| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of
*
Microbiology and
Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78284
CMV retinitis, the most common ophthalmic infection of AIDS patients, causes blindness if left untreated. To study the role of NK cells in the modulation of CMV ocular infection, 9.0 x 102 plaque-forming units of the Smith strain of murine CMV (MCMV) was injected into the supraciliary space of the left eyes of BALB/c mice. Lysis of NK-sensitive target cells (YAC-1) by effectors from the draining lymph nodes peaked at day 5 postinfection, while the splenic cytolytic response was biphasic, with peaks at days 2 and 7 postinfection. Flow cytometry showed that NK cells (DX-5+) increased in spleens and eyes 5 days after supraciliary infection with MCMV compared with uninfected or mock-infected controls. Eight days after supraciliary injection with 9.0 x 102 plaque-forming units of MCMV, 7 of 10 NK-depleted mice developed retinitis compared with only 2 of 10 non-NK-depleted control mice. Poly(I-C) activation of NK cells in T cell-depleted animals protected mice from MCMV retinitis; only 2 of 10 mice in the poly(I-C)-treated group developed retinitis compared with 8 of 10 T cell-depleted, non-poly(I-C)-treated control mice. These results show the importance of NK cells in preventing MCMV retinitis and suggest that NK cells may also be involved in modulation of cytomegalovirus retinitis in human patients.
This article has been cited by other articles:
|
|
 |
|
  M. Zhang, J. Zhou, B. Marshall, H. Xin, and S. S. Atherton Lack of iNOS Facilitates MCMV Spread in the Retina Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 285 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Reuter, J. H. Wilson, K. E Idoko, and A. N. van den Pol CD4+ T-Cell Reconstitution Reduces Cytomegalovirus in the Immunocompromised Brain J. Virol., August 1, 2005; 79(15): 9527 - 9539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Dix, E. R. Podack, and S. W. Cousins Loss of the Perforin Cytotoxic Pathway Predisposes Mice to Experimental Cytomegalovirus Retinitis J. Virol., March 15, 2003; 77(6): 3402 - 3408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Johnson, S. Hong, L. Van Kaer, Y. Koezuka, and B. S. Graham NK T Cells Contribute to Expansion of CD8+ T Cells and Amplification of Antiviral Immune Responses to Respiratory Syncytial Virus J. Virol., March 27, 2002; 76(9): 4294 - 4303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Bigger, M. Tanigawa, M. Zhang, and S. S. Atherton Murine Cytomegalovirus Infection Causes Apoptosis of Uninfected Retinal Cells Invest. Ophthalmol. Vis. Sci., July 1, 2000; 41(8): 2248 - 2254. [Abstract] [Full Text]
|
|
|
|
|
|
M. Tanigawa, J. E. Bigger, M. Y. Kanter, and S. S. Atherton Natural Killer Cells Prevent Direct Anterior-to-Posterior Spread of Herpes Simplex Virus Type 1 in the Eye Invest. Ophthalmol. Vis. Sci., January 1, 2000; 41(1): 132 - 137. [Abstract] [Full Text]
|
|
|
|
|
|
J. E. Bigger, M. Tanigawa, C. A. Thomas III, and S. S. Atherton Protection against Murine Cytomegalovirus Retinitis by Adoptive Transfer of Virus-Specific CD8+ T Cells Invest. Ophthalmol. Vis. Sci., October 1, 1999; 40(11): 2608 - 2613. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
