The Journal of Immunology, Vol 158, Issue 11 5071-5074, Copyright © 1997 by American Association of Immunologists

CUTTING EDGE

Regulation of the RAG-1 promoter by the NF-Y transcription factor

ST Brown, GA Miranda, Z Galic, IZ Hartman, CJ Lyon and RJ Aguilera
Department of Molecular, Cell, and Developmental Biology, Molecular Biology Institute, University of California at Los Angeles, 90095, USA.

We have cloned the RAG-1 promoter region and have determined that almost all detectable promoter activity resides within a 208-bp fragment. Sequence analysis of this promoter region has identified potential recognition motifs for a number of lymphocyte-restricted and ubiquitous transcription factors. Subsequent assays have revealed that the NF-Y transcription factor interacts with a CCAAT site within the RAG-1 promoter and appears to play an important role in the positive transcriptional regulation of this gene.

This article has been cited by other articles:

				Z.-X. Jin, H. Kishi, X.-C. Wei, T. Matsuda, S. Saito, and A. Muraguchi Lymphoid Enhancer-Binding Factor-1 Binds and Activates the Recombination-Activating Gene-2 Promoter Together with c-Myb and Pax-5 in Immature B Cells J. Immunol., October 1, 2002; 169(7): 3783 - 3792. [Abstract] [Full Text] [PDF]

				X.-C. Wei, H. Kishi, Z.-X. Jin, W.-P. Zhao, S. Kondo, T. Matsuda, S. Saito, and A. Muraguchi Characterization of Chromatin Structure and Enhancer Elements for Murine Recombination Activating Gene-2 J. Immunol., July 15, 2002; 169(2): 873 - 881. [Abstract] [Full Text] [PDF]

				H. Kishi, Z.-X. Jin, X.-C. Wei, T. Nagata, T. Matsuda, S. Saito, and A. Muraguchi Cooperative binding of c-Myb and Pax-5 activates the RAG-2 promoter in immature B cells Blood, January 15, 2002; 99(2): 576 - 583. [Abstract] [Full Text] [PDF]

				N. Yannoutsos, P. Wilson, W. Yu, H. T. Chen, A. Nussenzweig, H. Petrie, and M. C. Nussenzweig The Role of Recombination Activating Gene (RAG) Reinduction in Thymocyte Development In Vivo J. Exp. Med., August 20, 2001; 194(4): 471 - 480. [Abstract] [Full Text] [PDF]

				H. Kishi, X.-C. Wei, Z.-X. Jin, Y. Fujishiro, T. Nagata, T. Matsuda, and A. Muraguchi Lineage-specific regulation of the murine RAG-2 promoter: GATA-3 in T cells and Pax-5 in B cells Blood, June 15, 2000; 95(12): 3845 - 3852. [Abstract] [Full Text] [PDF]

				R. J. Monroe, F. Chen, R. Ferrini, L. Davidson, and F. W. Alt RAG2 is regulated differentially in B and T cells by elements 5' of the promoter PNAS, October 26, 1999; 96(22): 12713 - 12718. [Abstract] [Full Text] [PDF]

				W. Yu, Z. Misulovin, H. Suh, R. R. Hardy, M. Jankovic, N. Yannoutsos, and M. C. Nussenzweig Coordinate Regulation of RAG1 and RAG2 by Cell Type-Specific DNA Elements 5' of RAG2 Science, August 13, 1999; 285(5430): 1080 - 1084. [Abstract] [Full Text]

				J. Lauring and M. S. Schlissel Distinct Factors Regulate the Murine RAG-2 Promoter in B- and T-Cell Lines Mol. Cell. Biol., April 1, 1999; 19(4): 2601 - 2612. [Abstract] [Full Text] [PDF]

				L. J. Kienker, M. R. Ghosh, and P. W. Tucker Regulatory Elements in the Promoter of a Murine TCRD V Gene Segment J. Immunol., July 15, 1998; 161(2): 791 - 804. [Abstract] [Full Text] [PDF]