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Quercetin Sensitizes RBL-2H3 Cells to Polybasic Mast Cell Secretagogues Through Increased Expression of G_i GTP-Binding Proteins Linked to a Phospholipase C Signaling Pathway

Laboratory of Molecular Immunology, National Institute of Heart, Lung, and Blood Diseases, National Institutes of Health, Bethesda, MD 20892

Polybasic secretagogues such as mastoparan, compound 48/80, substance P, and somatostatin stimulate secretion in rat peritoneal mast cells through direct activation of the heterotrimeric G protein, G_i-3. Cultured RBL-2H3 mast cells do not normally respond to these secretagogues, but, as reported here, they do so after prolonged exposure to the kinase inhibitor, quercetin. This inhibitor, which causes phenotypic changes in RBL-2H3 cells, induces a substantial increase (more than sevenfold) in the expression of subunits of the pertussis toxin-sensitive G proteins, G_i-2 and G_i-3. Compound 48/80-induced secretion is associated with transient hydrolysis of phosphoinositides and a transient increase in cytosolic calcium ions. These responses are inhibited by pertussis toxin, and in addition, secretion is blocked by calcium chelation and the protein kinase C inhibitor, Ro31-7549. These results delineate a pathway for compound 48/80-induced secretion in mast cells via G_i protein(s), phospholipase C, calcium, and protein kinase C. The results also imply that phospholipase C, most likely phospholipase Cß3, can be transiently activated in RBL-2H3 cells by subunits of G_i proteins to induce cellular responses.

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