| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 159, Issue 6 2741-2749, Copyright © 1997 by American Association of Immunologists
ARTICLES |
T Kanaitsuka, R Bortell, LA Stevens, J Moss, D Sardinha, TV Rajan, D Zipris, JP Mordes, DL Greiner and AA Rossini
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655, USA.
Several proteins with NAD+:arginine ADP-ribosyltransferase (ART) activity are expressed in T cells and affect their function. Rat T cells that express the ART designated RT6 are determinants of the expression of autoimmune diabetes. In the mouse, a 35-kDa ecto-ART modulates the proliferation and functional activity of CTL. Here we report on mouse ARTs designated Rt6-1 and Rt6-2 in BALB/c and C57BL/6 mice. mRNAs for Rt6-1 and Rt6-2 were found in spleen, thymus, and intestinal tissue of both strains, but Rt6-1 mRNA in C57BL/6 mice was detected only at low levels. Rt6-1 and Rt6-2 cDNAs from both strains were cloned and sequenced. Predicted amino acid sequences of Rt6-2 were identical in both strains, but there was an in-frame stop codon in the sequence of Rt6-1 in C57BL/6 mice not present in BALB/c mice. Recombinant C57BL/6 Rt6-2 and BALB/c Rt6-1 proteins expressed in COS1 cells exhibited ART activity and were documented to be glycosylphosphatidylinositol-linked membrane proteins. COS-1 cells transfected with a C57BL/6 Rt6-1 cDNA construct expressed a truncated protein consistent in size with that predicted by the presence of the stop codon. This approximately 21-kDa protein appeared not to be glycosylphosphatidylinositol linked to the cell surface and lacked ART activity. C57BL/6 Rt6-1 therefore appears to be a naturally occurring ART knockout. The expression of Rt6-1 and Rt6-2 mRNAs in lymphoid tissues suggests that these ARTs may regulate immune system functions. Expression of Rt6-2 or another redundant ART may compensate for the lack of enzymatically active Rt6-1 in C57BL/6 mice.
This article has been cited by other articles:
|
|
 |
|
  S. Hong, A. Brass, M. Seman, F. Haag, F. Koch-Nolte, and G. R. Dubyak Lipopolysaccharide, IFN-{gamma}, and IFN-beta Induce Expression of the Thiol-Sensitive ART2.1 Ecto-ADP-Ribosyltransferase in Murine Macrophages J. Immunol., November 1, 2007; 179(9): 6215 - 6227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Paone, L. A. Stevens, R. L. Levine, C. Bourgeois, W. K. Steagall, B. R. Gochuico, and J. Moss ADP-ribosyltransferase-specific Modification of Human Neutrophil Peptide-1 J. Biol. Chem., June 23, 2006; 281(25): 17054 - 17060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-X. Liu, O. Azhipa, S. Okamoto, S. Govindarajan, and G. Dennert Extracellular Nicotinamide Adenine Dinucleotide Induces T Cell Apoptosis In Vivo and In Vitro J. Immunol., November 1, 2001; 167(9): 4942 - 4947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Adriouch, W. Ohlrogge, F. Haag, F. Koch-Nolte, and M. Seman Rapid Induction of Naive T Cell Apoptosis by Ecto-Nicotinamide Adenine Dinucleotide: Requirement for Mono(ADP-Ribosyl)Transferase 2 and a Downstream Effector J. Immunol., July 1, 2001; 167(1): 196 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kahl, M. Nissen, R. Girisch, T. Duffy, E. H. Leiter, F. Haag, and F. Koch-Nolte Metalloprotease-Mediated Shedding of Enzymatically Active Mouse ecto-ADP-ribosyltransferase ART2.2 Upon T Cell Activation J. Immunol., October 15, 2000; 165(8): 4463 - 4469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Koch-Nolte, T. Duffy, M. Nissen, S. Kahl, N. Killeen, V. Ablamunits, F. Haag, and E. H. Leiter A New Monoclonal Antibody Detects a Developmentally Regulated Mouse Ecto-ADP-Ribosyltransferase on T Cells: Subset Distribution, Inbred Strain Variation, and Modulation Upon T Cell Activation J. Immunol., December 1, 1999; 163(11): 6014 - 6022. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
