HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kohda, K. Articles by Kasahara, M. Search for Related Content PubMed PubMed Citation Articles by Kohda, K. Articles by Kasahara, M.

Characterization of the Mouse PA28 Activator Complex Gene Family: Complete Organizations of the Three Member Genes and a Physical Map of the 150-kb Region Containing the - and ß-Subunit Genes^{1 ,2}

Keiko Kohda^*, Teruo Ishibashi^*, Naoki Shimbara^,, Keiji Tanaka^,§, Yoichi Matsuda^¶ and Masanori Kasahara^3,*,

^* Department of Biochemistry, Hokkaido University School of Medicine, Sapporo 060, Japan; Biomedical R&D Department, Sumitomo Electric Industries, Yokohama 244, Japan; CREST (Core Research for Evolutional Science and Technology), Japan Science and Technology Corporation, Japan; ^§ Tokyo Metropolitan Institute of Medical Science, Tokyo 113, Japan; and ^¶ Laboratory of Animal Genetics, Nagoya University School of Agricultural Sciences, Nagoya 464-01, Japan

The proteasome is a multisubunit protease responsible for the generation of peptides loaded onto MHC class I molecules. Recent evidence indicates that binding of an IFN--inducible PA28 activator complex to the 20S proteasome enhances the generation of class I binding peptides. The - and ß-subunits, which constitute the PA28 activator complex in the form of an (ß)₃ heterohexamer, show significant amino acid sequence similarity to a protein, designated Ki or the -subunit, that is capable of binding to the 20S proteasome. In this study, we describe the complete nucleotide sequences of the mouse genes, Psme1, Psme2, and Psme3, coding for the -, ß-, and -subunits, respectively. The overall exon-intron organizations of the three Psme genes are virtually identical, thus providing evidence that they are descended from a single ancestral gene. The promoter regions of the Psme1 and Psme2 genes contain sequence motifs that qualify as IFN-stimulated response elements, consistent with the observation that their expression is induced strongly by IFN-. The Psme1 and Psme2 genes are located 6 kb apart with their 3'-ends pointing toward each other on bands C2 to D1 of mouse chromosome 14, supporting the idea that they emerged by tandem duplication.

This article has been cited by other articles:

				G. M. Doody, S. Stephenson, C. McManamy, and R. M. Tooze PRDM1/BLIMP-1 Modulates IFN-{gamma}-Dependent Control of the MHC Class I Antigen-Processing and Peptide-Loading Pathway J. Immunol., December 1, 2007; 179(11): 7614 - 7623. [Abstract] [Full Text] [PDF]

				B. Khor, A. L. Bredemeyer, C.-Y. Huang, I. R. Turnbull, R. Evans, L. B. Maggi Jr., J. M. White, L. M. Walker, K. Carnes, R. A. Hess, et al. Proteasome Activator PA200 Is Required for Normal Spermatogenesis Mol. Cell. Biol., April 15, 2006; 26(8): 2999 - 3007. [Abstract] [Full Text] [PDF]

				F. Ossendorp, N. Fu, M. Camps, F. Granucci, S. J. P. Gobin, P. J. van den Elsen, D. Schuurhuis, G. J. Adema, G. B. Lipford, T. Chiba, et al. Differential Expression Regulation of the {alpha} and {beta} Subunits of the PA28 Proteasome Activator in Mature Dendritic Cells J. Immunol., June 15, 2005; 174(12): 7815 - 7822. [Abstract] [Full Text] [PDF]

				K. Moriishi, T. Okabayashi, K. Nakai, K. Moriya, K. Koike, S. Murata, T. Chiba, K. Tanaka, R. Suzuki, T. Suzuki, et al. Proteasome Activator PA28{gamma}-Dependent Nuclear Retention and Degradation of Hepatitis C Virus Core Protein J. Virol., October 1, 2003; 77(19): 10237 - 10249. [Abstract] [Full Text] [PDF]

				A. C. O. Vertegaal, H. B. Kuiperij, A. Houweling, M. Verlaan, A. J. van der Eb, and A. Zantema Differential Expression of Tapasin and Immunoproteasome Subunits in Adenovirus Type 5- Versus Type 12-transformed Cells J. Biol. Chem., January 3, 2003; 278(1): 139 - 146. [Abstract] [Full Text] [PDF]

				S. Murata, H. Kawahara, S. Tohma, K. Yamamoto, M. Kasahara, Y.-i. Nabeshima, K. Tanaka, and T. Chiba Growth Retardation in Mice Lacking the Proteasome Activator PA28gamma J. Biol. Chem., December 31, 1999; 274(53): 38211 - 38215. [Abstract] [Full Text] [PDF]

				P. Masson, O. Andersson, U.-M. Petersen, and P. Young Identification and Characterization of a Drosophila Nuclear Proteasome Regulator. A HOMOLOG OF HUMAN 11 S REGgamma (PA28gamma ) J. Biol. Chem., January 5, 2001; 276(2): 1383 - 1390. [Abstract] [Full Text] [PDF]