| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 158, Issue 3 1068-1077, Copyright © 1997 by American Association of Immunologists
ARTICLES |
CE Hornquist, X Lu, PM Rogers-Fani, U Rudolph, S Shappell, L Birnbaumer and GR Harriman
Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
Mice with targeted deletion of the G protein G(alpha)i2 develop an inflammatory bowel disease closely resembling ulcerative colitis. To better define disease pathogenesis, the mucosal immune system in G(alpha)i2-deficient mice was studied. Phenotypic analysis of large intestine lamina propria lymphocytes revealed a large increase in memory CD4+ T cells (CD44high, CD45RBlow, CD62Llow). Furthermore, expression of the mucosal homing receptor integrin beta7 was increased on mucosal, but not systemic, CD4+ T cells. Analysis of cytokine production revealed a marked increase in proinflammatory Th1-type cytokines in inflamed colons, as compared with wild-type mice or G(alpha)i2-deficient mice without colitis. Thus, IFN-gamma and IL-1beta levels were increased 13-fold and 30-fold, respectively, with more modest increases in IL-6 levels (5-fold) and TNF levels (2-fold). Inflamed colons of G(alpha)i2-deficient mice also demonstrated increased IL-12 p40 mRNA levels. No increase in IL-2, IL-4, IL-5, and IL-10 was seen. Large intestinal epithelial cells in G(alpha)i2- deficient mice with colitis were found by immunohistochemistry to express increased levels of both MHC class I and class II Ags. Colitis was associated with increased IgG levels (60-fold increase), predominantly IgG2a (135-fold increase), in large but not small intestinal secretions. This was shown by ELISPOT analysis to result from local production within the lamina propria.
This article has been cited by other articles:
|
|
 |
|
  B. Hoang, A. Trinh, and R. A. Edwards Decreased MAPK- and PGE2-dependent IL-11 production in Gi{alpha}2-/- colonic myofibroblasts Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1511 - G1519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. M. Padigel, L. Stein, K. Redding, J. J. Lee, T. J. Nolan, G. A. Schad, L. Birnbaumer, and D. Abraham Signaling through G{alpha}i2 protein is required for recruitment of neutrophils for antibody-mediated elimination of larval Strongyloides stercoralis in mice J. Leukoc. Biol., April 1, 2007; 81(4): 1120 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. D. Thompson, Y. Jin, K. H. Wu, R. A. Colvin, A. D. Luster, L. Birnbaumer, and M. X. Wu Inhibition of G{alpha}i2 Activation by G{alpha}i3 in CXCR3-mediated Signaling J. Biol. Chem., March 30, 2007; 282(13): 9547 - 9555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D W Hommes, T L Mikhajlova, S Stoinov, D Stimac, B Vucelic, J Lonovics, M Zakuciova, G D'Haens, G Van Assche, S Ba, et al. Fontolizumab, a humanised anti-interferon {gamma} antibody, demonstrates safety and clinical activity in patients with moderate to severe Crohn's disease Gut, August 1, 2006; 55(8): 1131 - 1137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Wettschureck and S. Offermanns Mammalian G Proteins and Their Cell Type Specific Functions Physiol Rev, October 1, 2005; 85(4): 1159 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fan, B. Zingarelli, O. M. Peck, G. Teti, G. E. Tempel, P. V. Halushka, and J. A. Cook Lipopolysaccharide- and gram-positive bacteria-induced cellular inflammatory responses: role of heterotrimeric G{alpha}i proteins Am J Physiol Cell Physiol, August 1, 2005; 289(2): C293 - C301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Wu, Y. Jin, R. A. Edwards, Y. Zhang, M. J. Finegold, and M. X. Wu Impaired TGF-{beta} Responses in Peripheral T Cells of G{alpha}i2-/- Mice J. Immunol., May 15, 2005; 174(10): 6122 - 6128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Habtezion, D. M. Toivola, E. C. Butcher, and M. B. Omary Keratin-8-deficient mice develop chronic spontaneous Th2 colitis amenable to antibiotic treatment J. Cell Sci., May 1, 2005; 118(9): 1971 - 1980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang, M. J. Finegold, Y. Jin, and M. X. Wu Accelerated transition from the double-positive to single-positive thymocytes in G{alpha}i2-deficient mice Int. Immunol., March 1, 2005; 17(3): 233 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Wei, P. Velazquez, O. Turovskaya, K. Spricher, R. Aranda, M. Kronenberg, L. Birnbaumer, and J. Braun Mesenteric B cells centrally inhibit CD4+ T cell colitis through interaction with regulatory T cell subsets PNAS, February 8, 2005; 102(6): 2010 - 2015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A M Westendorf, M Templin, R Geffers, S Deppenmeier, A D Gruber, M Probst-Kepper, W Hansen, R S Liblau, F Gunzer, D Bruder, et al. CD4+ T cell mediated intestinal immunity: chronic inflammation versus immune regulation Gut, January 1, 2005; 54(1): 60 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Takeshita, K. B. Bacon, and F. Gantner Critical Role of L-Selectin and Histamine H4 Receptor in Zymosan-Induced Neutrophil Recruitment from the Bone Marrow: Comparison with Carrageenan J. Pharmacol. Exp. Ther., July 1, 2004; 310(1): 272 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. T. Huang, Y. Zong, H. Dalwadi, C. Chung, M. C. Miceli, K. Spicher, L. Birnbaumer, J. Braun, and R. Aranda TCR-mediated hyper-responsiveness of autoimmune G{alpha}i2-/- mice is an intrinsic naive CD4+ T cell disorder selective for the G{alpha}i2 subunit Int. Immunol., November 1, 2003; 15(11): 1359 - 1367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. P. Singh, S. Singh, D. D. Taub, and J. W. Lillard Jr. Inhibition of IFN-{gamma}-Inducible Protein-10 Abrogates Colitis in IL-10-/- Mice J. Immunol., August 1, 2003; 171(3): 1401 - 1406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lippert, D. L. Yowe, J.-A. Gonzalo, J. P. Justice, J. M. Webster, E. R. Fedyk, M. Hodge, C. Miller, J.-C. Gutierrez-Ramos, F. Borrego, et al. Role of Regulator of G Protein Signaling 16 in Inflammation- Induced T Lymphocyte Migration and Activation J. Immunol., August 1, 2003; 171(3): 1542 - 1555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Dalwadi, B. Wei, M. Schrage, T. T. Su, D. J. Rawlings, and J. Braun B Cell Developmental Requirement for the G{alpha}i2 Gene J. Immunol., February 15, 2003; 170(4): 1707 - 1715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Ohman, L Franzen, U Rudolph, L Birnbaumer, and E H. Hornquist Regression of Peyer's patches in G{alpha}i2 deficient mice prior to colitis is associated with reduced expression of Bcl-2 and increased apoptosis Gut, September 1, 2002; 51(3): 392 - 397. [Abstract] [Full Text]
|
|
|
|
 |
|
 Genetically Modified Animals in Endocrinology Endocr. Rev., April 1, 2002; 23(2): 276 - 278. [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Braun, J. M. Wang, E. Lahey, R. L. Rabin, and B. L. Kelsall Activation of the formyl peptide receptor by the HIV-derived peptide T-20 suppresses interleukin-12 p70 production by human monocytes Blood, June 1, 2001; 97(11): 3531 - 3536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. He, S. Gurunathan, A. Iwasaki, B. Ash-Shaheed, and B. L. Kelsall Primary Role for Gi Protein Signaling in the Regulation of Interleukin 12 Production and the Induction of T Helper Cell Type 1 Responses J. Exp. Med., May 1, 2000; 191(9): 1605 - 1610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Braun, E. Lahey, and B. L. Kelsall Selective Suppression of IL-12 Production by Chemoattractants J. Immunol., March 15, 2000; 164(6): 3009 - 3017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wenzel-Seifert, J. M. Arthur, H.-Y. Liu, and R. Seifert Quantitative Analysis of Formyl Peptide Receptor Coupling to Gialpha 1, Gialpha 2, and Gialpha 3 J. Biol. Chem., November 19, 1999; 274(47): 33259 - 33266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Claesson, S. Bregenholt, K. Bonhagen, S. Thoma, P. Moller, M. J. Grusby, F. Leithauser, M. H. Nissen, and J. Reimann Colitis-Inducing Potency of CD4+ T Cells in Immunodeficient, Adoptive Hosts Depends on Their State of Activation, IL-12 Responsiveness, and CD45RB Surface Phenotype J. Immunol., March 15, 1999; 162(6): 3702 - 3710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Leach, N. J. Davidson, M. M. Fort, F. Powrie, and D. M. Rennick The Role of IL-10 in Inflammatory Bowel Disease: "Of Mice and Men" Toxicol Pathol, January 1, 1999; 27(1): 123 - 133. [Abstract] [PDF]
|
|
|
|
|
|
M. M. Fort, M. W. Leach, and D. M. Rennick A Role for NK Cells as Regulators of CD4+ T Cells in a Transfer Model of Colitis J. Immunol., October 1, 1998; 161(7): 3256 - 3261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Davidson, S. A. Hudak, R. E. Lesley, S. Menon, M. W. Leach, and D. M. Rennick IL-12, But Not IFN-{gamma}, Plays a Major Role in Sustaining the Chronic Phase of Colitis in IL-10-Deficient Mice J. Immunol., September 15, 1998; 161(6): 3143 - 3149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mahler, I. J. Bristol, E. H. Leiter, A. E. Workman, E. H. Birkenmeier, C. O. Elson, and J. P. Sundberg Differential susceptibility of inbred mouse strains to dextran sulfate sodium-induced colitis Am J Physiol Gastrointest Liver Physiol, March 1, 1998; 274(3): G544 - G551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yang, J. Wu, M. A. Kowalska, A. Dalvi, N. Prevost, P. J. O'Brien, D. Manning, M. Poncz, I. Lucki, J. A. Blendy, et al. Loss of signaling through the G protein, Gz, results in abnormal platelet activation and altered responses to psychoactive drugs PNAS, August 29, 2000; 97(18): 9984 - 9989. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
