| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The Journal of Immunology, Vol 157, Issue 12 5277-5283, Copyright © 1996 by American Association of Immunologists
ARTICLES |
T Kanno and U Siebenlist
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Signals transduced via the TCR activate the transcription factor nuclear factor-kappaB (NF-kappaB), which, in turn, is critical to the transcriptional induction of many genes important for the proliferation and expression of a differentiated phenotype. Treatment of T cells with the protein kinase C activator PMA in combination with Ca2+ ionophores mimics this process, and the two agents are often substituted for TCR stimulation, bypassing the TCR. Here we identify intracellular signaling components involved in activation of NF-kappaB following TCR stimulation. TCR signaling was triggered by treating Jurkat T cells with PHA or anti-CD3 Abs, and NF-kappaB activation was monitored by electrophoretic mobility shift assays and/or by kappaB-dependent reporter assays. Contrary to the idea that protein kinase C is involved in TCR-mediated activation of NF-kappaB, high doses of staurosporine did not interfere with activation of NF-kappaB by PHA, while the same dose of staurosporine completely blocked activation by PMA. PHA-induced kappaB-dependent reporter activity was, however, effectively blocked by a dominant negative form of Raf-1, suggesting a critical role for a Raf kinase. The TCR-mediated activation of NF-kappaB was also dependent on a Ca2+ influx, because the Ca2+ channel blocker, SK&F 96365, as well as other agents that prevented the Ca2+ influx, inhibited NF-kappaB activation. Cotransfection of a constitutively active form of calcineurin largely substituted for the Ca2+ requirement and reversed the blockade by SK&F 96365. Consistent with these observations, coexpression of constitutively active forms of Raf-1 and calcineurin synergistically induced kappaB-dependent reporter activity, suggesting a physiologically relevant functional interaction between the kinase and the phosphatase.
This article has been cited by other articles:
|
|
 |
|
  Y. S. Kim, K.-O. Cho, H. J. Lee, S. Y. Kim, Y. Sato, and Y.-J. Cho Down Syndrome Candidate Region 1 Increases the Stability of the I{kappa}B{alpha} Protein: IMPLICATIONS FOR ITS ANTI-INFLAMMATORY EFFECTS J. Biol. Chem., December 22, 2006; 281(51): 39051 - 39061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Chiodetti, S. Choi, D. L. Barber, and R. H. Schwartz Adaptive Tolerance and Clonal Anergy Are Distinct Biochemical States J. Immunol., February 15, 2006; 176(4): 2279 - 2291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Trushin, K. N. Pennington, E. M. Carmona, S. Asin, D. N. Savoy, D. D. Billadeau, and C. V. Paya Protein Kinase C{alpha} (PKC{alpha}) Acts Upstream of PKC{theta} To Activate I{kappa}B Kinase and NF-{kappa}B in T Lymphocytes Mol. Cell. Biol., October 1, 2003; 23(19): 7068 - 7081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Bernal-Mizrachi, W. Wen, M. Shornick, and M. A. Permutt Activation of Nuclear Factor-{kappa}B by Depolarization and Ca2+ Influx in MIN6 Insulinoma Cells Diabetes, December 1, 2002; 51(90003): S484 - 488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Ding, B. Albrecht, R. E. Kelley, N. Muthusamy, S.-J. Kim, R. A. Altschuld, and M. D. Lairmore Human T-Cell Lymphotropic Virus Type 1 p12I Expression Increases Cytoplasmic Calcium To Enhance the Activation of Nuclear Factor of Activated T Cells J. Virol., September 11, 2002; 76(20): 10374 - 10382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. I. Gringhuis, E. A. M. Papendrecht-van der Voort, A. Leow, E. W. N. Levarht, F. C. Breedveld, and C. L. Verweij Effect of Redox Balance Alterations on Cellular Localization of LAT and Downstream T-Cell Receptor Signaling Pathways Mol. Cell. Biol., January 15, 2002; 22(2): 400 - 411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Herndon, X. C. Shan, G. C. Tsokos, and R. L. Wange ZAP-70 and SLP-76 Regulate Protein Kinase C-{{theta}} and NF-{{kappa}}B Activation in Response to Engagement of CD3 and CD28 J. Immunol., May 1, 2001; 166(9): 5654 - 5664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Chakravortty, Y. Kato, T. Sugiyama, N. Koide, M. M. Mu, T. Yoshida, and T. Yokochi The Inhibitory Action of Sodium Arsenite on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 267.4 Macrophage Cells: A Role of Raf-1 in Lipopolysaccharide Signaling J. Immunol., February 1, 2001; 166(3): 2011 - 2017. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Amrani, A. L. Lazaar, R. Hoffman, K. Amin, S. Ousmer, and R. A. Panettieri Jr. Activation of p55 Tumor Necrosis Factor-alpha Receptor-1 Coupled to Tumor Necrosis Factor Receptor-Associated Factor 2 Stimulates Intercellular Adhesion Molecule-1 Expression by Modulating a Thapsigargin-Sensitive Pathway in Human Tracheal Smooth Muscle Cells Mol. Pharmacol., July 1, 2000; 58(1): 237 - 245. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. D. Goldman, A. L. Gilman, C. Hollenback, R. M. Kato, B. A. Premack, and D. J. Rawlings Hydroxychloroquine inhibits calcium signals in T cells: a new mechanism to explain its immunomodulatory properties Blood, June 1, 2000; 95(11): 3460 - 3466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Cissel and M. A. Beaven Disruption of Raf-1/Heat Shock Protein 90 Complex and Raf Signaling by Dexamethasone in Mast Cells J. Biol. Chem., March 15, 2000; 275(10): 7066 - 7070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ouellet, B. Barbeau, and M. J. Tremblay p56lck, ZAP-70, SLP-76, and Calcium-regulated Effectors Are Involved in NF-kappa B Activation by Bisperoxovanadium Phosphotyrosyl Phosphatase Inhibitors in Human T Cells J. Biol. Chem., December 3, 1999; 274(49): 35029 - 35036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Hu, S. Deshpande, K. Irani, and R. C. Ziegelstein [Ca2+]i Oscillation Frequency Regulates Agonist-stimulated NF-kappa B Transcriptional Activity J. Biol. Chem., November 26, 1999; 274(48): 33995 - 33998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. ALEXANIAN and J. R. BAMBURG Neuronal survival activity of S100{beta}{beta} is enhanced by calcineurin inhibitors and requires activation of NF-{kappa}B FASEB J, September 1, 1999; 13(12): 1611 - 1620. [Abstract] [Full Text]
|
|
|
|
|
|
M. Hinz, D. Krappmann, A. Eichten, A. Heder, C. Scheidereit, and M. Strauss NF-kappa B Function in Growth Control: Regulation of Cyclin D1 Expression and G0/G1-to-S-Phase Transition Mol. Cell. Biol., April 1, 1999; 19(4): 2690 - 2698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. Costello, A. E. Walters, P. J. Mee, M. Turner, L. F. Reynolds, A. Prisco, N. Sarner, R. Zamoyska, and V. L. J. Tybulewicz The Rho-family GTP exchange factor Vav is a critical transducer of T cell receptor signals to the calcium, ERK, and NF-kappa B pathways PNAS, March 16, 1999; 96(6): 3035 - 3040. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Schwiebert, K. Estell, and S. M. Propst Chemokine expression in CF epithelia: implications for the role of CFTR in RANTES expression Am J Physiol Cell Physiol, March 1, 1999; 276(3): C700 - C710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. S. Xu, C. Vanderziel, C. F. Bennett, and B. P. Monia A Role for c-Raf Kinase and Ha-Ras in Cytokine-mediated Induction of Cell Adhesion Molecules J. Biol. Chem., December 11, 1998; 273(50): 33230 - 33238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Lazaar, Y. Amrani, J. Hsu, R. A. Panettieri Jr., W. C. Fanslow, S. M. Albelda, and E. Pure CD40-Mediated Signal Transduction in Human Airway Smooth Muscle J. Immunol., September 15, 1998; 161(6): 3120 - 3127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Miskin, C. C. Abrams, L. C. Goatley, and L. K. Dixon A Viral Mechanism for Inhibition of the Cellular Phosphatase Calcineurin Science, July 24, 1998; 281(5376): 562 - 565. [Abstract] [Full Text]
|
|
|
|
|
|
A. Sica, L. Dorman, V. Viggiano, M. Cippitelli, P. Ghosh, N. Rice, and H. A. Young Interaction of NF-kappa B and NFAT with the Interferon-gamma Promoter J. Biol. Chem., November 28, 1997; 272(48): 30412 - 30420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Nagata, E. Suzuki, H. Nishimatsu, H. Satonaka, A. Goto, M. Omata, and Y. Hirata Transcriptional Activation of the cyclin D1 Gene Is Mediated by Multiple Cis-Elements, Including SP1 Sites and a cAMP-responsive Element in Vascular Endothelial Cells J. Biol. Chem., January 5, 2001; 276(1): 662 - 669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Murat, C. Pellieux, H.-R. Brunner, and T. Pedrazzini Calcineurin Blockade Prevents Cardiac Mitogen-activated Protein Kinase Activation and Hypertrophy in Renovascular Hypertension J. Biol. Chem., December 22, 2000; 275(52): 40867 - 40873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Petranka, G. Wright, R. A. Forbes, and E. Murphy Elevated Calcium in Preneoplastic Cells Activates NF-kappa B and Confers Resistance to Apoptosis J. Biol. Chem., September 28, 2001; 276(40): 37102 - 37108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. W. Glazner, S. Camandola, J. D. Geiger, and M. P. Mattson Endoplasmic Reticulum D-myo-Inositol 1,4,5-Trisphosphate-sensitive Stores Regulate Nuclear Factor-kappa B Binding Activity in a Calcium-independent Manner J. Biol. Chem., June 15, 2001; 276(25): 22461 - 22467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Millet, R. J. Phillips, R. S. Sherwin, S. Ghosh, R. E. Voll, R. A. Flavell, A. Vignery, and M. Rincon Inhibition of NF-kappa B Activity and Enhancement of Apoptosis by the Neuropeptide Calcitonin Gene-related Peptide J. Biol. Chem., May 12, 2000; 275(20): 15114 - 15121. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
