| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 159, Issue 10 4848-4856, Copyright © 1997 by American Association of Immunologists
ARTICLES |
C Penit and F Vasseur
INSERM U345, Institut Necker, Paris, France. penit@infobiogen.fr
A small population of DNA-synthesizing mature thymocytes could be defined by analyzing cell surface markers and 5-bromo-2'-deoxyuridine (BrdUrd) labeling by four-color cytofluorometry. These cells have a completely mature phenotype (CD4- CD8+ or CD4+ CD8- TCR(high), HSA-, Qa- 2(high)) and expand only weakly after BrdUrd incorporation. They recovered immediately in total number and in DNA synthesis rate after treatment with the antimitotic drug demecolcin, thus much faster than immature CD4+ CD8+ thymocytes. These data demonstrate the existence of a late intrathymic expansion phase, independent of that of developing CD4+ CD8+ immature cells, and involving phenotypically mature cells renewed each day. In mixed chimeras prepared by transfer of bone marrow and lymph node cells into RAG-2(-/-) mice, all cycling mature thymocytes were bone marrow derived. They are thus produced in situ and do not correspond to peripheral T cells reentering the thymus. Double FITC/BrdUrd detection showed that a high proportion (10-20%) of recent thymic emigrants were BrdUrd+ just postcycling cells and that around 50% of cycling mature thymocytes are just ready to emigrate to the periphery in the few hours after DNA synthesis. The late intrathymic expansion phase demonstrated here increases the daily thymic cell export by at least 30%. It could play a role in the adjustment of the T cell repertoire before emigration and in the regulation of the thymic cell output into the peripheral T cell pool.
This article has been cited by other articles:
|
|
 |
|
  Y.-W. Chu, S. Schmitz, B. Choudhury, W. Telford, V. Kapoor, S. Garfield, D. Howe, and R. E. Gress Exogenous insulin-like growth factor 1 enhances thymopoiesis predominantly through thymic epithelial cell expansion Blood, October 1, 2008; 112(7): 2836 - 2846. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Thomas-Vaslin, H. K. Altes, R. J. de Boer, and D. Klatzmann Comprehensive Assessment and Mathematical Modeling of T Cell Population Dynamics and Homeostasis J. Immunol., February 15, 2008; 180(4): 2240 - 2250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Jin, W. Wang, J.-Y. Yao, Y.-B. Zhou, X.-P. Qian, J. Zhang, Y. Zhang, and W.-F. Chen Characterization of the In Vivo Dynamics of Medullary CD4+CD8- Thymocyte Development J. Immunol., February 15, 2008; 180(4): 2256 - 2263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. McCaughtry, M. S. Wilken, and K. A. Hogquist Thymic emigration revisited J. Exp. Med., October 29, 2007; 204(11): 2513 - 2520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Uldrich, S. P. Berzins, M. A. Malin, P. Bouillet, A. Strasser, M. J. Smyth, R. L. Boyd, and D. I. Godfrey Antigen challenge inhibits thymic emigration. J. Immunol., April 15, 2006; 176(8): 4553 - 4561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ramanathan, J. Gagnon, C. Leblanc, R. Rottapel, and S. Ilangumaran Suppressor of Cytokine Signaling 1 Stringently Regulates Distinct Functions of IL-7 and IL-15 In Vivo during T Lymphocyte Development and Homeostasis J. Immunol., April 1, 2006; 176(7): 4029 - 4041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Berzins, F. W. McNab, C. M. Jones, M. J. Smyth, and D. I. Godfrey Long-Term Retention of Mature NK1.1+ NKT Cells in the Thymus J. Immunol., April 1, 2006; 176(7): 4059 - 4065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Penkov, P. Di Rosa, L. Fernandez Diaz, V. Basso, E. Ferretti, F. Grassi, A. Mondino, and F. Blasi Involvement of Prep1 in the {alpha}{beta} T-Cell Receptor T-Lymphocytic Potential of Hematopoietic Precursors Mol. Cell. Biol., December 15, 2005; 25(24): 10768 - 10781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Vianello, P. Kraft, Y. T. Mok, W. K. Hart, N. White, and M. C. Poznansky A CXCR4-Dependent Chemorepellent Signal Contributes to the Emigration of Mature Single-Positive CD4 Cells from the Fetal Thymus J. Immunol., October 15, 2005; 175(8): 5115 - 5125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. S. P. Heng, G. L. Goldberg, D. H. D. Gray, J. S. Sutherland, A. P. Chidgey, and R. L. Boyd Effects of Castration on Thymocyte Development in Two Different Models of Thymic Involution J. Immunol., September 1, 2005; 175(5): 2982 - 2993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-W. Chu, S. A. Memon, S. O. Sharrow, F. T. Hakim, M. Eckhaus, P. J. Lucas, and R. E. Gress Exogenous IL-7 increases recent thymic emigrants in peripheral lymphoid tissue without enhanced thymic function Blood, August 15, 2004; 104(4): 1110 - 1119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Krenger, H. Schmidlin, G. Cavadini, and G. A. Hollander On the Relevance of TCR Rearrangement Circles as Molecular Markers for Thymic Output during Experimental Graft-versus-Host Disease J. Immunol., June 15, 2004; 172(12): 7359 - 7367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kwan and N. Killeen CCR7 Directs the Migration of Thymocytes into the Thymic Medulla J. Immunol., April 1, 2004; 172(7): 3999 - 4007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hue, R. C. Monteiro, S. Berrih-Aknin, and S. Caillat-Zucman Potential Role of NKG2D/MHC Class I-Related Chain A Interaction in Intrathymic Maturation of Single-Positive CD8 T Cells J. Immunol., August 15, 2003; 171(4): 1909 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Reinhardt, D. C. Bullard, C. T. Weaver, and M. K. Jenkins Preferential Accumulation of Antigen-specific Effector CD4 T Cells at an Antigen Injection Site Involves CD62E-dependent Migration but Not Local Proliferation J. Exp. Med., March 17, 2003; 197(6): 751 - 762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Berzins, E. S. Venanzi, C. Benoist, and D. Mathis T-Cell Compartments of Prediabetic NOD Mice Diabetes, February 1, 2003; 52(2): 327 - 334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Boyer, G. Marodon, J. L. Cohen, L. Lejeune, T. Irinopoulou, R. Liblau, P. Bruneval, and D. Klatzmann Human CD4 Expression at the Late Single-Positive Stage of Thymic Development Supports T Cell Maturation and Peripheral Export in CD4-Deficient Mice J. Immunol., October 15, 2002; 169(8): 4347 - 4353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rossi, B. R. Blazar, C. L. Farrell, D. M. Danilenko, D. L. Lacey, K. I. Weinberg, W. Krenger, and G. A. Hollander Keratinocyte growth factor preserves normal thymopoiesis and thymic microenvironment during experimental graft-versus-host disease Blood, June 28, 2002; 100(2): 682 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Le Campion, C. Bourgeois, F. Lambolez, B. Martin, S. Leaument, N. Dautigny, C. Tanchot, C. Penit, and B. Lucas Naive T cells proliferate strongly in neonatal mice in response to self-peptide/self-MHC complexes PNAS, April 2, 2002; 99(7): 4538 - 4543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Le Campion, B. Lucas, N. Dautigny, S. Leaument, F. Vasseur, and C. Penit Quantitative and Qualitative Adjustment of Thymic T Cell Production by Clonal Expansion of Premigrant Thymocytes J. Immunol., February 15, 2002; 168(4): 1664 - 1671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hassan and D. J. Reen Human Recent Thymic Emigrants-Identification, Expansion, And Survival Characteristics J. Immunol., August 15, 2001; 167(4): 1970 - 1976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tian, J. Zhang, L. Gao, X. P. Qian, and W.-F. Chen Heterogeneity within medullary-type TCR{{alpha}}{beta}+CD3+CD4-CD8+ thymocytes in normal mouse thymus Int. Immunol., March 1, 2001; 13(3): 313 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hayashi, W. Natsume, T. Watanabe, N. Abe, N. Iwai, H. Okada, Y. Ito, M. Asano, Y. Iwakura, S. Habu, et al. Diminution of the AML1 Transcription Factor Function Causes Differential Effects on the Fates of CD4 and CD8 Single-Positive T Cells J. Immunol., December 15, 2000; 165(12): 6816 - 6824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Krenger, S. Rossi, L. Piali, and G. A. Hollander Thymic atrophy in murine acute graft-versus-host disease is effected by impaired cell cycle progression of host pro-T and pre-T cells Blood, July 1, 2000; 96(1): 347 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Detours, R. Mehr, and A. S. Perelson Deriving Quantitative Constraints on T Cell Selection from Data on the Mature T Cell Repertoire J. Immunol., January 1, 2000; 164(1): 121 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Dyall and J. Nikolic-Zugic The Final Maturation of At Least Some Single-positive CD4hi Thymocytes Does Not Require T Cell Receptor–Major Histocompatibility Complex Contact J. Exp. Med., September 20, 1999; 190(6): 757 - 764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Berzins, G. M. Davey, E. S. Randle-Barrett, M. A. Malin, B. J. Classon, S. Fraser, and R. L. Boyd Thymic Shared Antigen-2: A Novel Cell Surface Marker Associated with T Cell Differentiation and Activation J. Immunol., May 1, 1999; 162(9): 5119 - 5126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Vasseur, A. Le Campion, J. H. Pavlovitch, and C. Penit Distribution of Cycling T Lymphocytes in Blood and Lymphoid Organs During Immune Responses J. Immunol., May 1, 1999; 162(9): 5164 - 5172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Dautigny, A. Le Campion, and B. Lucas Timing and Casting for Actors of Thymic Negative Selection J. Immunol., February 1, 1999; 162(3): 1294 - 1302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.P. Berzins, R.L. Boyd, and J.F.A.P. Miller The Role of the Thymus and Recent Thymic Migrants in the Maintenance of the Adult Peripheral Lymphocyte Pool J. Exp. Med., June 1, 1998; 187(11): 1839 - 1848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Le Campion, C. Bourgeois, F. Lambolez, B. Martin, S. Leaument, N. Dautigny, C. Tanchot, C. Penit, and B. Lucas Naive T cells proliferate strongly in neonatal mice in response to self-peptide/self-MHC complexes PNAS, April 2, 2002; 99(7): 4538 - 4543. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
