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Department of Microbiology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455
A small population of CD4+ OVA-specific TCR
transgenic T cells was tracked following the induction of peripheral
tolerance by soluble Ag to address whether functionally unresponsive,
or anergic T cells, persist in vivo for extended periods of time.
Although injection of OVA peptide in the absence of adjuvant caused a
transient expansion and deletion of the Ag-specific T cells, a
population that showed signs of prior activation persisted in the
lymphoid tissues for several months. These surviving OVA-specific T
cells had long-lasting, but reversible defects in their ability to
proliferate in lymph nodes and secrete IL-2 and TNF-
in vivo
following an antigenic challenge. These defects were not associated
with the production of Th2-type cytokines or the capacity to suppress
the clonal expansion of a bystander population of T cells present in
the same lymph nodes. Therefore, our results provide direct evidence
that a long-lived population of functionally impaired Ag-specific
CD4+ T cells is generated in vivo after exposure to soluble
Ag.
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