| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
*
Department of Biology, University of Oslo, Oslo, Norway;
Department of Pediatrics, School of Medicine, and
Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104; and
§
Department of Pediatrics, Stanford University Medical Center, Stanford, CA 94305
In APCs, MHC class II molecules (MHC class II) bind antigenic peptides after HLA-DM mediated removal of CLIP. To characterize intracellular sites of peptide loading in human B lymphoblastoid cell lines, we conducted immunoelectron microscopy studies with Abs recognizing MHC class II associated with CLIP or bound peptide, respectively, together with Abs to HLA-DM and endocytic markers. The distribution of these molecules indicates that peptide binding occurs in compartments with characteristics of normal late endosomes, and in compartments that show characteristics of late endosomes, but are not detectably accessed by endocytosed BSA-gold. The latter compartments may represent or give rise to recycling vesicles that deliver peptide-loaded class II molecules to the cell surface. In addition, we have compared cells in which HLA-DM and HLA-DR interaction is defective with cells in which this interaction is intact, and find that DM/DR interaction is not required for the proper localization of either molecule to peptide-loading compartments.
This article has been cited by other articles:
|
|
 |
|
  P. Chaturvedi, R. Hengeveld, M. A. Zechel, E. Lee-Chan, and B. Singh The functional role of class II-associated invariant chain peptide (CLIP) in its ability to variably modulate immune responses Int. Immunol., June 1, 2000; 12(6): 757 - 765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Krogsgaard, K. W. Wucherpfennig, B. Canella, B. E. Hansen, A. Svejgaard, J. Pyrdol, H. Ditzel, C. Raine, J. Engberg, and L. Fugger Visualization of Myelin Basic Protein (Mbp) T Cell Epitopes in Multiple Sclerosis Lesions Using a Monoclonal Antibody Specific for the Human Histocompatibility Leukocyte Antigen (Hla)-Dr2-Mbp 85-99 Complex J. Exp. Med., April 17, 2000; 191(8): 1395 - 1412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Simonsen, K. W. Pedersen, T. W. Nordeng, A. von der Lippe, E. Stang, E. O. Long, and O. Bakke Polarized Transport of MHC Class II Molecules in Madin-Darby Canine Kidney Cells Is Directed by a Leucine-Based Signal in the Cytoplasmic Tail of the {beta}-Chain J. Immunol., September 1, 1999; 163(5): 2540 - 2548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Busch, Z. Reich, D. M. Zaller, V. Sloan, and E. D. Mellins Secondary Structure Composition and pH-dependent Conformational Changes of Soluble Recombinant HLA-DM J. Biol. Chem., October 16, 1998; 273(42): 27557 - 27564. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
