| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Clinical Viro-Immunology and
Experimental Immunohematology, Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, and Laboratory of Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, and
Department of Hematology, Academic Medical Center, Amsterdam, The Netherlands
To dissect intracellular pathways involved in B cell Ag receptor (BCR)-mediated and Fas-induced human B cell death, we isolated clones of the Burkitt lymphoma cell line Ramos with different apoptosis sensitivities. Selection for sensitivity to Fas-induced apoptosis also selected for clones with enhanced BCR death sensitivity and vice versa. In contrast, clones resistant to Fas-mediated apoptosis could still undergo BCR-induced cell death. Based on the functional phenotypes of these clones, we hypothesized that both receptor-induced apoptosis pathways are initially distinct but may eventually converge. Indeed, ligation of both Fas and BCR resulted in cleavage of the IL-1ß-converting enzyme/Ced-3-like protease caspase 3 and its substrates Ac-Asp-Glu-Val-Asp-aldehyde and poly(ADP-ribose) polymerase. Markedly, qualitative differences in the caspase 3 cleavage pattern induced by Fas or BCR ligation were observed; whereas Fas ligation generated caspase 3 cleavage products of 19/20 and 17 kDa, only the latter cleavage product was found upon BCR cross-linking. The caspase inhibitor Val-Ala-Asp-fluoromethylketone blocked both Fas- and BCR-mediated apoptosis, but differentially affected caspase 3 cleavage induced by either stimulus. Finally, overexpression of a Fas-associated death domain (FADD) dominant-negative mutant protein was found to inhibit Fas-induced apoptosis but not BCR-induced apoptosis. Together our findings imply that Fas and BCR couple, via FADD-dependent and FADD-independent mechanisms, respectively, to distinct proteases upstream of caspase 3.
This article has been cited by other articles:
|
|
 |
|
  A. Ruiz-Vela, M. Aggarwal, P. de la Cueva, C. Treda, B. Herreros, D. Martin-Perez, O. Dominguez, and M. A. Piris Lentiviral (HIV)-based RNA interference screen in human B-cell receptor regulatory networks reveals MCL1-induced oncogenic pathways Blood, February 1, 2008; 111(3): 1665 - 1676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Smit, D. Y.H. Hallaert, R. Spijker, B. de Goeij, A. Jaspers, A. P. Kater, M. H.J. van Oers, C. J.M. van Noesel, and E. Eldering Differential Noxa/Mcl-1 balance in peripheral versus lymph node chronic lymphocytic leukemia cells correlates with survival capacity Blood, February 15, 2007; 109(4): 1660 - 1668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Poggianella, M. Bestagno, and O. R. Burrone The Extracellular Membrane-Proximal Domain of Human Membrane IgE Controls Apoptotic Signaling of the B Cell Receptor in the Mature B Cell Line A20 J. Immunol., September 15, 2006; 177(6): 3597 - 3605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Xing, H. Igarashi, X. Wang, and N. Sakaguchi Protein phosphatase subunit G5PR is needed for inhibition of B cell receptor-induced apoptosis J. Exp. Med., September 6, 2005; 202(5): 707 - 719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Enders, P. Bouillet, H. Puthalakath, Y. Xu, D. M. Tarlinton, and A. Strasser Loss of the Pro-Apoptotic BH3-only Bcl-2 Family Member Bim Inhibits BCR Stimulation-induced Apoptosis and Deletion of Autoreactive B Cells J. Exp. Med., October 6, 2003; 198(7): 1119 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Goodyear and G. J. Silverman Death by a B Cell Superantigen: In Vivo VH-targeted Apoptotic Supraclonal B Cell Deletion by a Staphylococcal Toxin J. Exp. Med., May 5, 2003; 197(9): 1125 - 1139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Muscarella and S. E. Bloom Cross-linking of Surface IgM in the Burkitt's Lymphoma Cell Line ST486 Provides Protection against Arsenite- and Stress-induced Apoptosis That Is Mediated by ERK and Phosphoinositide 3-Kinase Signaling Pathways J. Biol. Chem., January 31, 2003; 278(6): 4358 - 4367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hase, Y. Kanno, H. Kojima, C. Morimoto, K. Okumura, and T. Kobata CD27 and CD40 Inhibit p53-independent Mitochondrial Pathways in Apoptosis of B Cells Induced by B Cell Receptor Ligation J. Biol. Chem., November 27, 2002; 277(49): 46950 - 46958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J. M. Mackus, S. M. A. Lens, R. H. Medema, M. J. Kwakkenbos, L. M. Evers, M. H. J. v. Oers, R. A. W. v. Lier, and E. Eldering Prevention of B cell antigen receptor-induced apoptosis by ligation of CD40 occurs downstream of cell cycle regulation Int. Immunol., September 1, 2002; 14(9): 973 - 982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chu, D. Deforce, I. M. Pedersen, Y. Kim, S. Kitada, J. C. Reed, and T. J. Kipps Latent sensitivity to Fas-mediated apoptosis after CD40 ligation may explain activity of CD154 gene therapy in chronic lymphocytic leukemia PNAS, March 19, 2002; 99(6): 3854 - 3859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yoshikawa, Y. Nakajima, and K. Tasaka IFN-{gamma} Induces the Apoptosis of WEHI 279 and Normal Pre-B Cell Lines by Expressing Direct Inhibitor of Apoptosis Protein Binding Protein with Low pI J. Immunol., September 1, 2001; 167(5): 2487 - 2495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-K. Hofmann, S. de Vos, K. Tsukasaki, W. Wachsman, G. S. Pinkus, J. W. Said, and H. P. Koeffler Altered apoptosis pathways in mantle cell lymphoma detected by oligonucleotide microarray Blood, August 1, 2001; 98(3): 787 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Besnault, N. Schrantz, M. T. Auffredou, G. Leca, M. F. Bourgeade, and A. Vazquez B Cell Receptor Cross-Linking Triggers a Caspase-8- Dependent Apoptotic Pathway That Is Independent of the Death Effector Domain of Fas-Associated Death Domain Protein J. Immunol., July 15, 2001; 167(2): 733 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Tian, C.-H. G. Chou, and A. L. DeFranco Apoptosis induced by the antigen receptor and Fas in a variant of the immature B cell line WEHI-231 and in splenic immature B cells Int. Immunol., April 1, 2001; 13(4): 581 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Takada, H. Toyota, J. Suzuki, and J. Mizuguchi Prevention of Anti-IgM-Induced Apoptosis Accompanying G1 Arrest in B Lymphoma Cells Overexpressing Dominant-Negative Mutant Form of c-Jun N-Terminal Kinase 1 J. Immunol., February 1, 2001; 166(3): 1641 - 1649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Baars, L. M. Ribeiro do Couto, J. H. W. Leusen, B. Hooibrink, T. W. Kuijpers, S. M. A. Lens, and R. A. W. van Lier Cytolytic Mechanisms and Expression of Activation-Regulating Receptors on Effector-Type CD8+CD45RA+CD27- Human T Cells J. Immunol., August 15, 2000; 165(4): 1910 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yoshida, T. Higuchi, H. Hagiyama, A. Strasser, K. Nishioka, and T. Tsubata Rapid B cell apoptosis induced by antigen receptor ligation does not require Fas (CD95/APO-1), the adaptor protein FADD/MORT1 or CrmA-sensitive caspases but is defective in both MRL-+/+ and MRL-lpr/lpr mice Int. Immunol., April 1, 2000; 12(4): 517 - 526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Berard, P. Mondiere, M. Casamayor-Palleja, A. Hennino, C. Bella, and T. Defrance Mitochondria Connects the Antigen Receptor to Effector Caspases During B Cell Receptor-Induced Apoptosis in Normal Human B Cells J. Immunol., November 1, 1999; 163(9): 4655 - 4662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Chen, H.-G. Wang, S. M. Srinivasula, E. S. Alnemri, and N. R. Cooper B Cell Apoptosis Triggered by Antigen Receptor Ligation Proceeds Via a Novel Caspase-Dependent Pathway J. Immunol., September 1, 1999; 163(5): 2483 - 2491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Doi, N. Motoyama, A. Tokunaga, and T. Watanabe Death signals from the B cell antigen receptor target mitochondria, activating necrotic and apoptotic death cascades in a murine B cell line, WEHI-231 Int. Immunol., June 1, 1999; 11(6): 933 - 941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Karp, K. Shimooku, and P. E. Lipsky Expression of gamma -Glutamyl Transpeptidase Protects Ramos B Cells from Oxidation-induced Cell Death J. Biol. Chem., February 2, 2001; 276(6): 3798 - 3804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Marches, E. S. Vitetta, and J. W. Uhr A role for intracellular pH in membrane IgM-mediated cell death of human B lymphomas PNAS, March 13, 2001; 98(6): 3434 - 3439. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
