The Journal of Immunology, Vol 159, Issue 4 1820-1827, Copyright © 1997 by American Association of Immunologists

ARTICLES

Salmonella typhimurium infections in mice deficient in interleukin-4 production: role of IL-4 in infection-associated pathology

P Everest, J Allen, A Papakonstantinopoulou, P Mastroeni, M Roberts and G Dougan
Department of Biochemistry, Imperial College of Science Technology, and Medicine, London, United Kingdom.

Mice harboring mutations in the IL-4 gene (IL-4(-/-)) were infected with a range of Salmonella typhimurium HWSH derivatives using different routes of infection. Compared with IL-4(+/+) mice, IL-4(-/-) mice exhibited a delayed time to death following infection with wild-type S. typhimurium HWSH. Groups of IL-4(+/+) mice infected with S. typhimurium HWSH purE, a less virulent derivative, showed sporadic deaths and harbored micro- or macroabscesses in their tissues, particularly associated with the liver. However, IL-4(-/-) mice infected with similar doses of S. typhimurium HWSH purE bacteria were resistant to killing and failed to develop detectable abscesses. Abscess formation in IL-4(-/-) mice could be induced by i.v. administration of rIL-4 during the S. typhimurium HWSH purE infection. The immune response in both IL-4(-/-) and IL-4(+/+) mice was of the Th1-type. Viable salmonella bacteria could be found associated with abscesses. Both IL- 4(-/-) and IL-4(+/+) mice were resistant to killing by S. typhimurium aroA.

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