The Journal of Immunology, Vol 159, Issue 8 3675-3679, Copyright © 1997 by American Association of Immunologists

CUTTING EDGE

A synthetic peptide administered with IL-12 elicits immunity to Listeria monocytogenes

MA Miller, MJ Skeen and HK Ziegler
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA.

IL-12 is a pivotal cytokine signal for the development of Th1-type cellular responses that are required for control of intracellular pathogens. We previously demonstrated that coinjection of IL-12 with heat-killed Listeria monocytogenes, which was not immunogenic when injected alone, elicited intense Ag-specific T cell responses that conferred protection against subsequent challenge with Listeria. Herein we describe the remarkable finding that a nonimmunogenic synthetic peptide corresponding to a dominant MHC class II (H-2k)-restricted listerial determinant, when coinjected i.p. with murine IL-12, elicited potent Ag-specific immune responses that conferred protective immunity against Listeria.

This article has been cited by other articles:

				M. M. Freeman and H. K. Ziegler Simultaneous Th1-Type Cytokine Expression Is a Signature of Peritoneal CD4+ Lymphocytes Responding to Infection with Listeria monocytogenes J. Immunol., July 1, 2005; 175(1): 394 - 403. [Abstract] [Full Text] [PDF]

				Z. Su, M.-F. Tam, D. Jankovic, and M. M. Stevenson Vaccination with Novel Immunostimulatory Adjuvants against Blood-Stage Malaria in Mice Infect. Immun., September 1, 2003; 71(9): 5178 - 5187. [Abstract] [Full Text] [PDF]

				S. S. Way, A. C. Borczuk, and M. B. Goldberg Thymic Independence of Adaptive Immunity to the Intracellular Pathogen Shigella flexneri Serotype 2a Infect. Immun., August 1, 1999; 67(8): 3970 - 3979. [Abstract] [Full Text] [PDF]

				L. R. Schopf, J. L. Bliss, L. M. Lavigne, C. L. Chung, S. F. Wolf, and J. P. Sypek Interleukin-12 Is Capable of Generating an Antigen-Specific Th1-Type Response in the Presence of an Ongoing Infection-Driven Th2-Type Response Infect. Immun., May 1, 1999; 67(5): 2166 - 2171. [Abstract] [Full Text] [PDF]

				Y. Tanabe, H. Xiong, T. Nomura, M. Arakawa, and M. Mitsuyama Induction of Protective T Cells against Listeria monocytogenes in Mice by Immunization with a Listeriolysin O-Negative Avirulent Strain of Bacteria and Liposome-Encapsulated Listeriolysin O Infect. Immun., February 1, 1999; 67(2): 568 - 575. [Abstract] [Full Text] [PDF]

				H. Su, R. Messer, W. Whitmire, E. Fischer, J. C. Portis, and H. D. Caldwell Vaccination against Chlamydial Genital Tract Infection after Immunization with Dendritic Cells Pulsed Ex Vivo with Nonviable Chlamydiae J. Exp. Med., September 7, 1998; 188(5): 809 - 818. [Abstract] [Full Text] [PDF]