The Journal of Immunology, Vol 159, Issue 8 4056-4063, Copyright © 1997 by American Association of Immunologists

ARTICLES

Modulation of antigen-induced B and T cell responses by antigen- specific IgE antibodies

A Oshiba, E Hamelmann, A Haczku, K Takeda, DH Conrad, H Kikutani and EW Gelfand
Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA.

Ag-specific IgE Abs not only mediate immediate hypersensitivity through mast cell activation, but also enhance in vitro Ag presentation and in vivo specific Ab responses in mice. To delineate the role of IgE Ab in the modulation of Ag-specific responses, spleen cells from OVA- sensitized BALB/C mice were cultured together with OVA-specific IgE (or IgG isotypes). OVA-dependent proliferative responses and anti-OVA IgE production were enhanced in the presence of anti-OVA IgE. A significant decrease in IFN-gamma secretion in OVA-stimulated cultures was observed in the presence of anti-OVA IgE, but no changes in IL-4 production were detected. Anti-OVA IgG isotypes or anti-TNP IgE showed no significant effect on any of these Ag-dependent responses. Addition of anti-CD23 Ab abolished these effects of anti-OVA IgE. Further, OVA-sensitized spleen cells from CD23-deficient mice responded to in vitro stimulation with OVA, but demonstrated no modulation by anti-OVA IgE. These results demonstrate that Ag-specific IgE not only augments Ag presentation and T cell proliferation, but also alters the pattern of cytokine production and increases specific IgE synthesis. These modulatory effects of Ag-specific IgE appear to be mediated by binding to Fc epsilon RII/CD23.

This article has been cited by other articles:

				K. Takeda, N. Miyahara, T. Kodama, C. Taube, A. Balhorn, A. Dakhama, K. Kitamura, A. Hirano, M. Tanimoto, and E. W. Gelfand S-carboxymethylcysteine normalises airway responsiveness in sensitised and challenged mice Eur. Respir. J., October 1, 2005; 26(4): 577 - 585. [Abstract] [Full Text] [PDF]

				N. Miyahara, K. Takeda, S. Miyahara, S. Matsubara, T. Koya, A. Joetham, E. Krishnan, A. Dakhama, B. Haribabu, and E. W. Gelfand Requirement for Leukotriene B4 Receptor 1 in Allergen-induced Airway Hyperresponsiveness Am. J. Respir. Crit. Care Med., July 15, 2005; 172(2): 161 - 167. [Abstract] [Full Text] [PDF]

				A. Joetham, K. Takeda, C. Taube, N. Miyahara, A. Kanehiro, A. Dakhama, and E. W. Gelfand Airway Hyperresponsiveness in the Absence of CD4+ T Cells after Primary but Not Secondary Challenge Am. J. Respir. Cell Mol. Biol., July 1, 2005; 33(1): 89 - 96. [Abstract] [Full Text] [PDF]

				E. W. Gelfand, A. Joetham, Z.-H. Cui, A. Balhorn, K. Takeda, C. Taube, and A. Dakhama Induction and Maintenance of Airway Responsiveness to Allergen Challenge Are Determined at the Age of Initial Sensitization J. Immunol., July 15, 2004; 173(2): 1298 - 1306. [Abstract] [Full Text] [PDF]

				C. Taube, Y.-H. Rha, K. Takeda, J.-W. Park, A. Joetham, A. Balhorn, A. Dakhama, P. C. Giclas, V. M. Holers, and E. W. Gelfand Inhibition of Complement Activation Decreases Airway Inflammation and Hyperresponsiveness Am. J. Respir. Crit. Care Med., December 1, 2003; 168(11): 1333 - 1341. [Abstract] [Full Text] [PDF]

				K. Takeda, N. Miyahara, Y.-H. Rha, C. Taube, E.-S. Yang, A. Joetham, T. Kodama, A. M. Balhorn, A. Dakhama, C. Duez, et al. Surfactant Protein D Regulates Airway Function and Allergic Inflammation through Modulation of Macrophage Function Am. J. Respir. Crit. Care Med., October 1, 2003; 168(7): 783 - 789. [Abstract] [Full Text] [PDF]

				E. Reali, J. W. Greiner, A. Corti, H. J. Gould, F. Bottazzoli, G. Paganelli, J. Schlom, and A. G. Siccardi IgEs Targeted on Tumor Cells: Therapeutic Activity and Potential in the Design of Tumor Vaccines Cancer Res., July 1, 2001; 61(14): 5517 - 5522. [Abstract] [Full Text] [PDF]

				S. Jilek, C. Barbey, F. Spertini, and B. Corthesy Antigen-Independent Suppression of the Allergic Immune Response to Bee Venom Phospholipase A2 by DNA Vaccination in CBA/J Mice J. Immunol., March 1, 2001; 166(5): 3612 - 3621. [Abstract] [Full Text] [PDF]

				C. M. Lloyd, J.-A. Gonzalo, T. Nguyen, T. Delaney, J. Tian, H. Oettgen, A. J. Coyle, and J.-C. Gutierrez-Ramos Resolution of Bronchial Hyperresponsiveness and Pulmonary Inflammation Is Associated with IL-3 and Tissue Leukocyte Apoptosis J. Immunol., February 1, 2001; 166(3): 2033 - 2040. [Abstract] [Full Text] [PDF]

				S. Gustavsson, S. Wernersson, and B. Heyman Restoration of the Antibody Response to IgE/Antigen Complexes in CD23-Deficient Mice by CD23+ Spleen or Bone Marrow Cells J. Immunol., April 15, 2000; 164(8): 3990 - 3995. [Abstract] [Full Text] [PDF]

				A. HACZKU, K. TAKEDA, E. HAMELMANN, J. LOADER, A. JOETHAM, I. REDAI, C. G. IRVIN, J. J. LEE, H. KIKUTANI, D. CONRAD, et al. CD23 Exhibits Negative Regulatory Effects on Allergic Sensitization and Airway Hyperresponsiveness Am. J. Respir. Crit. Care Med., March 1, 2000; 161(3): 952 - 960. [Abstract] [Full Text] [PDF]

				E. HAMELMANN, G. CIESLEWICZ, J. SCHWARZE, T. ISHIZUKA, A. JOETHAM, C. HEUSSER, and E. W. GELFAND Anti-interleukin 5 But Not Anti-IgE Prevents Airway Inflammation and Airway Hyperresponsiveness Am. J. Respir. Crit. Care Med., September 1, 1999; 160(3): 934 - 941. [Abstract] [Full Text]

				R. J. J. van Neerven, T. Wikborg, G. Lund, B. Jacobsen, A. Brinch-Nielsen, J. Arnved, and H. Ipsen Blocking Antibodies Induced by Specific Allergy Vaccination Prevent the Activation of CD4+ T Cells by Inhibiting Serum-IgE-Facilitated Allergen Presentation J. Immunol., September 1, 1999; 163(5): 2944 - 2952. [Abstract] [Full Text] [PDF]

				L. J. Rosenwasser and E. W. Gelfand Immunotherapy with Antigens and Epitopes . Pro or Con Am. J. Respir. Cell Mol. Biol., July 1, 1999; 21(1): 4 - 6. [Full Text]

				S. Gustavsson, S. Hjulstrom-Chomez, B.-M. Lidstrom, N. Ahlborg, R. Andersson, and B. Heyman Impaired Antibody Responses in H-2Ab Mice J. Immunol., August 15, 1998; 161(4): 1765 - 1771. [Abstract] [Full Text] [PDF]