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Chains in Individual Naive and Memory B Cells1
Department of Internal Medicine and Harold C. Simmons Arthritis Research Center, University of Texas Southwestern Medical Center at Dallas, Dallas TX 75235
A functional Ig consists of two heterodimers each of which is
composed of a heavy and a light chain. Although there is increasing
knowledge about the events that govern the rearrangement of the genes
encoding each individual chain, only very limited information is
available about the mechanisms governing the pairing of variable heavy
(VH) and variable light (VL) chains.
Using a single cell PCR, we were able to obtain VH and V
chains from 144 individual human CD19+/IgM+ B
cells. Pairing of specific VH or V families was not
observed, nor was the length or the amino acid composition of the CDR3s
of VH and V chains in individual B cells similar.
Comparison of VH and V genes in B cells in which one or
both contained evidence of somatic hypermutation with those with no
mutations revealed a significant decrease in the mean length of the
VH CDR3. Moreover, there was a significant correlation
between the frequencies of mutations in VH and V gene
pairs in individual B cells. These results indicate that Ag-mediated
selection as opposed to VHDJH recombination or
subsequent Ig chain pairing tended to approximate the CDR3 lengths and
the frequency of mutations of VH and V in individual B
cells.
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