| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Departments of Microbiology and
Department of Comparative Medicine, University of Alabama, Birmingham, AL 35294; and
School of Microbiology and Immunology, University of New South Wales, Sydney, New South Wales, Australia
Secretory IgA (S-IgA), a major humoral mediator of mucosal immunity, is a polymeric Ig containing an unusual extra polypeptide, secretory component (SC), added during transcytosis through epithelial cells. Polymeric S-IgA is more effective than monomeric IgA (mIgA) and IgG in neutralizing viruses. It is not known whether this increased efficacy is due solely to the polymeric structure of the molecule or whether SC itself makes S-IgA more efficient; a quantitative in vitro comparison of the biologic activities of S-IgA and pIgA has not been reported. We prepared purified pIgA and mIgA mAbs directed toward the H1 hemagglutinin of PR8 influenza virus and purified monoclonal S-IgA (made from monoclonal pIgA injected into a Lewis rat and collected as S-IgA from bile) and compared their abilities to carry out hemagglutination inhibition (HI) and neutralization of the infectivity of PR8 influenza virus in vitro. The polymeric Igs (pIgA and S-IgA) were 5 times more effective than mIgA in HI and 7 to 10 times more effective than mIgA in virus neutralization. Addition of SC to pIgA did not modify its ability to mediate HI and had only a minimal effect (S-IgA was 1.4 times more effective) on its ability to neutralize influenza virus in vitro. Trypsin preincubation partially abolished mIgA- or pIgA-mediated, but not S-IgA-mediated, viral neutralization. Thus, although S-IgA is more stable functionally than pIgA, the addition of SC does not influence, positively or negatively, the biologic activity associated with the Fab of S-IgA.
This article has been cited by other articles:
|
|
 |
|
  L. Baudino, L. Fossati-Jimack, C. Chevalley, E. Martinez-Soria, M. J. Shulman, and S. Izui IgM and IgA anti-erythrocyte autoantibodies induce anemia in a mouse model through multivalency-dependent hemagglutination but not through complement activation Blood, June 15, 2007; 109(12): 5355 - 5362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Fasching, T. Grossman, B. Corthesy, A. G. Plaut, J. N. Weiser, and E. N. Janoff Impact of the Molecular Form of Immunoglobulin A on Functional Activity in Defense against Streptococcus pneumoniae Infect. Immun., April 1, 2007; 75(4): 1801 - 1810. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Moldoveanu, W.-Q. Huang, R. Kulhavy, M. S. Pate, and J. Mestecky Human Male Genital Tract Secretions: Both Mucosal and Systemic Immune Compartments Contribute to the Humoral Immunity J. Immunol., September 15, 2005; 175(6): 4127 - 4136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yoshikawa, K. Matsuo, K. Matsuo, Y. Suzuki, A. Nomoto, S.-i. Tamura, T. Kurata, and T. Sata Total viral genome copies and virus-Ig complexes after infection with influenza virus in the nasal secretions of immunized mice J. Gen. Virol., August 1, 2004; 85(8): 2339 - 2346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. B. Renegar, P. A. Small Jr, L. G. Boykins, and P. F. Wright Role of IgA versus IgG in the Control of Influenza Viral Infection in the Murine Respiratory Tract J. Immunol., August 1, 2004; 173(3): 1978 - 1986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wolbank, R. Kunert, G. Stiegler, and H. Katinger Characterization of Human Class-Switched Polymeric (Immunoglobulin M [IgM] and IgA) Anti-Human Immunodeficiency Virus Type 1 Antibodies 2F5 and 2G12 J. Virol., April 1, 2003; 77(7): 4095 - 4103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. van Spriel, J. H. W. Leusen, H. Vile, and J. G. J. van de Winkel Mac-1 (CD11b/CD18) as Accessory Molecule for Fc{alpha}R (CD89) Binding of IgA J. Immunol., October 1, 2002; 169(7): 3831 - 3836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Schwartz-Cornil, Y. Benureau, H. Greenberg, B. A. Hendrickson, and J. Cohen Heterologous Protection Induced by the Inner Capsid Proteins of Rotavirus Requires Transcytosis of Mucosal Immunoglobulins J. Virol., July 17, 2002; 76(16): 8110 - 8117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Asahi, T. Yoshikawa, I. Watanabe, T. Iwasaki, H. Hasegawa, Y. Sato, S.-i. Shimada, M. Nanno, Y. Matsuoka, M. Ohwaki, et al. Protection Against Influenza Virus Infection in Polymeric Ig Receptor Knockout Mice Immunized Intranasally with Adjuvant-Combined Vaccines J. Immunol., March 15, 2002; 168(6): 2930 - 2938. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-E. Johansen, R. Braathen, and P. Brandtzaeg The J Chain Is Essential for Polymeric Ig Receptor-Mediated Epithelial Transport of IgA J. Immunol., November 1, 2001; 167(9): 5185 - 5192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Roos, L. H. Bouwman, D. J. van Gijlswijk-Janssen, M. C. Faber-Krol, G. L. Stahl, and M. R. Daha Human IgA Activates the Complement System Via the Mannan-Binding Lectin Pathway J. Immunol., September 1, 2001; 167(5): 2861 - 2868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.J. PADULA, C.M. ROSSI, M.O. D. VALLE, P.V. MARTINEZ, S.B. COLAVECCHIA, A. EDELSTEIN, S.D. L. MIGUEL, R.D. RABINOVICH, and E.L. SEGURA Development and evaluation of a solid-phase enzyme immunoassay based on Andes hantavirus recombinant nucleoprotein J. Med. Microbiol., February 1, 2000; 49(2): 149 - 155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Roe, I. N. Norderhaug, P. Brandtzaeg, and F.-E. Johansen Fine Specificity of Ligand-Binding Domain 1 in the Polymeric Ig Receptor: Importance of the CDR2-Containing Region for IgM Interaction J. Immunol., May 15, 1999; 162(10): 6046 - 6052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J MESTECKY, M W RUSSELL, and C O ELSON Intestinal IgA: novel views on its function in the defence of the largest mucosal surface Gut, January 1, 1999; 44(1): 2 - 5. [Full Text] [PDF]
|
|
|
|
|
|
P. Crottet and B. Corthesy Secretory Component Delays the Conversion of Secretory IgA into Antigen-Binding Competent F(ab')2: A Possible Implication for Mucosal Defense J. Immunol., November 15, 1998; 161(10): 5445 - 5453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Motegi and H. Kita Interaction with Secretory Component Stimulates Effector Functions of Human Eosinophils But Not of Neutrophils J. Immunol., October 15, 1998; 161(8): 4340 - 4346. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
