HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Handzel, Z. T. Articles by Gern, J. E. Search for Related Content PubMed PubMed Citation Articles by Handzel, Z. T. Articles by Gern, J. E.

Eosinophils Bind Rhinovirus and Activate Virus-Specific T Cells¹

Zeev T. Handzel^2,*, William W. Busse^*, Julie B. Sedgwick^*, Rose Vrtis^*, Wai Ming Lee, E. A. B. Kelly^* and James E. Gern^3,

Departments of ^* Medicine, Molecular Virology, and Pediatrics, University of Wisconsin, Madison, WI 53792

Episodes of virus-induced exacerbations of asthma are accompanied by increased eosinophils (EOS) in respiratory secretions and evidence of EOS degranulation. Although rhinoviruses (RV) are the viruses most often implicated in exacerbations of asthma in both children and adults, little is known about the immune response to this group of viruses and, in particular, EOS-RV interactions. To define such interactions, we incubated human rhinovirus type 16 (RV16), a serotype using ICAM-1 as a receptor, with EOS purified from PBMC, and measured EOS-RV binding, EOS-mediated Ag presentation and T cell activation, and EOS cell surface marker expression and superoxide production. Significant RV16 binding occurred to EOS that were pretreated with granulocyte-macrophage CSF, and this binding was inhibited by anti-ICAM-1 mAb. EOS also presented viral Ags to RV16-specific T cells, causing T cell proliferation and secretion of IFN-. RV16 induced a significant shift from CD18^dim to CD18^bright, but did not affect EOS expression of CD54, CD69, or HLA-DR. Finally, RV16 did not induce superoxide production from peripheral blood EOS. These findings suggest that RV16 also binds to airway EOS, which resemble granulocyte-macrophage CSF-treated blood EOS in terms of high expression of ICAM-1. Furthermore, our findings suggest that EOS could participate in RV-induced immune responses through Ag presentation and T cell activation. By activating RV-specific T cells, EOS may play an important role in the initiation of antiviral T cell responses, and these effects could also contribute to enhanced airway inflammation and increased asthma symptoms in susceptible individuals.

This article has been cited by other articles:

				U. M. Padigel, J. J. Lee, T. J. Nolan, G. A. Schad, and D. Abraham Eosinophils Can Function as Antigen-Presenting Cells To Induce Primary and Secondary Immune Responses to Strongyloides stercoralis. Infect. Immun., June 1, 2006; 74(6): 3232 - 3238. [Abstract] [Full Text] [PDF]

				H.-Z. Shi Eosinophils function as antigen-presenting cells J. Leukoc. Biol., September 1, 2004; 76(3): 520 - 527. [Abstract] [Full Text] [PDF]

				S. D. Message and S. L. Johnston Host defense function of the airway epithelium in health and disease: clinical background J. Leukoc. Biol., January 1, 2004; 75(1): 5 - 17. [Abstract] [Full Text] [PDF]

				J. de Kluijver, C. E. Evertse, J. K. Sont, J. A. Schrumpf, C. J. G. van Zeijl-van der Ham, C. R. Dick, K. F. Rabe, P. S. Hiemstra, and P. J. Sterk Are Rhinovirus-induced Airway Responses in Asthma Aggravated by Chronic Allergen Exposure? Am. J. Respir. Crit. Care Med., November 15, 2003; 168(10): 1174 - 1180. [Abstract] [Full Text] [PDF]

				H. Nagase, S. Okugawa, Y. Ota, M. Yamaguchi, H. Tomizawa, K. Matsushima, K. Ohta, K. Yamamoto, and K. Hirai Expression and Function of Toll-Like Receptors in Eosinophils: Activation by Toll-Like Receptor 7 Ligand J. Immunol., October 15, 2003; 171(8): 3977 - 3982. [Abstract] [Full Text] [PDF]

				D. J. Adamko, A. D. Fryer, B. S. Bochner, and D. B. Jacoby CD8+ T Lymphocytes in Viral Hyperreactivity and M2 Muscarinic Receptor Dysfunction Am. J. Respir. Crit. Care Med., February 15, 2003; 167(4): 550 - 556. [Abstract] [Full Text] [PDF]

				D. J. Adamko, B. L. Yost, G. J. Gleich, A. D. Fryer, and D. B. Jacoby Ovalbumin Sensitization Changes the Inflammatory Response to Subsequent Parainfluenza Infection: Eosinophils Mediate Airway Hyperresponsiveness, M2 Muscarinic Receptor Dysfunction, and Antiviral Effects J. Exp. Med., November 15, 1999; 190(10): 1465 - 1478. [Abstract] [Full Text] [PDF]

				M. A. Giembycz and M. A. Lindsay Pharmacology of the Eosinophil Pharmacol. Rev., June 1, 1999; 51(2): 213 - 340. [Abstract] [Full Text] [PDF]

				J. E. Gern and W. W. Busse Association of Rhinovirus Infections with Asthma Clin. Microbiol. Rev., January 1, 1999; 12(1): 9 - 18. [Abstract] [Full Text] [PDF]