| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 157, Issue 8 3617-3626, Copyright © 1996 by American Association of Immunologists
ARTICLES |
KM Skubitz, KD Campbell, J Iida and AP Skubitz
Department of Medicine, University of Minnesota Medical School, Minneapolis 55455, USA.
As a member of the tetraspan family, it has been hypothesized that CD63 may be associated with signal transduction; however, its role in leukocyte function is unknown. To examine the potential ability of CD63 to activate neutrophils, the effects of five CD63 mAbs, AHN-16, -16.1, - 16.2, -16.3, and -16.5, were examined for their ability to alter neutrophil adhesion to HUVEC monolayers. These CD63 Abs increased neutrophil adhesion to resting and TNF-stimulated HUVEC monolayers. This increase in neutrophil adhesion caused by CD63 Abs was blocked by a CD18 Ab and was associated with up-regulation of CD11/CD18 and down- regulation of CD62L on the neutrophil surface. CD11/CD18 was also found to be associated with CD63. This increase in neutrophil adhesion required physiologic extracellular calcium concentrations at or near the time of CD63 Ab binding. The incubation of CD63 Abs with cells in the absence of calcium for 10 min before repletion of calcium resulted in no increase in neutrophil adhesion. Protein kinase activity was detected in neutrophils associated with CD63. Most of the protein kinase activity associated with these Ags was tyrosine kinase activity, with a lesser amount of threonine and serine kinase activities. Src family kinases Lyn and Hck accounted for much of the associated tyrosine kinase activity. The data suggest that CD63 Ab binding to the neutrophil surface triggers a transient activation signal that requires extracellular calcium and regulates the adhesive activity of CD11/CD18. Associated protein kinase activity may play a role in signal transduction by CD63 to regulate other cell functions.
This article has been cited by other articles:
|
|
 |
|
  D. Lin, E.-J. Kamsteeg, Y. Zhang, Y. Jin, H. Sterling, P. Yue, M. Roos, A. Duffield, J. Spencer, M. Caplan, et al. Expression of Tetraspan Protein CD63 Activates Protein-tyrosine Kinase (PTK) and Enhances the PTK-induced Inhibition of ROMK Channels J. Biol. Chem., March 21, 2008; 283(12): 7674 - 7681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Chen, J. Tang, and P. Stanley Suppressors of {alpha}(1,3)fucosylation identified by expression cloning in the LEC11B gain-of-function CHO mutant Glycobiology, March 1, 2005; 15(3): 259 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Mantegazza, M. M. Barrio, S. Moutel, L. Bover, M. Weck, P. Brossart, J.-L. Teillaud, and J. Mordoh CD63 tetraspanin slows down cell migration and translocates to the endosomal-lysosomal-MIICs route after extracellular stimuli in human immature dendritic cells Blood, August 15, 2004; 104(4): 1183 - 1190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Rarok, P. C. Limburg, and C. G. M. Kallenberg Neutrophil-activating potential of antineutrophil cytoplasm autoantibodies J. Leukoc. Biol., July 1, 2003; 74(1): 3 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. von Lindern, D. Rojo, K. Grovit-Ferbas, C. Yeramian, C. Deng, G. Herbein, M. R. Ferguson, T. C. Pappas, J. M. Decker, A. Singh, et al. Potential Role for CD63 in CCR5-Mediated Human Immunodeficiency Virus Type 1 Infection of Macrophages J. Virol., March 15, 2003; 77(6): 3624 - 3633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Roberts, A. J. Woods, P. E. Shaw, and J. C. Norman ERK1 Associates with alpha vbeta 3 Integrin and Regulates Cell Spreading on Vitronectin J. Biol. Chem., January 10, 2003; 278(3): 1975 - 1985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Fazal, W. M. Al-Ghoul, M. J. Schmidt, M. A. Choudhry, and M. M. Sayeed Lyn- and ERK-mediated vs. Ca2+-mediated neutrophil O2- responses with thermal injury Am J Physiol Cell Physiol, November 1, 2002; 283(5): C1469 - C1479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-G. Castaigne, W. Guo, C. Leveille, D. Charron, and R. Al-Daccak A CD18-dependent protein kinase C {beta}-mediated alternative cell death pathway of activated monocytes Int. Immunol., September 1, 2002; 14(9): 1003 - 1014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Anzai, Y. Lee, B.-S. Youn, S. Fukuda, Y.-J. Kim, C. Mantel, M. Akashi, and H. E. Broxmeyer c-kit associated with the transmembrane 4 superfamily proteins constitutes a functionally distinct subunit in human hematopoietic progenitors Blood, May 29, 2002; 99(12): 4413 - 4421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mahmudi-Azer, G. P. Downey, and R. Moqbel Translocation of the tetraspanin CD63 in association with human eosinophil mediator release Blood, May 13, 2002; 99(11): 4039 - 4047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Berditchevski Complexes of tetraspanins with integrins: more than meets the eye J. Cell Sci., January 12, 2001; 114(23): 4143 - 4151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Skubitz, K. D. Campbell, and A. P. N. Skubitz Synthetic Peptides of CD66a Stimulate Neutrophil Adhesion to Endothelial Cells J. Immunol., April 15, 2000; 164(8): 4257 - 4264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Stipp and M. Hemler Transmembrane-4-superfamily proteins CD151 and CD81 associate with alpha 3 beta 1 integrin, and selectively contribute to alpha 3 beta 1-dependent neurite outgrowth J. Cell Sci., January 6, 2000; 113(11): 1871 - 1882. [Abstract] [PDF]
|
|
|
|
|
|
H. F.G. Heijnen, A. E. Schiel, R. Fijnheer, H. J. Geuze, and J. J. Sixma Activated Platelets Release Two Types of Membrane Vesicles: Microvesicles by Surface Shedding and Exosomes Derived From Exocytosis of Multivesicular Bodies and alpha -Granules Blood, December 1, 1999; 94(11): 3791 - 3799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Karp, M. L. Carlisle, A. B. Mobley, T. C. Nichols, N. Oppenheimer-Marks, R. I. Brezinschek, and V. M. Holers {gamma}-Glutamyl transpeptidase is up-regulated on memory T lymphocytes Int. Immunol., November 1, 1999; 11(11): 1791 - 1800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Berditchevski and E. Odintsova Characterization of Integrin–Tetraspanin Adhesion Complexes: Role of Tetraspanins in Integrin Signaling J. Cell Biol., July 26, 1999; 146(2): 477 - 492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Okada, M. Sanezumi, T. Nakajima, S. Okada, K. Yasuda, and H. Kanzaki Rapid down-regulation of CD63 transcription by progesterone in human endometrial stromal cells Mol. Hum. Reprod., June 1, 1999; 5(6): 554 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sincock, S Fitter, R. Parton, M. Berndt, J. Gamble, and L. Ashman PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function J. Cell Sci., January 3, 1999; 112(6): 833 - 844. [Abstract] [PDF]
|
|
|
|
 |
|
 A. E. May, S. M. Kanse, L. R. Lund, R. H. Gisler, B. A. Imhof, and K. T. Preissner Urokinase Receptor (CD87) Regulates Leukocyte Recruitment via beta 2 Integrins In Vivo J. Exp. Med., September 21, 1998; 188(6): 1029 - 1037. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Avni, Z. Pur, E. Yefenof, and M. Baniyash Complement Receptor 3 of Macrophages Is Associated with Galectin-1-Like Protein J. Immunol., June 15, 1998; 160(12): 6151 - 6158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yanez-Mo, A. Alfranca, C. Cabanas, M. Marazuela, R. Tejedor, M. Angeles Ursa, L. K. Ashman, M. O. de Landazuri, and F. Sanchez-Madrid Regulation of Endothelial Cell Motility by Complexes of Tetraspan Molecules CD81/TAPA-1 and CD151/PETA-3 with alpha 3beta 1 Integrin Localized at Endothelial Lateral Junctions J. Cell Biol., May 4, 1998; 141(3): 791 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Tachibana, J. Bodorova, F. Berditchevski, M. M. Zutter, and M. E. Hemler NAG-2, a Novel Transmembrane-4 Superfamily (TM4SF) Protein That Complexes with Integrins and Other TM4SF Proteins J. Biol. Chem., November 14, 1997; 272(46): 29181 - 29189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Claas, C. S. Stipp, and M. E. Hemler Evaluation of Prototype Transmembrane 4 Superfamily Protein Complexes and Their Relation to Lipid Rafts J. Biol. Chem., March 9, 2001; 276(11): 7974 - 7984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sixt, R. Hallmann, O. Wendler, K. Scharffetter-Kochanek, and L. M. Sorokin Cell Adhesion and Migration Properties of beta 2-Integrin Negative Polymorphonuclear Granulocytes on Defined Extracellular Matrix Molecules. RELEVANCE FOR LEUKOCYTE EXTRAVASATION J. Biol. Chem., May 25, 2001; 276(22): 18878 - 18887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Stipp, T. V. Kolesnikova, and M. E. Hemler EWI-2 Is a Major CD9 and CD81 Partner and Member of a Novel Ig Protein Subfamily J. Biol. Chem., October 26, 2001; 276(44): 40545 - 40554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Woods and J. R. Couchman Integrin Modulation by Lateral Association J. Biol. Chem., August 4, 2000; 275(32): 24233 - 24236. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
