The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goth, S.
Right arrow Articles by Shastri, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goth, S.
Right arrow Articles by Shastri, N.

The Journal of Immunology, Vol 157, Issue 5 1894-1904, Copyright © 1996 by American Association of Immunologists

ARTICLES

Generation of naturally processed peptide/MHC class I complexes is independent of the stability of endogenously synthesized precursors

S Goth, V Nguyen and N Shastri
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.

Proteolysis of endogenously synthesized cellular proteins is essential for constitutive display of processed peptide/MHC class I complexes on the APC surface for stimulating CD8+ T cells. However, the extent to which normal protein turnover serves as the source of processed peptides is not clear. To address this question, we used pairs of novel N-end rule substrates that varied in their intracellular stability and served as precursors for generating peptide/MHC class I (OVA257-264/Kb or influenza nucleoprotein 366-374/Db) complexes. Surprisingly, although each of three precursor pairs tested varied profoundly in their intracellular stability, they were indistinguishable in either T cell stimulation assays, or in the amounts of naturally processed peptides in the APC extracts. Our findings demonstrate that the proteolytic turnover of endogenously synthesized proteins is not directly proportional to the generation of processed antigenic peptide/MHC class I complexes.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. Goldwich, S. S. C. Hahn, S. Schreiber, S. Meier, E. Kampgen, R. Wagner, M. B. Lutz, and U. Schubert
Targeting HIV-1 Gag into the Defective Ribosomal Product Pathway Enhances MHC Class I Antigen Presentation and CD8+ T Cell Activation
J. Immunol., January 1, 2008; 180(1): 372 - 382.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Schirmbeck, P. Riedl, M. Kupferschmitt, U. Wegenka, H. Hauser, J. Rice, A. Kroger, and J. Reimann
Priming Protective CD8 T Cell Immunity by DNA Vaccines Encoding Chimeric, Stress Protein-Capturing Tumor-Associated Antigen
J. Immunol., August 1, 2006; 177(3): 1534 - 1542.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Ostankovitch, V. Robila, and V. H. Engelhard
Regulated Folding of Tyrosinase in the Endoplasmic Reticulum Demonstrates That Misfolded Full-Length Proteins Are Efficient Substrates for Class I Processing and Presentation
J. Immunol., March 1, 2005; 174(5): 2544 - 2551.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. B. J. Wong, C. B. Buck, X. Shen, and R. F. Siliciano
An Evaluation of Enforced Rapid Proteasomal Degradation as a Means of Enhancing Vaccine-Induced CTL Responses
J. Immunol., September 1, 2004; 173(5): 3073 - 3083.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. N. Golovina, E. J. Wherry, T. N. J. Bullock, and L. C. Eisenlohr
Efficient and Qualitatively Distinct MHC Class I-Restricted Presentation of Antigen Targeted to the Endoplasmic Reticulum
J. Immunol., March 15, 2002; 168(6): 2667 - 2675.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Schwarz, R. de Giuli, G. Schmidtke, S. Kostka, M. van den Broek, K. B. Kim, C. M. Crews, R. Kraft, and M. Groettrup
The Selective Proteasome Inhibitors Lactacystin and Epoxomicin Can Be Used to Either Up- or Down-Regulate Antigen Presentation at Nontoxic Doses
J. Immunol., June 15, 2000; 164(12): 6147 - 6157.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Tobery and R. F. Siliciano
Cutting Edge: Induction of Enhanced CTL-Dependent Protective Immunity In Vivo by N-End Rule Targeting of a Model Tumor Antigen
J. Immunol., January 15, 1999; 162(2): 639 - 642.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. C. Anton, H. L. Snyder, J. R. Bennink, A. Vinitsky, M. Orlowski, A. Porgador, and J. W. Yewdell
Dissociation of Proteasomal Degradation of Biosynthesized Viral Proteins from Generation of MHC Class I-Associated Antigenic Peptides
J. Immunol., May 15, 1998; 160(10): 4859 - 4868.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. A. M. Sijts, I. Pilip, and E. G. Pamer
The Listeria monocytogenes-secreted p60 Protein Is an N-end Rule Substrate in the Cytosol of Infected Cells. IMPLICATIONS FOR MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I ANTIGEN PROCESSING OF BACTERIAL PROTEINS
J. Biol. Chem., August 1, 1997; 272(31): 19261 - 19268.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
T. W. Tobery and R. F. Siliciano
Targeting of HIV-1 Antigens for Rapid Intracellular Degradation Enhances Cytotoxic T Lymphocyte (CTL) Recognition and the Induction of De Novo CTL Responses In Vivo After Immunization
J. Exp. Med., March 3, 1997; 185(5): 909 - 920.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.