| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 158, Issue 12 5627-5631, Copyright © 1997 by American Association of Immunologists
ARTICLES |
H Groux, T Sornasse, F Cottrez, JE de Vries, RL Coffman, MG Roncarolo and H Yssel
Department of Human Immunology, DNAX Research Institute, Palo Alto, CA 94304, USA.
Differential expression of cytokine receptors accounts for an important regulatory mechanism in differentiation of Th1/Th2 subsets. Here, we report that human Th0 and Th2 clones constitutively express transcripts for the IFN-gammaR beta-chain, whereas mRNA for this signaling component of the IFN-gamma receptor is absent in Th1 clones. Activation of T cell clones, however, resulted in a transient induction or enhancement of IFN-gammaR beta-chain mRNA expression in Th1 clones and Th0/Th2 clones, respectively. IL-12-mediated Th1 cell differentiation of naive CD4+, CD45RA+ cord blood T cells, which constitutively express IFN-gammaR beta-chain mRNA, resulted in a loss of expression of this cytokine receptor chain after 6 to 12 days of culture. In contrast, Th2 populations, differentiated from CD4+, CD45RA+ cord blood T cells in the presence of IL-4, continued to express high levels of IFN-gammaR beta-chain transcripts. The loss of IFN-gammaR beta-chain expression in Th1 populations was accompanied by a failure of IFN-gamma to induce the expression of the IFN-gamma-inducible gene, IFN response factor-1, whereas IFN-gamma was effective in inducing IFN response factor-1 mRNA expression in Th0 and Th2 cells. These results indicate that down- regulation of the IFN-gammaR beta-chain correlates with impaired IFN- gamma-induced signaling in Th1 cells. Finally, Th2 populations, generated in the presence of both IL-4 and IFN-gamma, expressed levels of IFN-gammaR beta-chain transcripts similar to those produced by cells differentiated in the presence of IL-4 only, demonstrating that IFN- gamma does not modulate the expression of its receptor. Together, these data indicate that human Th0/Th2 and Th1 subsets, respectively, can be distinguished based on the expression of the IFN-gammaR beta-chain.
This article has been cited by other articles:
|
|
 |
|
  R. A. Clark, S. J. Huang, G. F. Murphy, I. G. Mollet, D. Hijnen, M. Muthukuru, C. F. Schanbacher, V. Edwards, D. M. Miller, J. E. Kim, et al. Human squamous cell carcinomas evade the immune response by down-regulation of vascular E-selectin and recruitment of regulatory T cells J. Exp. Med., September 29, 2008; 205(10): 2221 - 2234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Plaza, J. L. Rodriguez-Sanchez, and C. Juarez Staphylococcal Enterotoxin B In Vivo Modulates both Gamma Interferon Receptor Expression and Ligand-Induced Activation of Signal Transducer and Activator of Transcription 1 in T Cells Infect. Immun., January 1, 2007; 75(1): 306 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Clark, B. Chong, N. Mirchandani, N. K. Brinster, K.-i. Yamanaka, R. K. Dowgiert, and T. S. Kupper The Vast Majority of CLA+ T Cells Are Resident in Normal Skin J. Immunol., April 1, 2006; 176(7): 4431 - 4439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Cohen, E. Mouly, H. Hamdi, M.-C. Maillot, M. Pallardy, V. Godot, F. Capel, A. Balian, S. Naveau, P. Galanaud, et al. GILZ expression in human dendritic cells redirects their maturation and prevents antigen-specific T lymphocyte response Blood, March 1, 2006; 107(5): 2037 - 2044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Regis, M. Bosticardo, L. Conti, S. De Angelis, D. Boselli, B. Tomaino, P. Bernabei, M. Giovarelli, and F. Novelli Iron regulates T-lymphocyte sensitivity to the IFN-{gamma}/STAT1 signaling pathway in vitro and in vivo Blood, April 15, 2005; 105(8): 3214 - 3221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bernabei, M. Bosticardo, G. Losana, G. Regis, F. Di Paola, S. De Angelis, M. Giovarelli, and F. Novelli IGF-1 down-regulates IFN-{gamma}R2 chain surface expression and desensitizes IFN-{gamma}/STAT-1 signaling in human T lymphocytes Blood, October 15, 2003; 102(8): 2933 - 2939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Fukushima, K Fukata, A Ozaki, M Takata, N Kuroda, H Enzan, and H Ueno Exertion of the suppressive effects of IFN-{gamma} on experimental immune mediated blepharoconjunctivitis in Brown Norway rats during the induction phase but not the effector phase Br. J. Ophthalmol., October 1, 2002; 86(10): 1166 - 1171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Skrenta, Y. Yang, S. Pestka, and C. G. Fathman Ligand-Independent Down-Regulation of IFN-{gamma} Receptor 1 Following TCR Engagement J. Immunol., April 1, 2000; 164(7): 3506 - 3511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Rigamonti, S. Ariotti, G. Losana, R. Gradini, M. A. Russo, E. Jouanguy, J.-L. Casanova, G. Forni, and F. Novelli Surface Expression of the IFN-{gamma}R2 Chain Is Regulated by Intracellular Trafficking in Human T Lymphocytes J. Immunol., January 1, 2000; 164(1): 201 - 207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Allione, P. Bernabei, M. Bosticardo, S. Ariotti, G. Forni, and F. Novelli Nitric Oxide Suppresses Human T Lymphocyte Proliferation Through IFN-{gamma}-Dependent and IFN-{gamma}-Independent Induction of Apoptosis J. Immunol., October 15, 1999; 163(8): 4182 - 4191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Coccia, N. Passini, A. Battistini, C. Pini, F. Sinigaglia, and L. Rogge Interleukin-12 Induces Expression of Interferon Regulatory Factor-1 via Signal Transducer and Activator of Transcription-4 in Human T Helper Type 1 Cells J. Biol. Chem., March 5, 1999; 274(10): 6698 - 6703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Allione, V. Wells, G. Forni, L. Mallucci, and F. Novelli {beta}-Galactoside-Binding Protein ({beta}GBP) Alters the Cell Cycle, Up-Regulates Expression of the {alpha}- and {beta}-Chains of the IFN-{gamma} Receptor, and Triggers IFN-{gamma}-Mediated Apoptosis of Activated Human T Lymphocytes J. Immunol., September 1, 1998; 161(5): 2114 - 2119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Lu, C. Ebensperger, Z. Dembic, Y. Wang, M. Kvatyuk, T. Lu, R. L. Coffman, S. Pestka, and P. B. Rothman Targeted disruption of the interferon-gamma receptor 2 gene results in severe immune defects in mice PNAS, July 7, 1998; 95(14): 8233 - 8238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Amasaki, E. S. Masuda, R. Imamura, K.-i. Arai, and N. Arai Distinct NFAT Family Proteins Are Involved in the Nuclear NFAT-DNA Binding Complexes from Human Thymocyte Subsets J. Immunol., March 1, 1998; 160(5): 2324 - 2333. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
