| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 157, Issue 4 1576-1581, Copyright © 1996 by American Association of Immunologists
ARTICLES |
A Tamm and RE Schmidt
Department of Clinical Immunology, Hannover Medical School, Germany.
Numerous mAbs have been generated against Fc gamma RIII (CD16), the low- affinity receptor for the Fc part of IgG. Most of the mAbs recognize both the receptor isoforms, transmembranous Fc gamma RIIIA, and glycosylphosphatidylinositol-linked Fc gamma RIIIB. Binding epitopes of some of the mAbs that differentiate between the two neutrophil Ag (NA) alleles of Fc gamma RIIIB (CLB-Gran11 against NA1; GRM1, BL-LGL/1 against NA2 allele) have been mapped on the first, membrane-distal domain of CD16. We demonstrate that mAbs 3G8, B88-9, CLB-Gran1, MEM- 154, and LNK16 almost completely block the receptor's interaction with IgG. Using chimeric Fc gamma RIIIB/Fc epsilon RI receptors and molecular modeling, we localized the epitopes of 3G8 and B88-9 on the putative FG loop of the membrane-proximal Ig-like domain, which we have previously identified as the major binding site for IgG. The epitopes of CLB-Gran1 and MEM-154 are shown to reside in proximity to the FG loop (probably BC or C'E loop). The blocking mAb LNK16 was detected to be directed against the putative C' beta-sheet of the membrane-proximal domain, suggesting that additional residues may be involved in IgG binding of Fc gamma RIII.
This article has been cited by other articles:
|
|
 |
|
  N. Congy-Jolivet, A. Bolzec, D. Ternant, M. Ohresser, H. Watier, and G. Thibault Fc{gamma}RIIIa Expression Is Not Increased on Natural Killer Cells Expressing the Fc{gamma}RIIIa-158V Allotype Cancer Res., February 15, 2008; 68(4): 976 - 980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Li, N. Jiang, S. Nagarajan, R. Wohlhueter, P. Selvaraj, and C. Zhu Affinity and Kinetic Analysis of Fc{gamma} Receptor IIIa (CD16a) Binding to IgG Ligands J. Biol. Chem., March 2, 2007; 282(9): 6210 - 6221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Wei, J. W. Stallworth, P. J. Vance, J. A. Hoxie, and P. N. Fultz Simian Immunodeficiency Virus (SIV)/Immunoglobulin G Immune Complexes in SIV-Infected Macaques Block Detection of CD16 but Not Cytolytic Activity of Natural Killer Cells. Clin. Vaccine Immunol., July 1, 2006; 13(7): 768 - 778. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Mende, P. Hoffmann, A. Wolf, R. Lutterbuse, E. Kopp, P. A. Baeuerle, A. de Baey, and P. Kufer Highly efficient antigen targeting to M-DC8+ dendritic cells via Fc{gamma}RIII/CD16-specific antibody conjugates Int. Immunol., May 1, 2005; 17(5): 539 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Oshima, A. Fujimura, G. Cartron, L. Dacheux, G. Salles, P. Solal-Celigny, P. Bardos, P. Colombat, and H. Watier Implication for how the single nucleotide polymorphism (SNP) of Fc receptor, Fcgamma RIIIa alters the interaction with anti-CD20 monoclonal antibody Blood, May 29, 2002; 99(12): 4649 - 4650. [Full Text] [PDF]
|
|
|
|
|
|
S. E. Chesla, P. Li, S. Nagarajan, P. Selvaraj, and C. Zhu The Membrane Anchor Influences Ligand Binding Two-dimensional Kinetic Rates and Three-dimensional Affinity of Fcgamma RIII (CD16) J. Biol. Chem., March 31, 2000; 275(14): 10235 - 10246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Craig Morton, G. van Zandbergen, C. van Kooten, C. J. Howard, J. G. J. van de Winkel, and P. Brandtzaeg Immunoglobulin-binding Sites of Human Fcalpha RI (CD89) and Bovine Fcgamma 2R Are Located in their Membrane-distal Extracellular Domains J. Exp. Med., June 7, 1999; 189(11): 1715 - 1722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhu, K. J. Hamann, N. M. Munoz, N. Rubio, D. Mayer, A. Hernrreiter, and A. R. Leff Intracellular Expression of Fc{gamma}RIII (CD16) and Its Mobilization by Chemoattractants in Human Eosinophils J. Immunol., September 1, 1998; 161(5): 2574 - 2579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. R. Koene, M. Kleijer, J. Algra, D. Roos, A. E.G.Kr. von dem Borne, and M. de Haas Fcgamma RIIIa-158V/F Polymorphism Influences the Binding of IgG by Natural Killer Cell Fcgamma RIIIa, Independently of the Fcgamma RIIIa-48L/R/H Phenotype Blood, August 1, 1997; 90(3): 1109 - 1114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Williams, S. Nagarajan, P. Selvaraj, and C. Zhu Quantifying the Impact of Membrane Microtopology on Effective Two-dimensional Affinity J. Biol. Chem., April 13, 2001; 276(16): 13283 - 13288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Vandorpe, S. Wilhelm, L. Jiang, O. Ibraghimov-Beskrovnaya, M. N. Chernova, A. K. Stuart-Tilley, and S. L. Alper Cation channel regulation by COOH-terminal cytoplasmic tail of polycystin-1: mutational and functional analysis Physiol Genomics, February 28, 2002; 8(2): 87 - 98. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
