| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 158, Issue 7 3215-3220, Copyright © 1997 by American Association of Immunologists
ARTICLES |
N Weng, BL Levine, CH June and RJ Hodes
Experimental Immunology Branch, National Cancer Institute, Bethesda, MD 20892, USA. wengn@DC10a.nci.nih.gov
Telomeres are unique DNA-protein complexes at the terminals of chromosomes that appear to play a critical role in protecting chromosomal integrity and in maintaining cellular replicative potential. Telomerase is a ribonuclear protein that is capable of elongating telomeres by the addition of telomeric hexanucleotide repeats and therefore contributing to the capacity for cell replication. Telomerase activity is expressed in human germline cells and malignant cells, and it has recently been demonstrated that telomerase activity is highly regulated in normal lymphocytes at specific stages of development and activation. However, these studies have not elucidated whether telomerase activity is regulated at the level of specific gene expression or whether the regulation of telomerase RNA template (hTR) and/or protein components contributes to the regulation of telomerase activity in normal somatic cells. To characterize at a molecular level the regulation of telomerase expression in human T lymphocytes, we analyzed the expression of hTR during lineage development and after in vitro activation. It was found that hTR is expressed in subsets of thymocytes with strong telomerase activity at levels that are consistently higher (1.5 times; p < 0.01) than those found in peripheral blood resting T cells. In addition, hTR is up-regulated two- to fivefold in peripheral blood naive and memory CD4+ T cells after in vitro activation with anti-CD3 plus anti-CD28. These results establish that hTR expression is regulated in normal human T cells during lineage development and after activation, and indicate that regulation of hTR expression may contribute to the regulation of telomerase activity in normal lymphoid cells.
This article has been cited by other articles:
|
|
 |
|
  Y. Yang, J. An, and N.-p. Weng Telomerase Is Involved in IL-7-Mediated Differential Survival of Naive and Memory CD4+ T Cells J. Immunol., March 15, 2008; 180(6): 3775 - 3781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Kilpatrick, T. Rickabaugh, L. E. Hultin, P. Hultin, M. A. Hausner, R. Detels, J. Phair, and B. D. Jamieson Homeostasis of the Naive CD4+ T Cell Compartment during Aging J. Immunol., February 1, 2008; 180(3): 1499 - 1507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Plunkett, O. Franzese, H. M. Finney, J. M. Fletcher, L. L. Belaramani, M. Salmon, I. Dokal, D. Webster, A. D. G. Lawson, and A. N. Akbar The Loss of Telomerase Activity in Highly Differentiated CD8+CD28-CD27- T Cells Is Associated with Decreased Akt (Ser473) Phosphorylation J. Immunol., June 15, 2007; 178(12): 7710 - 7719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Roth, G. M. Baerlocher, M. Schertzer, E. Chavez, U. Duhrsen, and P. M. Lansdorp Telomere loss, senescence, and genetic instability in CD4+ T lymphocytes overexpressing hTERT Blood, July 1, 2005; 106(1): 43 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kawauchi, K. Ihjima, and O. Yamada IL-2 Increases Human Telomerase Reverse Transcriptase Activity Transcriptionally and Posttranslationally through Phosphatidylinositol 3'-Kinase/Akt, Heat Shock Protein 90, and Mammalian Target of Rapamycin in Transformed NK Cells J. Immunol., May 1, 2005; 174(9): 5261 - 5269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Matsumura-Arioka, K. Ohtani, T. Hara, R. Iwanaga, and M. Nakamura Identification of two distinct elements mediating activation of telomerase (hTERT) gene expression in association with cell growth in human T cells Int. Immunol., February 1, 2005; 17(2): 207 - 215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Kurosaka, J. Yasuda, K. Yoshida, T. Yokoyama, Y. Ozawa, Y. Obayashi, I. Kingetsu, S. Saito, and A. Yamada Telomerase activity and telomere length of peripheral blood mononuclear cells in SLE patients Lupus, August 1, 2003; 12(8): 591 - 599. [Abstract] [PDF]
|
|
|
|
|
|
A. Roth, H. Yssel, J. Pene, E. A. Chavez, M. Schertzer, P. M. Lansdorp, H. Spits, and R. M. Luiten Telomerase levels control the lifespan of human T lymphocytes Blood, August 1, 2003; 102(3): 849 - 857. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D KUROSAKA, Y OZAWA, J YASUDA, T YOKOYAMA, A YAMADA, M AKIYAMA, S SAITO, and N TAJIMA Telomerase activity in peripheral blood mononuclear cells from patients with SLE Ann Rheum Dis, December 1, 2001; 60(12): 1158 - 1159. [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Londono-Vallejo, H. DerSarkissian, L. Cazes, and G. Thomas Differences in telomere length between homologous chromosomes in humans Nucleic Acids Res., August 1, 2001; 29(15): 3164 - 3171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Lin, S. Lim, M. A. Viani, M. Sapp, and M. S. Lim Down-Regulation of Telomerase Activity in Malignant Lymphomas by Radiation and Chemotherapeutic Agents Am. J. Pathol., August 1, 2001; 159(2): 711 - 719. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Seki, T. Suda, Y. Aoyagi, S. Sugawara, M. Natsui, H. Motoyama, Y. Shirai, T. Sekine, H. Kawai, Y. Mita, et al. Diagnosis of Pancreatic Adenocarcinoma by Detection of Human Telomerase Reverse Transcriptase Messenger RNA in Pancreatic Juice with Sample Qualification Clin. Cancer Res., July 1, 2001; 7(7): 1976 - 1981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Migliaccio, M. Amacker, T. Just, P. Reichenbach, D. Valmori, J.-C. Cerottini, P. Romero, and M. Nabholz Ectopic Human Telomerase Catalytic Subunit Expression Maintains Telomere Length But Is Not Sufficient for CD8+ T Lymphocyte Immortalization J. Immunol., November 1, 2000; 165(9): 4978 - 4984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hooijberg, J. J. Ruizendaal, P. J. F. Snijders, E. W. M. Kueter, J. M. M. Walboomers, and H. Spits Immortalization of Human CD8+ T Cell Clones by Ectopic Expression of Telomerase Reverse Transcriptase J. Immunol., October 15, 2000; 165(8): 4239 - 4245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wu, N. Higashi, E. R. Hansen, M. Lund, K. Bang, and K. Thestrup-Pedersen Telomerase Activity Is Increased and Telomere Length Shortened in T Cells from Blood of Patients with Atopic Dermatitis and Psoriasis J. Immunol., October 15, 2000; 165(8): 4742 - 4747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Finnon, A. R.J. Silver, and S. D. Bouffler Upregulation of telomerase activity by X-irradiation in mouse leukaemia cells is independent of Tert, Terc, Tnks and Myc transcription Carcinogenesis, April 1, 2000; 21(4): 573 - 578. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Rufer, T. H. Brummendorf, S. Kolvraa, C. Bischoff, K. Christensen, L. Wadsworth, M. Schulzer, and P. M. Lansdorp Telomere Fluorescence Measurements in Granulocytes and T Lymphocyte Subsets Point to a High Turnover of Hematopoietic Stem Cells and Memory T Cells in Early Childhood J. Exp. Med., July 19, 1999; 190(2): 157 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Liu, M. M. Schoonmaker, B. L. Levine, C. H. June, R. J. Hodes, and N.-p. Weng Constitutive and regulated expression of telomerase reverse transcriptase (hTERT) in human lymphocytes PNAS, April 27, 1999; 96(9): 5147 - 5152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yajima, A. Yagihashi, H. Kameshima, D. Kobayashi, D. Furuya, K. Hirata, and N. Watanabe Quantitative reverse transcription-PCR assay of the RNA component of human telomerase using the TaqMan fluorogenic detection system Clin. Chem., December 1, 1998; 44(12): 2441 - 2445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Vieira, D. Soares, N. J. Borthwick, M. K. Maini, G. Janossy, M. Salmon, and A. N. Akbar IL-7-Dependent Extrathymic Expansion of CD45RA+ T Cells Enables Preservation of a Naive Repertoire J. Immunol., December 1, 1998; 161(11): 5909 - 5917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. De Boer and A. J. Noest T Cell Renewal Rates, Telomerase, and Telomere Length Shortening J. Immunol., June 15, 1998; 160(12): 5832 - 5837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N.-p. Weng, L. Granger, and R. J. Hodes Telomere lengthening and telomerase activation during human B cell differentiation PNAS, September 30, 1997; 94(20): 10827 - 10832. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
