The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Griffin, J. P.
Right arrow Articles by Harding, C. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Griffin, J. P.
Right arrow Articles by Harding, C. V.

The Journal of Immunology, Vol 158, Issue 4 1523-1532, Copyright © 1997 by American Association of Immunologists

ARTICLES

Early endosomes and a late endocytic compartment generate different peptide-class II MHC complexes via distinct processing mechanisms

JP Griffin, R Chu and CV Harding
Department of Pathology and Cell Biology Program, Case Western Reserve University, Cleveland, OH 44106, USA.

Class II MHC Ag-processing compartments and mechanisms were compared for four antigenic epitopes from hen egg lysozyme (HEL) and RNase. T cell assays on subcellular fractions of Ag-pulsed macrophages detected the initial appearance of HEL-(48-61):I-Ak, HEL-(34-45):I-Ak, and RNase- (90-105):I-Ek complexes in a high density late endocytic compartment. In contrast, RNase-(42-56):I-Ak complexes never appeared in high density compartments, but were rapidly generated in low density endosomes. This early endosomal processing mechanism was 1) chloroquine inhibitable; 2) less sensitive than the late endocytic mechanism to 20 degrees C inhibition; 3) partially resistant to depletion of nascent class II MHC molecules with brefeldin A, suggesting some use of recycled class II MHC molecules, whereas the late endocytic processing mechanism was blocked by brefeldin A; and 4) involved in the processing of a DM-independent complex (RNase-(42-56):I-Ak). Thus, distinct processing compartments and mechanisms are identified for different epitopes even within a single Ag.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Belizaire and E. R. Unanue
Targeting proteins to distinct subcellular compartments reveals unique requirements for MHC class I and II presentation
PNAS, October 13, 2009; 106(41): 17463 - 17468.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Knorr, C. Karacsonyi, and R. Lindner
Endocytosis of MHC molecules by distinct membrane rafts
J. Cell Sci., May 15, 2009; 122(10): 1584 - 1594.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Robila, M. Ostankovitch, M. L. Altrich-VanLith, A. C. Theos, S. Drover, M. S. Marks, N. Restifo, and V. H. Engelhard
MHC Class II Presentation of gp100 Epitopes in Melanoma Cells Requires the Function of Conventional Endosomes and Is Influenced by Melanosomes
J. Immunol., December 1, 2008; 181(11): 7843 - 7852.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Mimura, Y. Mimura-Kimura, K. Doores, D. Golgher, B. G. Davis, R. A. Dwek, P. M. Rudd, and T. Elliott
Folding of an MHC class II-restricted tumor antigen controls its antigenicity via MHC-guided processing
PNAS, April 3, 2007; 104(14): 5983 - 5988.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. B. Lovitch, Z. Pu, and E. R. Unanue
Amino-Terminal Flanking Residues Determine the Conformation of a Peptide-Class II MHC Complex.
J. Immunol., March 1, 2006; 176(5): 2958 - 2968.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
N. R. Hegde, C. Dunn, D. M. Lewinsohn, M. A. Jarvis, J. A. Nelson, and D. C. Johnson
Endogenous human cytomegalovirus gB is presented efficiently by MHC class II molecules to CD4+ CTL
J. Exp. Med., October 17, 2005; 202(8): 1109 - 1119.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Dugast, H. Toussaint, C. Dousset, and P. Benaroch
AP2 Clathrin Adaptor Complex, but Not AP1, Controls the Access of the Major Histocompatibility Complex (MHC) Class II to Endosomes
J. Biol. Chem., May 20, 2005; 280(20): 19656 - 19664.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
E. Klein, S. Koch, B. Borm, J. Neumann, V. Herzog, N. Koch, and T. Bieber
CD83 localization in a recycling compartment of immature human monocyte-derived dendritic cells
Int. Immunol., April 1, 2005; 17(4): 477 - 487.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Sinnathamby, M. Maric, P. Cresswell, and L. C. Eisenlohr
Differential Requirements for Endosomal Reduction in the Presentation of Two H2-Ed-Restricted Epitopes from Influenza Hemagglutinin
J. Immunol., June 1, 2004; 172(11): 6607 - 6614.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. A. Musson, N. Walker, H. Flick-Smith, E. D. Williamson, and J. H. Robinson
Differential Processing of CD4 T-cell Epitopes from the Protective Antigen of Bacillus anthracis
J. Biol. Chem., December 26, 2003; 278(52): 52425 - 52431.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Sinnathamby and L. C. Eisenlohr
Presentation by Recycling MHC Class II Molecules of an Influenza Hemagglutinin-Derived Epitope That Is Revealed in the Early Endosome by Acidification
J. Immunol., April 1, 2003; 170(7): 3504 - 3513.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
E. M. Bertram, R. G. Hawley, and T. H. Watts
Overexpression of rab7 enhances the kinetics of antigen processing and presentation with MHC class II molecules in B cells
Int. Immunol., March 1, 2002; 14(3): 309 - 318.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
L. Ramachandra, E. Noss, W. H. Boom, and C. V. Harding
Processing of Mycobacterium tuberculosis Antigen 85B Involves Intraphagosomal Formation of Peptide-Major Histocompatibility Complex II Complexes and Is Inhibited by Live Bacilli that Decrease Phagosome Maturation
J. Exp. Med., November 12, 2001; 194(10): 1421 - 1432.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Qi and S. Ostrand-Rosenberg
H2-O Inhibits Presentation of Bacterial Superantigens, but Not Endogenous Self Antigens
J. Immunol., August 1, 2001; 167(3): 1371 - 1378.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. van Lith, M. van Ham, A. Griekspoor, E. Tjin, D. Verwoerd, J. Calafat, H. Janssen, E. Reits, L. Pastoors, and J. Neefjes
Regulation of MHC Class II Antigen Presentation by Sorting of Recycling HLA-DM/DO and Class II within the Multivesicular Body
J. Immunol., July 15, 2001; 167(2): 884 - 892.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Askew, R. S. Chu, A. M. Krieg, and C. V. Harding
CpG DNA Induces Maturation of Dendritic Cells with Distinct Effects on Nascent and Recycling MHC-II Antigen-Processing Mechanisms
J. Immunol., December 15, 2000; 165(12): 6889 - 6895.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. M. Hiltbold, A. M. Vlad, P. Ciborowski, S. C. Watkins, and O. J. Finn
The Mechanism of Unresponsiveness to Circulating Tumor Antigen MUC1 Is a Block in Intracellular Sorting and Processing by Dendritic Cells
J. Immunol., October 1, 2000; 165(7): 3730 - 3741.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Ramachandra and C. V. Harding
Phagosomes Acquire Nascent and Recycling Class II MHC Molecules but Primarily Use Nascent Molecules in Phagocytic Antigen Processing
J. Immunol., May 15, 2000; 164(10): 5103 - 5112.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
J. D. Lich, J. F. Elliott, and J. S. Blum
Cytoplasmic Processing Is a Prerequisite for Presentation of an Endogenous Antigen by Major Histocompatibility Complex Class II Proteins
J. Exp. Med., May 1, 2000; 191(9): 1513 - 1524.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Aiello, M. Noris, G. Piccinini, S. Tomasoni, F. Casiraghi, S. Bonazzola, M. Mister, M. H. Sayegh, and G. Remuzzi
Thymic Dendritic Cells Express Inducible Nitric Oxide Synthase and Generate Nitric Oxide in Response to Self- and Alloantigens
J. Immunol., May 1, 2000; 164(9): 4649 - 4658.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
M. van Ham, M. van Lith, B. Lillemeier, E. Tjin, U. Gruneberg, D. Rahman, L. Pastoors, K. van Meijgaarden, C. Roucard, J. Trowsdale, et al.
Modulation of the Major Histocompatibility Complex Class II-Associated Peptide Repertoire by Human Histocompatibility Leukocyte Antigen (Hla)-Do
J. Exp. Med., April 3, 2000; 191(7): 1127 - 1136.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
G Ramm, L Pond, C Watts, and W Stoorvogel
Clathrin-coated lattices and buds on MHC class II compartments do not selectively recruit mature MHC-II
J. Cell Sci., January 1, 2000; 113(2): 303 - 313.
[Abstract] [PDF]

Home page
 Int ImmunolHome page
P. Guermonprez, R. Lo-Man, C. Sedlik, M.-J. Rojas, R. J. Poljak, and C. Leclerc
mAb against hen egg-white lysozyme regulate its presentation to CD4+ T cells
Int. Immunol., November 1, 1999; 11(11): 1863 - 1872.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. L. McCool, C. V. Harding, N. S. Greenspan, and J. R. Schreiber
B- and T-Cell Immune Responses to Pneumococcal Conjugate Vaccines: Divergence between Carrier- and Polysaccharide-Specific Immunogenicity
Infect. Immun., September 1, 1999; 67(9): 4862 - 4869.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. S. Chu, D. Askew, E. H. Noss, A. Tobian, A. M. Krieg, and C. V. Harding
CpG Oligodeoxynucleotides Down-Regulate Macrophage Class II MHC Antigen Processing
J. Immunol., August 1, 1999; 163(3): 1188 - 1194.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Reijonen, J. F. Elliott, P. van Endert, and G. Nepom
Differential Presentation of Glutamic Acid Decarboxylase 65 (GAD65) T Cell Epitopes Among HLA-DRB1*0401-Positive Individuals
J. Immunol., August 1, 1999; 163(3): 1674 - 1681.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. C. Cheng, C. R. Steele, L. Gu, W. Song, and S. K. Pierce
MHC Class II Antigen Processing in B Cells: Accelerated Intracellular Targeting of Antigens
J. Immunol., June 15, 1999; 162(12): 7171 - 7180.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Ramachandra, R. Song, and C. V. Harding
Phagosomes Are Fully Competent Antigen-Processing Organelles That Mediate the Formation of Peptide:Class II MHC Complexes
J. Immunol., March 15, 1999; 162(6): 3263 - 3272.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
F. Forquet, N. Barois, P. Machy, J. Trucy, V. S. Zimmermann, L. Leserman, and J. Davoust
Presentation of Antigens Internalized Through the B Cell Receptor Requires Newly Synthesized MHC Class II Molecules
J. Immunol., March 15, 1999; 162(6): 3408 - 3416.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
D. A. Lazzarino, P. Blier, and I. Mellman
The Monomeric Guanosine Triphosphatase rab4 Controls an Essential Step on the Pathway of Receptor-mediated Antigen Processing in B Cells
J. Exp. Med., November 16, 1998; 188(10): 1769 - 1774.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Thery, V. Brachet, A. Regnault, M. Rescigno, P. Ricciardi-Castagnoli, C. Bonnerot, and S. Amigorena
MHC Class II Transport from Lysosomal Compartments to the Cell Surface Is Determined by Stable Peptide Binding, But Not by the Cytosolic Domains of the {alpha}- and {beta}-Chains
J. Immunol., September 1, 1998; 161(5): 2106 - 2113.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. A. Chianese-Bullock, H. I. Russell, C. Moller, W. Gerhard, J. J. Monaco, and L. C. Eisenlohr
Antigen Processing of Two H2-IEd-Restricted Epitopes Is Differentially Influenced by the Structural Changes in a Viral Glycoprotein
J. Immunol., August 15, 1998; 161(4): 1599 - 1607.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. A. Delvig and J. H. Robinson
Two T Cell Epitopes from the M5 Protein of Viable Streptococcus pyogenes Engage Different Pathways of Bacterial Antigen Processing in Mouse Macrophages
J. Immunol., June 1, 1998; 160(11): 5267 - 5272.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. K. Bikoff, G. Kenty, and L. Van Kaer
Distinct Peptide Loading Pathways for MHC Class II Molecules Associated with Alternative Ii Chain Isoforms
J. Immunol., April 1, 1998; 160(7): 3101 - 3110.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. Amigorena, D. Lankar, V. Briken, L. Gapin, M. Viguier, and C. Bonnerot
Type II and III Receptors for Immunoglobulin G (IgG) Control the Presentation of Different T Cell Epitopes from Single IgG-complexed Antigens
J. Exp. Med., February 16, 1998; 187(4): 505 - 515.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Lo-Man, J. P. M. Langeveld, P. Martineau, M. Hofnung, R. H. Meloen, and C. Leclerc
Immunodominance Does Not Result from Peptide Competition for MHC Class II Presentation
J. Immunol., February 15, 1998; 160(4): 1759 - 1766.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
S. Kovats, C. E. Grubin, S. Eastman, P. deRoos, A. Dongre, L. V. Kaer, and A. Y. Rudensky
Invariant Chain-independent Function of H-2M in the Formation of Endogenous Peptide-Major Histocompatibility Complex Class II Complexes In Vivo
J. Exp. Med., January 19, 1998; 187(2): 245 - 251.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N Barois, F Forquet, and J Davoust
Actin microfilaments control the MHC class II antigen presentation pathway in B cells
J. Cell Sci., January 7, 1998; 111(13): 1791 - 1800.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Horig, N. J. Papadopoulos, Z. Vegh, E. Palmieri, R. H. Angeletti, and S. G. Nathenson
An in vitro study of the dynamic features of the major histocompatibility complex class I complex relevant to its role as a versatile peptide-receptive molecule
PNAS, December 9, 1997; 94(25): 13826 - 13831.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. J. Kleijmeer, S. Morkowski, J. M. Griffith, A. Y. Rudensky, and H. J. Geuze
Major Histocompatibility Complex Class II Compartments in Human and Mouse B Lymphoblasts Represent Conventional Endocytic Compartments
J. Cell Biol., November 3, 1997; 139(3): 639 - 649.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1997 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1997 by The American Association of Immunologists, Inc. All rights reserved.