The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, K.
Right arrow Articles by Durum, S. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, K.
Right arrow Articles by Durum, S. K.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Medline Plus Health Information
*Stem Cells
The Journal of Immunology, 1998, 160: 5735-5741.
Copyright © 1998 by The American Association of Immunologists

The Trophic Action of IL-7 on Pro-T Cells: Inhibition of Apoptosis of Pro-T1, -T2, and -T3 Cells Correlates with Bcl-2 and Bax Levels and Is Independent of Fas and p53 Pathways1

Kyungjae Kim*, Chong-kil Lee*, Thomas J. Sayers{dagger}, Kathrin Muegge{dagger} and Scott K. Durum*

* Laboratory of Molecular Immunoregulation and {dagger} Science Applications International Corporation, Division of Basic Sciences, National Cancer Institute, Frederick, MD 21702

Signals from the IL-7R are essential for normal thymocyte development. We isolated thymocytes from early developmental stages and observed that suspensions of pro-T1, -T2, and -T3 cells rapidly died in culture. Addition of IL-7 promoted their survival, but did not induce cell division. Pro-T4 cells did not undergo rapid cell death, and their survival was therefore independent of IL-7. Death in the absence of IL-7 showed the hallmarks of apoptosis, including DNA fragmentation and annexin V binding; however, caspase inhibitors blocked DNA fragmentation, but did not block cell death. The trophic effect of IL-7 was partially inhibited by blocking protein synthesis. The p53 pathway was not involved in this death pathway, since pro-T cells from p53-/- mice also underwent cell death in the absence of IL-7. The Fas/Fas ligand pathway was not involved in cell death, since Fas-deficient pro-T cells died normally in the absence of IL-7, anti-Fas Abs did not protect cells from death in the absence of IL-7, and Fas expression was undetectable on cells at these stages. The IL-7 trophic affect correlated with increased intracellular levels of Bcl-2 and decreased levels of Bax, whereas no Bcl-XL, Bcl-w, or Bad was detectable. Thus, maintaining a favorable Bcl-2/Bax ratio may account for the trophic action of IL-7.




This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
E. Di Carlo, T. D'Antuono, P. Pompa, R. Giuliani, S. Rosini, L. Stuppia, P. Musiani, and C. Sorrentino
The Lack of Epithelial Interleukin-7 and BAFF/BLyS Gene Expression in Prostate Cancer as a Possible Mechanism of Tumor Escape from Immunosurveillance
Clin. Cancer Res., May 1, 2009; 15(9): 2979 - 2987.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Rochman and W. J. Leonard
The Role of Thymic Stromal Lymphopoietin in CD8+ T Cell Homeostasis
J. Immunol., December 1, 2008; 181(11): 7699 - 7705.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. Mazzucchelli, J. A. Hixon, R. Spolski, X. Chen, W. Q. Li, V. L. Hall, J. Willette-Brown, A. A. Hurwitz, W. J. Leonard, and S. K. Durum
Development of regulatory T cells requires IL-7R{alpha} stimulation by IL-7 or TSLP
Blood, October 15, 2008; 112(8): 3283 - 3292.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
S. M. Churchman and F. Ponchel
Interleukin-7 in rheumatoid arthritis
Rheumatology, June 1, 2008; 47(6): 753 - 759.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Yang, J. An, and N.-p. Weng
Telomerase Is Involved in IL-7-Mediated Differential Survival of Naive and Memory CD4+ T Cells
J. Immunol., March 15, 2008; 180(6): 3775 - 3781.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. P. Andrews, C. D. Pack, V. Vezys, G. N. Barber, and A. E. Lukacher
Early Virus-Associated Bystander Events Affect the Fitness of the CD8 T Cell Response to Persistent Virus Infection
J. Immunol., June 1, 2007; 178(11): 7267 - 7275.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Min, A. Panoskaltsis-Mortari, M. Kuro-o, G. A. Hollander, B. R. Blazar, and K. I. Weinberg
Sustained thymopoiesis and improvement in functional immunity induced by exogenous KGF administration in murine models of aging
Blood, March 15, 2007; 109(6): 2529 - 2537.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Vassena, M. Proschan, A. S. Fauci, and P. Lusso
Interleukin 7 reduces the levels of spontaneous apoptosis in CD4+ and CD8+ T cells from HIV-1-infected individuals
PNAS, February 13, 2007; 104(7): 2355 - 2360.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Q. Jiang, J. Huang, W. Q. Li, T. Cavinato, J. R. Keller, and S. K. Durum
Role of the Intracellular Domain of IL-7 Receptor in T Cell Development
J. Immunol., January 1, 2007; 178(1): 228 - 234.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
W. Q. Li, Q. Jiang, E. Aleem, P. Kaldis, A. R. Khaled, and S. K. Durum
IL-7 promotes T cell proliferation through destabilization of p27Kip1
J. Exp. Med., March 20, 2006; 203(3): 573 - 582.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K.-i. Yamanaka, R. Clark, B. Rich, R. Dowgiert, K. Hirahara, D. Hurwitz, M. Shibata, N. Mirchandani, D. A. Jones, D. S. Goddard, et al.
Skin-derived interleukin-7 contributes to the proliferation of lymphocytes in cutaneous T-cell lymphoma
Blood, March 15, 2006; 107(6): 2440 - 2445.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
Q. Yu, J.-H. Park, L. L. Doan, B. Erman, L. Feigenbaum, and A. Singer
Cytokine signal transduction is suppressed in preselection double-positive thymocytes and restored by positive selection
J. Exp. Med., January 23, 2006; 203(1): 165 - 175.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. S. P. Heng, G. L. Goldberg, D. H. D. Gray, J. S. Sutherland, A. P. Chidgey, and R. L. Boyd
Effects of Castration on Thymocyte Development in Two Different Models of Thymic Involution
J. Immunol., September 1, 2005; 175(5): 2982 - 2993.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Vasir, D. Avigan, Z. Wu, K. Crawford, S. Turnquist, J. Ren, and D. Kufe
Dendritic Cells Induce MUC1 Expression and Polarization on Human T Cells by an IL-7-Dependent Mechanism
J. Immunol., February 15, 2005; 174(4): 2376 - 2386.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
I. Munitic, J. A. Williams, Y. Yang, B. Dong, P. J. Lucas, N. El Kassar, R. E. Gress, and J. D. Ashwell
Dynamic regulation of IL-7 receptor expression is required for normal thymopoiesis
Blood, December 15, 2004; 104(13): 4165 - 4172.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
T. J. Hagenbeek, M. Naspetti, F. Malergue, F. Garcon, J. A. Nunes, K. B.J.M. Cleutjens, J. Trapman, P. Krimpenfort, and H. Spits
The Loss of PTEN Allows TCR {alpha}{beta} Lineage Thymocytes to Bypass IL-7 and Pre-TCR-mediated Signaling
J. Exp. Med., October 4, 2004; 200(7): 883 - 894.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Esashi, H. Ito, K. Ishihara, T. Hirano, S. Koyasu, and A. Miyajima
Development of CD4+ Macrophages from Intrathymic T Cell Progenitors Is Induced by Thymic Epithelial Cells
J. Immunol., October 1, 2004; 173(7): 4360 - 4367.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
Q. Yu, B. Erman, J.-H. Park, L. Feigenbaum, and A. Singer
IL-7 Receptor Signals Inhibit Expression of Transcription Factors TCF-1, LEF-1, and ROR{gamma}t: Impact on Thymocyte Development
J. Exp. Med., September 20, 2004; 200(6): 797 - 803.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Q. Li, Q. Jiang, A. R. Khaled, J. R. Keller, and S. K. Durum
Interleukin-7 Inactivates the Pro-apoptotic Protein Bad Promoting T Cell Survival
J. Biol. Chem., July 9, 2004; 279(28): 29160 - 29166.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. J. Van De Wiele, J. H. Marino, B. W. Murray, S. S. Vo, M. E. Whetsell, and T. K. Teague
Thymocytes between the {beta}-Selection and Positive Selection Checkpoints Are Nonresponsive to IL-7 as Assessed by STAT-5 Phosphorylation
J. Immunol., April 1, 2004; 172(7): 4235 - 4244.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. T. Barata, V. A. Boussiotis, J. A. Yunes, A. A. Ferrando, L. A. Moreau, J. P. Veiga, S. E. Sallan, A. T. Look, L. M. Nadler, and A. A. Cardoso
IL-7-dependent human leukemia T-cell line as a valuable tool for drug discovery in T-ALL
Blood, March 1, 2004; 103(5): 1891 - 1900.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Muthukumar, A. Wozniakowski, M.-C. Gauduin, M. Paiardini, H. M. McClure, R. P. Johnson, G. Silvestri, and D. L. Sodora
Elevated interleukin-7 levels not sufficient to maintain T-cell homeostasis during simian immunodeficiency virus-induced disease progression
Blood, February 1, 2004; 103(3): 973 - 979.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. C. Jung, W. S. Park, H. J. Kim, E. Y. Choi, M.-C. Kook, H.-W. Lee, and Y. Bae
TCR-Independent and Caspase-Independent Apoptosis of Murine Thymocytes by CD24 Cross-Linking
J. Immunol., January 15, 2004; 172(2): 795 - 802.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Bhandoola, A. Sambandam, D. Allman, A. Meraz, and B. Schwarz
Early T Lineage Progenitors: New Insights, but Old Questions Remain
J. Immunol., December 1, 2003; 171(11): 5653 - 5658.
[Full Text] [PDF]

Home page
 BloodHome page
V. Mateo, E. J. Brown, G. Biron, M. Rubio, A. Fischer, F. L. Deist, and M. Sarfati
Mechanisms of CD47-induced caspase-independent cell death in normal and leukemic cells: link between phosphatidylserine exposure and cytoskeleton organization
Blood, September 26, 2002; 100(8): 2882 - 2890.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. J. Fry and C. L. Mackall
Interleukin-7: from bench to clinic
Blood, May 13, 2002; 99(11): 3892 - 3904.
[Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. R. Khaled, A. N. Moor, A. Li, K. Kim, D. K. Ferris, K. Muegge, R. J. Fisher, L. Fliegel, and S. K. Durum
Trophic Factor Withdrawal: p38 Mitogen-Activated Protein Kinase Activates NHE1, Which Induces Intracellular Alkalinization
Mol. Cell. Biol., November 15, 2001; 21(22): 7545 - 7557.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
E. Guillemard, M.-T. Nugeyre, L. Chene, N. Schmitt, C. Jacquemot, F. Barre-Sinoussi, and N. Israel
Interleukin-7 and infection itself by human immunodeficiency virus 1 favor virus persistence in mature CD4+CD8{-}CD3+ thymocytes through sustained induction of Bcl-2
Blood, October 1, 2001; 98(7): 2166 - 2174.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. T. Barata, A. A. Cardoso, L. M. Nadler, and V. A. Boussiotis
Interleukin-7 promotes survival and cell cycle progression of T-cell acute lymphoblastic leukemia cells by down-regulating the cyclin-dependent kinase inhibitor p27kip1
Blood, September 1, 2001; 98(5): 1524 - 1531.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C.-K. Lee, J. K. Kim, Y. Kim, M.-K. Lee, K. Kim, J.-K. Kang, R. Hofmeister, S. K. Durum, and S. S. Han
Generation of Macrophages from Early T Progenitors In Vitro
J. Immunol., May 15, 2001; 166(10): 5964 - 5969.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Trop, P. De Sepulveda, J. C. Zuniga-Pflucker, and R. Rottapel
Overexpression of suppressor of cytokine signaling-1 impairs pre-T-cell receptor-induced proliferation but not differentiation of immature thymocytes
Blood, April 15, 2001; 97(8): 2269 - 2277.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. A. Welniak, A. R. Khaled, M. R. Anver, K. L. Komschlies, R. H. Wiltrout, S. Durum, F. R. Ruscetti, B. R. Blazar, and W. J. Murphy
Gastrointestinal Cells of IL-7 Receptor Null Mice Exhibit Increased Sensitivity to Irradiation
J. Immunol., March 1, 2001; 166(5): 2923 - 2928.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Andrew and R. Aspinall
IL-7 and Not Stem Cell Factor Reverses Both the Increase in Apoptosis and the Decline in Thymopoiesis Seen in Aged Mice
J. Immunol., February 1, 2001; 166(3): 1524 - 1530.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. G. Baseta and O. Stutman
TNF Regulates Thymocyte Production by Apoptosis and Proliferation of the Triple Negative (CD3-CD4-CD8-) Subset
J. Immunol., November 15, 2000; 165(10): 5621 - 5630.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. M. Baird, J. A. Lucas, and L. J. Berg
A Profound Deficiency in Thymic Progenitor Cells in Mice Lacking Jak3
J. Immunol., October 1, 2000; 165(7): 3680 - 3688.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Ghatan, S. Larner, Y. Kinoshita, M. Hetman, L. Patel, Z. Xia, R. J. Youle, and R. S. Morrison
p38 Map Kinase Mediates Bax Translocation in Nitric Oxide-Induced Apoptosis in Neurons
J. Cell Biol., July 24, 2000; 150(2): 335 - 348.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
P. S. Costello, S. C. Cleverley, R. Galandrini, S. W. Henning, and D. A. Cantrell
The Gtpase Rho Controls a P53-Dependent Survival Checkpoint during Thymopoiesis
J. Exp. Med., July 3, 2000; 192(1): 77 - 86.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. Karawajew, V. Ruppert, C. Wuchter, A. Kosser, M. Schrappe, B. Dorken, and W.-D. Ludwig
Inhibition of in vitro spontaneous apoptosis by IL-7 correlates with Bcl-2 up-regulation, cortical/mature immunophenotype, and better early cytoreduction of childhood T-cell acute lymphoblastic leukemia
Blood, July 1, 2000; 96(1): 297 - 306.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Zhang and T. J. Rogers
{kappa}-Opioid Regulation of Thymocyte IL-7 Receptor and C-C Chemokine Receptor 2 Expression
J. Immunol., May 15, 2000; 164(10): 5088 - 5093.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
M. S. Schlissel, S. D. Durum, and K. Muegge
The Interleukin 7 Receptor Is Required for T Cell Receptor {gamma} Locus Accessibility to the V(D)j Recombinase
J. Exp. Med., March 20, 2000; 191(6): 1045 - 1050.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Benbernou, K. Muegge, and S. K. Durum
Interleukin (IL)-7 Induces Rapid Activation of Pyk2, Which Is Bound to Janus Kinase 1 and IL-7Ralpha
J. Biol. Chem., March 15, 2000; 275(10): 7060 - 7065.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. R. Khaled, K. Kim, R. Hofmeister, K. Muegge, and S. K. Durum
From the Cover: Withdrawal of IL-7 induces Bax translocation from cytosol to mitochondria through a rise in intracellular pH
PNAS, December 7, 1999; 96(25): 14476 - 14481.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. D. Johnson, Y. Kinoshita, H. Xiang, S. Ghatan, and R. S. Morrison
Contribution of p53-Dependent Caspase Activation to Neuronal Cell Death Declines with Neuronal Maturation
J. Neurosci., April 15, 1999; 19(8): 2996 - 3006.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. K. Durum, S. Candeias, H. Nakajima, W. J. Leonard, A. M. Baird, L. J. Berg, and K. Muegge
Interleukin 7 Receptor Control of T Cell Receptor {gamma} Gene Rearrangement: Role of Receptor-associated Chains and Locus Accessibility
J. Exp. Med., December 21, 1998; 188(12): 2233 - 2241.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1998 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1998 by The American Association of Immunologists, Inc. All rights reserved.