dussault, C. Laflamme, S. Picard, C. Ledent, P. Borgeat, and M. Pouliot Potentiation of neutrophil cyclooxygenase-2 by adenosine: an early anti-inflammatory signal J. Cell Sci., April 1, 2005; 118(7): 1437 - 1447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-S. Cho and C. Chae Expression of Nitric Oxide Synthase 2 and Cyclooxygenase-2 in Swine Experimentally Infected with Actinobacillus pleuropneumoniae Vet. Pathol., November 1, 2004; 41(6): 666 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. F. Liberty, L. Raichel, Z. Hazan-Eitan, I. Pessach, N. Hadad, F. Schlaeffer, and R. Levy Cytosolic phospholipase A2 is responsible for prostaglandin E2 and leukotriene B4 formation in phagocyte-like PLB-985 cells: studies of differentiated cPLA2-deficient PLB-985 cells J. Leukoc. Biol., July 1, 2004; 76(1): 176 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Trouba and D. R. Germolec Micromolar Concentrations of Sodium Arsenite Induce Cyclooxygenase-2 Expression and Stimulate p42/44 Mitogen-Activated Protein Kinase Phosphorylation in Normal Human Epidermal Keratinocytes Toxicol. Sci., June 1, 2004; 79(2): 248 - 257. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Lindemann, C. C. Yost, M. M. Denis, T. M. McIntyre, A. S. Weyrich, and G. A. Zimmerman Neutrophils alter the inflammatory milieu by signal-dependent translation of constitutive messenger RNAs PNAS, May 4, 2004; 101(18): 7076 - 7081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Cipollone, B. Rocca, and C. Patrono Cyclooxygenase-2 Expression and Inhibition in Atherothrombosis Arterioscler. Thromb. Vasc. Biol., February 1, 2004; 24(2): 246 - 255. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. F.J. de Vries, A. van Waarde, A. R. Buursma, and W. Vaalburg Synthesis and In Vivo Evaluation of 18F-Desbromo-DuP-697 as a PET Tracer for Cyclooxygenase-2 Expression J. Nucl. Med., October 1, 2003; 44(10): 1700 - 1706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-S. Cho and C. Chae Expression of Cyclooxygenase-2 in Swine Naturally Infected with Actinobacillus pleuropneumoniae Vet. Pathol., January 1, 2003; 40(1): 25 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Chen, H. He, E. F. Mondejar, and G. Hedenstierna Cyclooxygenase inhibitor blocks rebound response after NO inhalation in an endotoxin model Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H290 - H298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. Podolin, B. J. Bolognese, J. J. Foley, D. B. Schmidt, P. T. Buckley, K. L. Widdowson, Q. Jin, J. R. White, J. M. Lee, R. B. Goodman, et al. A Potent and Selective Nonpeptide Antagonist of CXCR2 Inhibits Acute and Chronic Models of Arthritis in the Rabbit J. Immunol., December 1, 2002; 169(11): 6435 - 6444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Clark, M. J. Coffey, R. M. Maclean, P. W. Collins, M. J. Lewis, A. R. Cross, and V. B. O'Donnell Characterization of Nitric Oxide Consumption Pathways by Normal, Chronic Granulomatous Disease and Myeloperoxidase-Deficient Human Neutrophils J. Immunol., November 15, 2002; 169(10): 5889 - 5896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pouliot, M.-E. Fiset, M. Masse, P. H. Naccache, and P. Borgeat Adenosine Up-Regulates Cyclooxygenase-2 in Human Granulocytes: Impact on the Balance of Eicosanoid Generation J. Immunol., November 1, 2002; 169(9): 5279 - 5286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Murakami, D. Takahashi, T. Kinoshita, K. Koshimizu, H. W. Kim, A. Yoshihiro, Y. Nakamura, S. Jiwajinda, J. Terao, and H. Ohigashi Zerumbone, a Southeast Asian ginger sesquiterpene, markedly suppresses free radical generation, proinflammatory protein production, and cancer cell proliferation accompanied by apoptosis: the {alpha},{beta}-unsaturated carbonyl group is a prerequisite Carcinogenesis, May 1, 2002; 23(5): 795 - 802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Whittaker, K. S. Bahjat, L. L. Moldawer, and M. J. Clare-Salzler Autoregulation of Human Monocyte-Derived Dendritic Cell Maturation and IL-12 Production by Cyclooxygenase- 2-Mediated Prostanoid Production J. Immunol., October 15, 2000; 165(8): 4298 - 4304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Murakami, Y. Nakamura, T. Tanaka, K. Kawabata, D. Takahashi, K. Koshimizu, and H. Ohigashi Suppression by citrus auraptene of phorbol ester- and endotoxin-induced inflammatory responses: role of attenuation of leukocyte activation Carcinogenesis, October 1, 2000; 21(10): 1843 - 1850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Notoya, D. V. Jovanovic, P. Reboul, J. Martel-Pelletier, F. Mineau, and J.-P. Pelletier The Induction of Cell Death in Human Osteoarthritis Chondrocytes by Nitric Oxide Is Related to the Production of Prostaglandin E2 Via the Induction of Cyclooxygenase-2 J. Immunol., September 15, 2000; 165(6): 3402 - 3410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. F. Pereira, L. A. Boven, J. Middel, J. Verhoef, and H. S. L. M. Nottet Induction of cyclooxygenase-2 expression during HIV-1-infected monocyte-derived macrophage and human brain microvascular endothelial cell interactions J. Leukoc. Biol., September 1, 2000; 68(3): 423 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. N. M. Khan, K. Stanfield, D. Trajkovic, and R. K. Harris Cyclooxygenase-2 Expression in Inflammatory Lung Lesions of Nonhuman Primates Vet. Pathol., September 1, 2000; 37(5): 512 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yang, D. Sun, Y. G. Huang, A. Smart, J. P. Briggs, and J. B. Schnermann Differential regulation of COX-2 expression in the kidney by lipopolysaccharide: role of CD14 Am J Physiol Renal Physiol, July 1, 1999; 277(1): F10 - F16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Czermak, V. Sarma, N. M. Bless, H. Schmal, H. P. Friedl, and P. A. Ward In Vitro and In Vivo Dependency of Chemokine Generation on C5a and TNF-{alpha} J. Immunol., February 15, 1999; 162(4): 2321 - 2325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Pillinger, C. Capodici, P. Rosenthal, N. Kheterpal, S. Hanft, M. R. Philips, and G. Weissmann Modes of action of aspirin-like drugs: Salicylates inhibit Erk activation and integrin-dependent neutrophil adhesion PNAS, November 24, 1998; 95(24): 14540 - 14545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pouliot, C. Gilbert, P. Borgeat, P. E. Poubelle, S. Bourgoin, C. Créminon, J. Maclouf, S. R. Mccoll, and P. H. Naccache Expression and activity of prostaglandin endoperoxide synthase-2 in agonist-activated human neutrophils FASEB J, September 1, 1998; 12(12): 1109 - 1123. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
