HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vrtala, S. Articles by Valenta, R. Search for Related Content PubMed PubMed Citation Articles by Vrtala, S. Articles by Valenta, R.

Immunization with Purified Natural and Recombinant Allergens Induces Mouse IgG1 Antibodies That Recognize Similar Epitopes as Human IgE and Inhibit the Human IgE-Allergen Interaction and Allergen-Induced Basophil Degranulation¹

Susanne Vrtala^*, Tanja Ball^*, Susanne Spitzauer, Budhi Pandjaitan, Cenk Suphioglu^§, Bruce Knox^§, Wolfgang R. Sperr, Peter Valent, Dietrich Kraft^* and Rudolf Valenta^2,*

Institutes of ^* General and Experimental Pathology and Medical and Clinical Chemistry, and Division of Hematology, Department of Internal Medicine I, AKH (Vienna General Hospital), University of Vienna, Austria; and ^§ Pollen and Allergen Research Group, School of Botany, University of Melbourne, Melbourne, Australia

Molecular characterization of allergens by recombinant DNA technology has made rapid progress in the recent few years. In the present study we immunized mice with aluminum hydroxide-adsorbed purified recombinant major timothy grass pollen allergens (rPhl p 1, rPhl p 2, rPhl p 5), dog albumin, a major animal dander allergen, and proteins with low (ß-lactoglobulin) or no (ribulose diphosphate carboxylase) allergenic potential in humans. Allergens that bind high levels of IgE in humans (Phl p 1, Phl p 5, dog albumin) induced high IgE and IgG1 levels in mice, whereas proteins with little or no allergenic activity in humans failed to induce significant IgE and IgG1 levels in mice. Continuous immunization for a period of 27 wk resulted in the production of mouse IgG1 Abs that recognized recombinant allergen fragments/epitopes defined by IgE Abs of allergic patients. As a consequence, allergen-specific mouse Abs strongly inhibited human IgE binding to the allergens and suppressed the allergen-induced histamine release from human basophils. In summary, our data indicate that 1) the allergenic potency of a protein may be related to its overall immunogenicity and 2) prolonged immunization with single purified recombinant allergens induces protective IgG Abs. The presented experimental in vivo/in vitro system allows the evaluation of Ag preparations (e.g., recombinant allergens) to be used for immunotherapy in humans.

This article has been cited by other articles:

				J. Edlmayr, K. Niespodziana, B. Linhart, M. Focke-Tejkl, K. Westritschnig, S. Scheiblhofer, A. Stoecklinger, M. Kneidinger, P. Valent, R. Campana, et al. A Combination Vaccine for Allergy and Rhinovirus Infections Based on Rhinovirus-Derived Surface Protein VP1 and a Nonallergenic Peptide of the Major Timothy Grass Pollen Allergen Phl p 1 J. Immunol., May 15, 2009; 182(10): 6298 - 6306. [Abstract] [Full Text] [PDF]

				N. Mothes-Luksch, S. Stumvoll, B. Linhart, M. Focke, M.-T. Krauth, A. Hauswirth, P. Valent, P. Verdino, T. Pavkov, W. Keller, et al. Disruption of Allergenic Activity of the Major Grass Pollen Allergen Phl p 2 by Reassembly as a Mosaic Protein J. Immunol., October 1, 2008; 181(7): 4864 - 4873. [Abstract] [Full Text] [PDF]

				U. Baranyi, B. Linhart, N. Pilat, M. Gattringer, J. Bagley, F. Muehlbacher, J. Iacomini, R. Valenta, and T. Wekerle Tolerization of a Type I Allergic Immune Response through Transplantation of Genetically Modified Hematopoietic Stem Cells J. Immunol., June 15, 2008; 180(12): 8168 - 8175. [Abstract] [Full Text] [PDF]

				K. Westritschnig, B. Linhart, M. Focke-Tejkl, T. Pavkov, W. Keller, T. Ball, A. Mari, A. Hartl, A. Stocklinger, S. Scheiblhofer, et al. A Hypoallergenic Vaccine Obtained by Tail-to-Head Restructuring of Timothy Grass Pollen Profilin, Phl p 12, for the Treatment of Cross-Sensitization to Profilin J. Immunol., December 1, 2007; 179(11): 7624 - 7634. [Abstract] [Full Text] [PDF]

				S. Vrtala, M. Focke, J. Kopec, P. Verdino, A. Hartl, W. R. Sperr, A. A. Fedorov, T. Ball, S. Almo, P. Valent, et al. Genetic Engineering of the Major Timothy Grass Pollen Allergen, Phl p 6, to Reduce Allergenic Activity and Preserve Immunogenicity J. Immunol., August 1, 2007; 179(3): 1730 - 1739. [Abstract] [Full Text] [PDF]

				I. Swoboda, A. Bugajska-Schretter, B. Linhart, P. Verdino, W. Keller, U. Schulmeister, W. R. Sperr, P. Valent, G. Peltre, S. Quirce, et al. A Recombinant Hypoallergenic Parvalbumin Mutant for Immunotherapy of IgE-Mediated Fish Allergy J. Immunol., May 15, 2007; 178(10): 6290 - 6296. [Abstract] [Full Text] [PDF]

				B. Linhart, S. Bigenzahn, A. Hartl, C. Lupinek, J. Thalhamer, R. Valenta, and T. Wekerle Costimulation Blockade Inhibits Allergic Sensitization but Does Not Affect Established Allergy in a Murine Model of Grass Pollen Allergy J. Immunol., March 15, 2007; 178(6): 3924 - 3931. [Abstract] [Full Text] [PDF]

				I. Rauter, M.-T. Krauth, S. Flicker, A. Gieras, K. Westritschnig, S. Vrtala, N. Balic, S. Spitzauer, J. Huss-Marp, K. Brockow, et al. Allergen cleavage by effector cell-derived proteases regulates allergic inflammation FASEB J, May 1, 2006; 20(7): 967 - 969. [Abstract] [Full Text] [PDF]

				S. L. Chan, S. T. Ong, S. Y. Ong, F. T. Chew, and Y. K. Mok Nuclear magnetic resonance structure-based epitope mapping and modulation of dust mite group 13 allergen as a hypoallergen. J. Immunol., April 15, 2006; 176(8): 4852 - 4860. [Abstract] [Full Text] [PDF]

				I. Swoboda, M. Grote, P. Verdino, W. Keller, M. B. Singh, N. De Weerd, W. R. Sperr, P. Valent, N. Balic, R. Reichelt, et al. Molecular Characterization of Polygalacturonases as Grass Pollen-Specific Marker Allergens: Expulsion from Pollen via Submicronic Respirable Particles J. Immunol., May 15, 2004; 172(10): 6490 - 6500. [Abstract] [Full Text] [PDF]

				K. Westritschnig, M. Focke, P. Verdino, W. Goessler, W. Keller, A. Twardosz, A. Mari, F. Horak, U. Wiedermann, A. Hartl, et al. Generation of an Allergy Vaccine by Disruption of the Three-Dimensional Structure of the Cross-Reactive Calcium-Binding Allergen, Phl p 7 J. Immunol., May 1, 2004; 172(9): 5684 - 5692. [Abstract] [Full Text] [PDF]

				O. Maglio, J. W. Saldanha, S. Vrtala, S. Spitzauer, R. Valenta, and A. Pastore A major IgE epitope-containing grass pollen allergen domain from Phl p 5 folds as a four-helix bundle Protein Eng. Des. Sel., August 1, 2002; 15(8): 635 - 642. [Abstract] [Full Text] [PDF]

				I. Swoboda, A. Bugajska-Schretter, P. Verdino, W. Keller, W. R. Sperr, P. Valent, R. Valenta, and S. Spitzauer Recombinant Carp Parvalbumin, the Major Cross-Reactive Fish Allergen: A Tool for Diagnosis and Therapy of Fish Allergy J. Immunol., May 1, 2002; 168(9): 4576 - 4584. [Abstract] [Full Text] [PDF]

				S. Jilek, C. Barbey, F. Spertini, and B. Corthesy Antigen-Independent Suppression of the Allergic Immune Response to Bee Venom Phospholipase A2 by DNA Vaccination in CBA/J Mice J. Immunol., March 1, 2001; 166(5): 3612 - 3621. [Abstract] [Full Text] [PDF]

				S. Vrtala, C. A. Akdis, F. Budak, M. Akdis, K. Blaser, D. Kraft, and R. Valenta T Cell Epitope-Containing Hypoallergenic Recombinant Fragments of the Major Birch Pollen Allergen, Bet v 1, Induce Blocking Antibodies J. Immunol., December 1, 2000; 165(11): 6653 - 6659. [Abstract] [Full Text] [PDF]

				S. Flicker, S. Vrtala, P. Steinberger, L. Vangelista, A. Bufe, A. Petersen, M. Ghannadan, W. R. Sperr, P. Valent, L. Norderhaug, et al. A Human Monoclonal IgE Antibody Defines a Highly Allergenic Fragment of the Major Timothy Grass Pollen Allergen, Phl p 5: Molecular, Immunological, and Structural Characterization of the Epitope-Containing Domain J. Immunol., October 1, 2000; 165(7): 3849 - 3859. [Abstract] [Full Text] [PDF]

				M. Astori, C. von Garnier, A. Kettner, N. Dufour, G. Corradin, and F. Spertini Inducing Tolerance by Intranasal Administration of Long Peptides in Naive and Primed CBA/J Mice J. Immunol., September 15, 2000; 165(6): 3497 - 3505. [Abstract] [Full Text] [PDF]

				R. J. J. van Neerven, T. Wikborg, G. Lund, B. Jacobsen, A. Brinch-Nielsen, J. Arnved, and H. Ipsen Blocking Antibodies Induced by Specific Allergy Vaccination Prevent the Activation of CD4+ T Cells by Inhibiting Serum-IgE-Facilitated Allergen Presentation J. Immunol., September 1, 1999; 163(5): 2944 - 2952. [Abstract] [Full Text] [PDF]

				T. BALL, T. FUCHS, W. R. SPERR, P. VALENT, L. VANGELISTA, D. KRAFT, and R. VALENTA B cell epitopes of the major timothy grass pollen allergen, Phl p 1, revealed by gene fragmentation as candidates for immunotherapy FASEB J, August 1, 1999; 13(11): 1277 - 1290. [Abstract] [Full Text]