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Laboratory of Experimental Immunology, Division of Basic Sciences, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD 21702
The Ly-49 gene families are class I-recognizing receptors on murine
NK cells. Most Ly-49 receptors inhibit NK cell lysis upon recognizing
their target class I ligands. In this report we have examined the
ability of Ly-49A and Ly-49G2 to regulate T cell functions on
CD3+ cells, primarily the subset that also expresses
NK-1.1 and/or DX5. The majority (>50%) of T cells that express Ly-49
molecules also coexpress NK-1.1 and/or DX5, although some
NK-1.1- and/or DX5-/CD3+ cells
express Ly-49 molecules. Lysis of target cells by IL-2-cultured T cells
expressing Ly-49A and G2 was enhanced by Abs specific for Ly-49A and G2
as well as by Abs to class I (H-2Dd 
1/2). Murine T
cells also were cultured in the presence of targets that express
(H-2Dd) which is inhibiting for the Ly-49A and G2
receptors. These cells were examined for a coincident increase in
cytokine production (IFN-
, TNF-, and granulocyte-macrophage CSF).
Abs to Ly-49A and G2 or their respective class I ligands blocked the
negative signals mediated via the Ly-49 receptors and increased IFN-
and granulocyte-macrophage CSF production after interaction of these T
cells with H-2Dd-expressing tumor targets. Furthermore, an
EL-4 T cell line expressing both Ly-49A and G2, when treated with mAb
YE148 and 4D11, demonstrated reduced cytokine production and calcium
mobilization. These results demonstrate for the first time that Ly-49
class I binding receptors, previously thought to be restricted to mouse
NK cells, can mediate important physiological functions of T cell
subsets.
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J. R. Ortaldo, E. W. Bere, D. Hodge, and H. A. Young Activating Ly-49 NK Receptors: Central Role in Cytokine and Chemokine Production J. Immunol., April 15, 2001; 166(8): 4994 - 4999. [Abstract] [Full Text] [PDF]
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K. Van Beneden, F. Stevenaert, A. De Creus, V. Debacker, J. De Boever, J. Plum, and G. Leclercq Expression of Ly49E and CD94/NKG2 on Fetal and Adult NK Cells J. Immunol., April 1, 2001; 166(7): 4302 - 4311. [Abstract] [Full Text] [PDF]
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T. Hanke and D. H. Raulet Cumulative Inhibition of NK Cells and T Cells Resulting from Engagement of Multiple Inhibitory Ly49 Receptors J. Immunol., March 1, 2001; 166(5): 3002 - 3007. [Abstract] [Full Text] [PDF]
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J. R. Ortaldo, R. Winkler-Pickett, and G. Wiegand Activating Ly-49D NK receptors: expression and function in relation to ontogeny and Ly-49 inhibitor receptors J. Leukoc. Biol., November 1, 2000; 68(5): 748 - 756. [Abstract] [Full Text]
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E. Assarsson, T. Kambayashi, J. K. Sandberg, S. Hong, M. Taniguchi, L. Van Kaer, H.-G. Ljunggren, and B. J. Chambers CD8+ T Cells Rapidly Acquire NK1.1 and NK Cell-Associated Molecules Upon Stimulation In Vitro and In Vivo J. Immunol., October 1, 2000; 165(7): 3673 - 3679. [Abstract] [Full Text] [PDF]
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E. T. Silver, D.-E. Gong, C. S. Chang, A. Amrani, P. Santamaria, and K. P. Kane Ly-49P Activates NK-Mediated Lysis by Recognizing H-2Dd 1 J. Immunol., August 15, 2000; 165(4): 1771 - 1781. [Abstract] [Full Text] [PDF]
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P. Brawand, F. A. Lemonnier, H. R. MacDonald, J.-C. Cerottini, and W. Held Transgenic Expression of Ly49A on T Cells Impairs a Specific Antitumor Response J. Immunol., August 15, 2000; 165(4): 1871 - 1876. [Abstract] [Full Text] [PDF]
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C. D. Peacock, M. Y. Lin, J. R. Ortaldo, and R. M. Welsh The Virus-Specific and Allospecific Cytotoxic T-Lymphocyte Response to Lymphocytic Choriomeningitis Virus Is Modified in a Subpopulation of CD8+ T Cells Coexpressing the Inhibitory Major Histocompatibility Complex Class I Receptor Ly49G2 J. Virol., August 1, 2000; 74(15): 7032 - 7038. [Abstract] [Full Text]
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F. Gays, M. Unnikrishnan, S. Shrestha, K. P. Fraser, A. R. Brown, C. M. G. Tristram, Z. M. A. Chrzanowska-Lightowlers, and C. G. Brooks The Mouse Tumor Cell Lines EL4 and RMA Display Mosaic Expression of NK-Related and Certain Other Surface Molecules and Appear to Have a Common Origin J. Immunol., May 15, 2000; 164(10): 5094 - 5102. [Abstract] [Full Text] [PDF]
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H. R.C. Smith, H. H. Chuang, L. L. Wang, M. Salcedo, J. W. Heusel, and W. M. Yokoyama Nonstochastic Coexpression of Activation Receptors on Murine Natural Killer Cells J. Exp. Med., April 18, 2000; 191(8): 1341 - 1354. [Abstract] [Full Text] [PDF]
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M. K. Slifka, R. R. Pagarigan, and J. L. Whitton NK Markers Are Expressed on a High Percentage of Virus-Specific CD8+ and CD4+ T Cells J. Immunol., February 15, 2000; 164(4): 2009 - 2015. [Abstract] [Full Text] [PDF]
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L. Fahlen, L. Oberg, T. Brannstrom, N. K. S. Khoo, U. Lendahl, and C. L. Sentman Ly49A expression on T cells alters T cell selection Int. Immunol., February 1, 2000; 12(2): 215 - 222. [Abstract] [Full Text] [PDF]
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S. Pied, J. Roland, A. Louise, D. Voegtle, V. Soulard, D. Mazier, and P.-A. Cazenave Liver CD4-CD8- NK1.1+ TCR{alpha}{beta} Intermediate Cells Increase During Experimental Malaria Infection and Are Able to Exhibit Inhibitory Activity Against the Parasite Liver Stage In Vitro J. Immunol., February 1, 2000; 164(3): 1463 - 1469. [Abstract] [Full Text] [PDF]
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L. H. Mason, J. Willette-Brown, A. T. Mason, D. McVicar, and J. R. Ortaldo Interaction of Ly-49D+ NK Cells with H-2Dd Target Cells Leads to Dap-12 Phosphorylation and IFN-{gamma} Secretion J. Immunol., January 15, 2000; 164(2): 603 - 611. [Abstract] [Full Text] [PDF]
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M. Pauza, K. M. Smith, H. Neal, C. Reilly, L. L. Lanier, and D. Lo Transgenic Expression of Ly-49A in Thymocytes Alters Repertoire Selection J. Immunol., January 15, 2000; 164(2): 884 - 892. [Abstract] [Full Text] [PDF]
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J. R. Ortaldo, R. Winkler-Pickett, J. Willette-Brown, R. L. Wange, S. K. Anderson, G. J. Palumbo, L. H. Mason, and D. W. McVicar Structure/Function Relationship of Activating Ly-49D and Inhibitory Ly-49G2 NK Receptors ,2 J. Immunol., November 15, 1999; 163(10): 5269 - 5277. [Abstract] [Full Text] [PDF]
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A. J. Zajac, R. E. Vance, W. Held, D. J. D. Sourdive, J. D. Altman, D. H. Raulet, and R. Ahmed Impaired Anti-Viral T Cell Responses Due to Expression of the LY49A Inhibitory Receptor J. Immunol., November 15, 1999; 163(10): 5526 - 5534. [Abstract] [Full Text] [PDF]
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A. M. Moodycliffe, S. Maiti, and S. E. Ullrich Splenic NK1.1-Negative, TCR{alpha}{beta} Intermediate CD4+ T Cells Exist in Naive NK1.1 Allelic Positive and Negative Mice, with the Capacity to Rapidly Secrete Large Amounts of IL-4 and IFN-{gamma} Upon Primary TCR Stimulation J. Immunol., May 1, 1999; 162(9): 5156 - 5163. [Abstract] [Full Text] [PDF]
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 I. Apostolou, Y. Takahama, C. Belmant, T. Kawano, M. Huerre, G. Marchal, J. Cui, M. Taniguchi, H. Nakauchi, J.-J. Fournie, et al. Murine natural killer cells contribute to the granulomatous reaction caused by mycobacterial cell walls PNAS, April 27, 1999; 96(9): 5141 - 5146. [Abstract] [Full Text] [PDF]
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C. S. Chang, L. Brossay, M. Kronenberg, and K. P. Kane The Murine Nonclassical Class I Major Histocompatibility Complex-like CD1.1 Molecule Protects Target Cells from Lymphokine-activated Killer Cell Cytolysis J. Exp. Med., February 1, 1999; 189(3): 483 - 491. [Abstract] [Full Text] [PDF]
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N. Seo, Y. Tokura, F. Furukawa, and M. Takigawa Down-Regulation of Tumoricidal NK and NK T Cell Activities by MHC Kb Molecules Expressed on Th2-Type {gamma}{delta} T and {alpha}{beta} T Cells Coinfiltrating in Early B16 Melanoma Lesions J. Immunol., October 15, 1998; 161(8): 4138 - 4145. [Abstract] [Full Text] [PDF]
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H. Robson MacDonald, R. K. Lees, and W. Held Developmentally Regulated Extinction of Ly-49 Receptor Expression Permits Maturation and Selection of NK1.1+ T Cells J. Exp. Med., June 15, 1998; 187(12): 2109 - 2114. [Abstract] [Full Text] [PDF]
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