| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 158, Issue 2 1014-1019, Copyright © 1997 by American Association of Immunologists
ARTICLES |
TW McCloskey, M Ott, E Tribble, SA Khan, S Teichberg, MO Paul, S Pahwa, E Verdin and N Chirmule
Department of Pediatrics, North Shore University Hospital-Cornell University Medical College, Manhasset, NY 11030, USA.
Apoptosis has been suggested to be one of the major mechanisms of depletion of CD4+ T cells in HIV-1-infected individuals. Remarkably, HIV-1-infected cells appear protected from apoptosis, whereas bystander cells show increased apoptosis in lymph nodes of infected individuals. In this work, we present evidence that the trans-activating protein of HIV-1, Tat, has a dual role in regulation of apoptosis in T cells. While addition of exogenous Tat protein induced apoptosis in uninfected T cells, T cell clones stably expressing the Tat protein were protected from activation-induced apoptosis. The addition of exogenous Tat potentiated anti-CD3 mAb, anti-Fas IgM mAb, and TNF-alpha-induced apoptosis of T cells. Pretreatment of Tat with anti-Tat Ab abrogated Tat-induced apoptosis, but did not affect anti-Fas IgM Ab-induced apoptosis. Endogenously expressed Tat was analyzed in Jurkat T cell clones transfected with either full-length tat gene (101 amino acids), or in control cells containing an empty vector. The Tat101-transfected clones were resistant to anti-CD3-induced apoptosis, when compared with cells transfected with vector alone. Furthermore, cross-linking of CD4 molecules on T cells with gp160 and anti-gp160 Ab showed markedly decreased apoptosis in Tat101 cells compared with that induced in cells transfected with vector alone. Taken together, our results indicate that HIV-1 Tat can regulate apoptosis that may contribute to the immunopathogenesis of AIDS.
This article has been cited by other articles:
|
|
 |
|
  T. Demberg, R. H. Florese, M. J. Heath, K. Larsen, I. Kalisz, V. S. Kalyanaraman, E. M. Lee, R. Pal, D. Venzon, R. Grant, et al. A Replication-Competent Adenovirus-Human Immunodeficiency Virus (Ad-HIV) tat and Ad-HIV env Priming/Tat and Envelope Protein Boosting Regimen Elicits Enhanced Protective Efficacy against Simian/Human Immunodeficiency Virus SHIV89.6P Challenge in Rhesus Macaques J. Virol., April 1, 2007; 81(7): 3414 - 3427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Madani, A. M. Hubicki, A. L. Perdigoto, M. Springer, and J. Sodroski Inhibition of Human Immunodeficiency Virus Envelope Glycoprotein- Mediated Single Cell Lysis by Low-Molecular-Weight Antagonists of Viral Entry J. Virol., January 15, 2007; 81(2): 532 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Campbell, J. D. Watkins, D. Esquieu, E. Pasquier, E. P. Loret, and S. A. Spector The C Terminus of HIV-1 Tat Modulates the Extent of CD178-mediated Apoptosis of T Cells J. Biol. Chem., November 18, 2005; 280(46): 38376 - 38382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Acheampong, Z. Parveen, L. W. Muthoga, V. Wasmuth-Peroud, M. Kalayeh, A. Bashir, R. Diecidue, M. Mukhtar, and R. J. Pomerantz Molecular Interactions of Human Immunodeficiency Virus Type 1 with Primary Human Oral Keratinocytes J. Virol., July 1, 2005; 79(13): 8440 - 8453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Campbell, E. Pasquier, J. Watkins, V. Bourgarel-Rey, V. Peyrot, D. Esquieu, P. Barbier, J. de Mareuil, D. Braguer, P. Kaleebu, et al. The Glutamine-rich Region of the HIV-1 Tat Protein Is Involved in T-cell Apoptosis J. Biol. Chem., November 12, 2004; 279(46): 48197 - 48204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-B. Huang, L. L. Jin, C. O. James, M. Khan, M. D. Powell, and V. C. Bond Characterization of Nef-CXCR4 Interactions Important for Apoptosis Induction J. Virol., October 15, 2004; 78(20): 11084 - 11096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Buttiglieri, M. C. Deregibus, S. Bravo, P. Cassoni, R. Chiarle, B. Bussolati, and G. Camussi Role of Pax2 in Apoptosis Resistance and Proinvasive Phenotype of Kaposi's Sarcoma Cells J. Biol. Chem., February 6, 2004; 279(6): 4136 - 4143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Komoto, S. Tsuji, M. S. Ibrahim, Y.-G. Li, J. Warachit, K. Taniguchi, and K. Ikuta The Vpu Protein of Human Immunodeficiency Virus Type 1 Plays a Protective Role against Virus-Induced Apoptosis in Primary CD4+ T Lymphocytes J. Virol., October 1, 2003; 77(19): 10304 - 10313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. LaBonte, N. Madani, and J. Sodroski Cytolysis by CCR5-Using Human Immunodeficiency Virus Type 1 Envelope Glycoproteins Is Dependent on Membrane Fusion and Can Be Inhibited by High Levels of CD4 Expression J. Virol., June 15, 2003; 77(12): 6645 - 6659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Varin, S. K. Manna, V. Quivy, A.-Z. Decrion, C. Van Lint, G. Herbein, and B. B. Aggarwal Exogenous Nef Protein Activates NF-kappa B, AP-1, and c-Jun N-Terminal Kinase and Stimulates HIV Transcription in Promonocytic Cells. ROLE IN AIDS PATHOGENESIS J. Biol. Chem., January 17, 2003; 278(4): 2219 - 2227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Twu, N. Q. Liu, W. Popik, M. Bukrinsky, J. Sayre, J. Roberts, S. Rania, V. Bramhandam, K. P. Roos, W. R. MacLellan, et al. Cardiomyocytes undergo apoptosis in human immunodeficiency virus cardiomyopathy through mitochondrion- and death receptor-controlled pathways PNAS, October 29, 2002; 99(22): 14386 - 14391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Agwale, M. T. Shata, M. S. Reitz Jr., V. S. Kalyanaraman, R. C. Gallo, M. Popovic, and D. M. Hone A Tat subunit vaccine confers protective immunity against the immune-modulating activity of the human immunodeficiency virus type-1 Tat protein in mice PNAS, July 23, 2002; 99(15): 10037 - 10041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Deregibus, V. Cantaluppi, S. Doublier, M. F. Brizzi, I. Deambrosis, A. Albini, and G. Camussi HIV-1-Tat Protein Activates Phosphatidylinositol 3-Kinase/ AKT-dependent Survival Pathways in Kaposi's Sarcoma Cells J. Biol. Chem., July 5, 2002; 277(28): 25195 - 25202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hay and G. Kannourakis A time to kill: viral manipulation of the cell death program J. Gen. Virol., June 1, 2002; 83(7): 1547 - 1564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cota-Gomez, N. C. Flores, C. Cruz, A. Casullo, T. Y. Aw, H. Ichikawa, J. Schaack, R. Scheinman, and S. C. Flores The Human Immunodeficiency Virus-1 Tat Protein Activates Human Umbilical Vein Endothelial Cell E-selectin Expression via an NF-kappa B-dependent Mechanism J. Biol. Chem., April 19, 2002; 277(17): 14390 - 14399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Lenardo, S. B. Angleman, V. Bounkeua, J. Dimas, M. G. Duvall, M. B. Graubard, F. Hornung, M. C. Selkirk, C. K. Speirs, C. Trageser, et al. Cytopathic Killing of Peripheral Blood CD4+ T Lymphocytes by Human Immunodeficiency Virus Type 1 Appears Necrotic rather than Apoptotic and Does Not Require env J. Virol., April 16, 2002; 76(10): 5082 - 5093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Bolton, B.-I. Hahn, E. A. Park, L. L. Lehnhoff, F. Hornung, and M. J. Lenardo Death of CD4+ T-Cell Lines Caused by Human Immunodeficiency Virus Type 1 Does Not Depend on Caspases or Apoptosis J. Virol., April 16, 2002; 76(10): 5094 - 5107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Gjoerup, D. Zaveri, and T. M. Roberts Induction of p53-Independent Apoptosis by Simian Virus 40 Small t Antigen J. Virol., October 1, 2001; 75(19): 9142 - 9155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chavan, S. Kodoth, R. Pahwa, and S. Pahwa The HIV protease inhibitor Indinavir inhibits cell-cycle progression in vitro in lymphocytes of HIV-infected and uninfected individuals Blood, July 15, 2001; 98(2): 383 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Etemad-Moghadam, D. Rhone, T. Steenbeke, Y. Sun, J. Manola, R. Gelman, J. W. Fanton, P. Racz, K. Tenner-Racz, M. K. Axthelm, et al. Membrane-Fusing Capacity of the Human Immunodeficiency Virus Envelope Proteins Determines the Efficiency of CD4+ T-Cell Depletion in Macaques Infected by a Simian-Human Immunodeficiency Virus J. Virol., June 15, 2001; 75(12): 5646 - 5655. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Gonzalez, C. Punzon, M. Gonzalez, and M. Fresno HIV-1 Tat Inhibits IL-2 Gene Transcription Through Qualitative and Quantitative Alterations of the Cooperative Rel/AP1 Complex Bound to the CD28RE/AP1 Composite Element of the IL-2 Promoter J. Immunol., April 1, 2001; 166(7): 4560 - 4569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Niehues, T. W. McCloskey, J. Ndagijimana, G. Horneff, V. Wahn, and S. Pahwa Apoptosis in T-Lymphocyte Subsets in Human Immunodeficiency Virus-Infected Children Measured Immediately Ex Vivo and following In Vitro Activation Clin. Vaccine Immunol., January 1, 2001; 8(1): 74 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Secchiero, D. Zella, S. Curreli, P. Mirandola, S. Capitani, R. C. Gallo, and G. Zauli Pivotal role of cyclic nucleoside phosphodiesterase 4 in Tat-mediated CD4+ T cell hyperactivation and HIV type 1 replication PNAS, December 8, 2000; (2000) 11512398. [Abstract] [Full Text]
|
|
|
|
|
|
U. Mahlknecht, C. Deng, M. C. Lu, T. C. Greenough, J. L. Sullivan, W. A. O'Brien, and G. Herbein Resistance to Apoptosis in HIV-Infected CD4+ T Lymphocytes Is Mediated by Macrophages: Role for Nef and Immune Activation in Viral Persistence J. Immunol., December 1, 2000; 165(11): 6437 - 6446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. LaBonte, T. Patel, W. Hofmann, and J. Sodroski Importance of Membrane Fusion Mediated by Human Immunodeficiency Virus Envelope Glycoproteins for Lysis of Primary CD4-Positive T Cells J. Virol., November 15, 2000; 74(22): 10690 - 10698. [Abstract] [Full Text]
|
|
|
|
|
|
S. K. Manna and B. B. Aggarwal Differential Requirement for p56lck in HIV-tat Versus TNF-Induced Cellular Responses: Effects on NF-{kappa}B, Activator Protein-1, c-Jun N-Terminal Kinase, and Apoptosis J. Immunol., May 15, 2000; 164(10): 5156 - 5166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, J. He, S. Fong, G. Wilcox, and C. Wood Jembrana Disease Virus Tat Can Regulate Human Immunodeficiency Virus (HIV) Long Terminal Repeat-Directed Gene Expression and Can Substitute for HIV Tat in Viral Replication J. Virol., March 15, 2000; 74(6): 2703 - 2713. [Abstract] [Full Text]
|
|
|
|
|
|
S. Mitola, R. Soldi, I. Zanon, L. Barra, M. I. Gutierrez, B. Berkhout, M. Giacca, and F. Bussolino Identification of Specific Molecular Structures of Human Immunodeficiency Virus Type 1 Tat Relevant for Its Biological Effects on Vascular Endothelial Cells J. Virol., January 1, 2000; 74(1): 344 - 353. [Abstract] [Full Text]
|
|
|
|
|
|
F. Kashanchi, E. T. Agbottah, C. A. Pise-Masison, R. Mahieux, J. Duvall, A. Kumar, and J. N. Brady Cell Cycle-Regulated Transcription by the Human Immunodeficiency Virus Type 1 Tat Transactivator J. Virol., January 1, 2000; 74(2): 652 - 660. [Abstract] [Full Text]
|
|
|
|
|
|
S. R. Bartz and M. Emerman Human Immunodeficiency Virus Type 1 Tat Induces Apoptosis and Increases Sensitivity to Apoptotic Signals by Up-Regulating FLICE/Caspase-8 J. Virol., March 1, 1999; 73(3): 1956 - 1963. [Abstract] [Full Text]
|
|
|
|
|
|
H. Chen, Y. K. Yip, I. George, M. Tyorkin, E. Salik, and K. Sperber Chronically HIV-1-Infected Monocytic Cells Induce Apoptosis in Cocultured T Cells J. Immunol., October 15, 1998; 161(8): 4257 - 4267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kaplan and S. Sieg Role of the Fas/Fas Ligand Apoptotic Pathway in Human Immunodeficiency Virus Type 1 Disease J. Virol., August 1, 1998; 72(8): 6279 - 6282. [Full Text] [PDF]
|
|
|
|
|
|
Z. Smit-McBride, J. J. Mattapallil, M. McChesney, D. Ferrick, and S. Dandekar Gastrointestinal T Lymphocytes Retain High Potential for Cytokine Responses but Have Severe CD4+ T-Cell Depletion at All Stages of Simian Immunodeficiency Virus Infection Compared to Peripheral Lymphocytes J. Virol., August 1, 1998; 72(8): 6646 - 6656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kumar, S. K. Manna, S. Dhawan, and B. B. Aggarwal HIV-Tat Protein Activates c-Jun N-Terminal Kinase and Activator Protein-1 J. Immunol., July 15, 1998; 161(2): 776 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gibellini, A. Bassini, S. Pierpaoli, L. Bertolaso, D. Milani, S. Capitani, M. La Placa, and G. Zauli Extracellular HIV-1 Tat Protein Induces the Rapid Ser133 Phosphorylation and Activation of CREB Transcription Factor in Both Jurkat Lymphoblastoid T Cells and Primary Peripheral Blood Mononuclear Cells J. Immunol., April 15, 1998; 160(8): 3891 - 3898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Conti, G. Rainaldi, P. Matarrese, B. Varano, R. Rivabene, S. Columba, A. Sato, F. Belardelli, W. Malorni, and S. Gessani The HIV-1 vpr Protein Acts as a Negative Regulator of Apoptosis in a Human Lymphoblastoid T Cell Line: Possible Implications for the Pathogenesis of AIDS J. Exp. Med., February 2, 1998; 187(3): 403 - 413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Herbein, C. Van Lint, J. L. Lovett, and E. Verdin Distinct Mechanisms Trigger Apoptosis in Human Immunodeficiency Virus Type 1-Infected and in Uninfected Bystander T Lymphocytes J. Virol., January 1, 1998; 72(1): 660 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Secchiero, D. Zella, S. Curreli, P. Mirandola, S. Capitani, R. C. Gallo, and G. Zauli Pivotal role of cyclic nucleoside phosphodiesterase 4 in Tat-mediated CD4+ T cell hyperactivation and HIV type 1 replication PNAS, December 19, 2000; 97(26): 14620 - 14625. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
