The Journal of Immunology, Vol 159, Issue 4 1853-1859, Copyright © 1997 by American Association of Immunologists

ARTICLES

Yersinia invasin, a bacterial beta1-integrin ligand, is a potent inducer of lymphocyte motility and migration to collagen type IV and fibronectin

I Arencibia, NC Suarez, H Wolf-Watz and KG Sundqvist
Department of Clinical Immunology, University of Umea, Sweden.

The Yersinia pseudotuberculosis invasin protein was found to be a potent inducer of pseudopodia formation and chemotactic and haptotactic migration in human T lymphocytes. Checkerboard analysis confirmed that migration was directional. The Yersinia invasin triggered migration of otherwise poorly migratory normal T cells on fibronectin and in particular on collagen type IV, and augmented the migration of leukemic T cell lines on these components. Invasin-induced lymphocyte migration was inhibited by staurosporin that selectively prevented pseudopodia formation but, noteworthy, augmented adhesion. The motogenic and attractant properties of invasin (Inv) were mediated via beta1- integrins, as shown by lack of effect of Inv on the motility of a beta1- integrin-negative lymphoid cell line and inhibition of invasin-induced lymphocyte motility by anti-beta1 Abs. Inv was markedly more effective than the extracellular matrix components fibronectin, collagen type IV, and laminin, which also interact with lymphocyte beta1-integrins, with respect to induction of pseudopodia, chemotaxis, and haptotaxis. Thus, Yersinia invasin is a model ligand for induction of lymphocyte motility via beta1-integrins. The extraordinary capacity of Inv to trigger and guide T lymphocyte motility and potentiate lymphocyte migration to extracellular matrix components may be of pathogenetic significance for the movement of lymphocytes to extraintestinal sites secondary to Yersinia infection.

This article has been cited by other articles:

				R. E. Rojas, J. J. Thomas, A. J. Gehring, P. J. Hill, J. T. Belisle, C. V. Harding, and W. H. Boom Phosphatidylinositol Mannoside from Mycobacterium tuberculosis Binds {alpha}5beta1 Integrin (VLA-5) on CD4+ T Cells and Induces Adhesion to Fibronectin. J. Immunol., September 1, 2006; 177(5): 2959 - 2968. [Abstract] [Full Text] [PDF]

				B. Segerman, P. Holmfeldt, J. Morabito, L. Cassimeris, and M. Gullberg Autonomous and phosphorylation-responsive microtubule-regulating activities of the N-terminus of Op18/stathmin J. Cell Sci., January 1, 2003; 116(1): 197 - 205. [Abstract] [Full Text] [PDF]

				J. Vadnais, G. Nault, Z. Daher, M. Amraei, Y. Dodier, I. R. Nabi, and J. Noel Autocrine Activation of the Hepatocyte Growth Factor Receptor/Met Tyrosine Kinase Induces Tumor Cell Motility by Regulating Pseudopodial Protrusion J. Biol. Chem., December 6, 2002; 277(50): 48342 - 48350. [Abstract] [Full Text] [PDF]

				S. Franitza, R. Hershkoviz, N.'a. Kam, N. Lichtenstein, G. G. Vaday, R. Alon, and O. Lider TNF-{alpha} Associated with Extracellular Matrix Fibronectin Provides a Stop Signal for Chemotactically Migrating T Cells J. Immunol., September 1, 2000; 165(5): 2738 - 2747. [Abstract] [Full Text] [PDF]

				T. Yao, J. Mecsas, J. I. Healy, S. Falkow, and Y.-h. Chien Suppression of T and B Lymphocyte Activation by a Yersinia pseudotuberculosis Virulence Factor, YopH J. Exp. Med., November 1, 1999; 190(9): 1343 - 1350. [Abstract] [Full Text] [PDF]