HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gibson, F. C. Articles by Tzianabos, A. O. Search for Related Content PubMed PubMed Citation Articles by Gibson, F. C., III Articles by Tzianabos, A. O.

Cellular Mechanism of Intraabdominal Abscess Formation by Bacteroides fragilis¹

Frank C. Gibson, III^2,*, Andrew B. Onderdonk, Dennis L. Kasper^* and Arthur O. Tzianabos^*

Channing Laboratory, Departments of ^* Medicine and Pathology, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115

We investigated the cellular mechanism by which Bacteroides fragilis promotes the development of intraabdominal abscesses in experimental models of sepsis. B. fragilis, as well as purified capsular polysaccharide complex (CPC) from this organism, adhered to primary murine mesothelial cells (MMCs) in vitro. The binding of CPC to murine peritoneal macrophage stimulated TNF- production, which when transferred to monolayers of MMCs elicited significant ICAM-1 expression by these cells. This response resulted in enhanced polymorphonuclear leukocyte attachment to MMCs that could be inhibited by Abs specific for TNF- or ICAM-1. Mice treated with TNF-- or ICAM-1-specific Abs failed to develop intraabdominal abscesses following challenge with purified CPC. These results illustrated the role of the CPC in promoting adhesion of B. fragilis to the peritoneal wall and coordinating the cellular events leading to the development of abscesses associated with experimental intraabdominal sepsis.

This article has been cited by other articles:

				G. d'Empaire, M. T. Baer, and F. C. Gibson III The K1 Serotype Capsular Polysaccharide of Porphyromonas gingivalis Elicits Chemokine Production from Murine Macrophages That Facilitates Cell Migration Infect. Immun., November 1, 2006; 74(11): 6236 - 6243. [Abstract] [Full Text] [PDF]

				K. P. Robertson, C. J. Smith, A. M. Gough, and E. R. Rocha Characterization of Bacteroides fragilis Hemolysins and Regulation and Synergistic Interactions of HlyA and HlyB Infect. Immun., April 1, 2006; 74(4): 2304 - 2316. [Abstract] [Full Text] [PDF]

				I. K. Mullarky, F. M. Szaba, K. N. Berggren, L. W. Kummer, L. B. Wilhelm, M. A. Parent, L. L. Johnson, and S. T. Smiley Tumor Necrosis Factor Alpha and Gamma Interferon, but Not Hemorrhage or Pathogen Burden, Dictate Levels of Protective Fibrin Deposition during Infection Infect. Immun., February 1, 2006; 74(2): 1181 - 1188. [Abstract] [Full Text] [PDF]

				R. K. Gupta, K. M. Hasan, A. M. Mishra, D. Jha, M. Husain, K. N. Prasad, and P. A. Narayana High Fractional Anisotropy in Brain Abscesses versus Other Cystic Intracranial Lesions AJNR Am. J. Neuroradiol., May 1, 2005; 26(5): 1107 - 1114. [Abstract] [Full Text] [PDF]

				F. Stingele, B. Corthesy, N. Kusy, S. A. Porcelli, D. L. Kasper, and A. O. Tzianabos Zwitterionic Polysaccharides Stimulate T Cells with No Preferential V{beta} Usage and Promote Anergy, Resulting in Protection against Experimental Abscess Formation J. Immunol., February 1, 2004; 172(3): 1483 - 1490. [Abstract] [Full Text] [PDF]

				S. M. L. Montenegro, F. G. C. Abath, A. L. C. Domingues, W. G. Melo, C. N. L. Morais, E. M. Coutinho, S. Mahanty, and T. A. Wynn Enhanced Interleukin-12 and CD40 Ligand Activities but Reduced Staphylococcus aureus Cowan 1-Induced Responses Suggest a Generalized and Progressively Impaired Type 1 Cytokine Pattern for Human Schistosomiasis Infect. Immun., November 1, 2002; 70(11): 5903 - 5912. [Abstract] [Full Text] [PDF]

				L. R. Schopf, K. F. Hoffmann, A. W. Cheever, J. F. Urban Jr., and T. A. Wynn IL-10 Is Critical for Host Resistance and Survival During Gastrointestinal Helminth Infection J. Immunol., March 1, 2002; 168(5): 2383 - 2392. [Abstract] [Full Text] [PDF]

				B. Echtenacher, K. Weigl, N. Lehn, and D. N. Mannel Tumor Necrosis Factor-Dependent Adhesions as a Major Protective Mechanism Early in Septic Peritonitis in Mice Infect. Immun., June 1, 2001; 69(6): 3550 - 3555. [Abstract] [Full Text] [PDF]

				M. M. P. J. Reijnen, H. van Goor, P. Falk, M. Hedgren, and L. Holmdahl Sodium Hyaluronate Increases the Fibrinolytic Response of Human Peritoneal Mesothelial Cells Exposed to Tumor Necrosis Factor {alpha} Arch Surg, March 1, 2001; 136(3): 291 - 296. [Abstract] [Full Text] [PDF]

				A. O. Tzianabos, A. Chandraker, W. Kalka-Moll, F. Stingele, V. M. Dong, R. W. Finberg, R. Peach, and M. H. Sayegh Bacterial Pathogens Induce Abscess Formation by CD4+ T-Cell Activation via the CD28-B7-2 Costimulatory Pathway Infect. Immun., December 1, 2000; 68(12): 6650 - 6655. [Abstract] [Full Text] [PDF]

				A. O. Tzianabos Polysaccharide Immunomodulators as Therapeutic Agents: Structural Aspects and Biologic Function Clin. Microbiol. Rev., October 1, 2000; 13(4): 523 - 533. [Abstract] [Full Text] [PDF]

				L. E. Comstock, M. J. Coyne, A. O. Tzianabos, A. Pantosti, A. B. Onderdonk, and D. L. Kasper Analysis of a Capsular Polysaccharide Biosynthesis Locus of Bacteroides fragilis Infect. Immun., July 1, 1999; 67(7): 3525 - 3532. [Abstract] [Full Text] [PDF]