The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peola, S.
Right arrow Articles by Massaia, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peola, S.
Right arrow Articles by Massaia, M.

The Journal of Immunology, Vol 157, Issue 10 4354-4362, Copyright © 1996 by American Association of Immunologists

ARTICLES

Selective induction of CD73 expression in human lymphocytes by CD38 ligation: a novel pathway linking signal transducers with ecto-enzyme activities

S Peola, P Borrione, L Matera, F Malavasi, A Pileri and M Massaia
Division of Hematology of the University of Torino, Italy.

CD73 is a glycosyl phosphatidylinositol (GPI)-anchored purine salvage enzyme (ecto-5'-nucleotidase (ecto-5'-NT), E.C. 3.1.3.5) whose expression on lymphocytes is dependent on their differentiation state and function. CD73 behaves as an agonistic molecule in signaling via the CD3/TCR and CD2 pathways and is associated with CTL generation, IgG production, and activation of resting naive CD8+ T cells. CD73 deficiency has been reported in a variety of patients with impaired T and/or B cell function. Thus, CD73 holds promise as a molecular target for intervention in the immune system, but the mechanisms regulating its expression are largely unknown. The aim of this study was to gain insight into the regulation of CD73 expression in human lymphocytes. CD38, another cell surface differentiation Ag with ecto-enzyme activities (NAD+ glycohydrolase, ADP-ribosyl cyclase, and cyclic ADP- ribose (cADPR) hydrolase), was found to specifically induce CD73 expression in T and B cell lines as well as in normal adult T and NK cells, cord blood T cells, and thymocytes. CD38 cross-linking induced a rapid export to the cell surface of pre-formed CD73 derived from an intracellular pool and not from de novo biosynthesis. This translocation was dependent on protein tyrosine kinase (PTK) activity and lasted approximately eight hours, after which CD73 was removed from the cell surface by enzymatic cleavage. CD73 was not induced by other agents that activate T cells and CD73 was the only GPI-anchored molecule up-regulated by CD38 ligation out of six analyzed. These results document a novel pathway in human lymphocytes leading from CD38 ligation to CD73 expression, which may result in the rapid acquisition of new functions, including increased purine salvage, increased sensitivity to Ag-induced activation, and the generation of adenosine (Ado) for Ado receptor signaling.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
J. Niemela, T. Henttinen, G. G. Yegutkin, L. Airas, A.-M. Kujari, P. Rajala, and S. Jalkanen
IFN-{alpha} Induced Adenosine Production on the Endothelium: A Mechanism Mediated by CD73 (Ecto-5'-Nucleotidase) Up-Regulation
J. Immunol., February 1, 2004; 172(3): 1646 - 1653.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Majumdar and B. B. Aggarwal
Methotrexate Suppresses NF-{kappa}B Activation Through Inhibition of I{kappa}B{alpha} Phosphorylation and Degradation
J. Immunol., September 1, 2001; 167(5): 2911 - 2920.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Bortell, J. Moss, R. C. McKenna, M. R. Rigby, D. Niedzwiecki, L. A. Stevens, W. A. Patton, J. P. Mordes, D. L. Greiner, and A. A. Rossini
Nicotinamide Adenine Dinucleotide (NAD) and Its Metabolites Inhibit T Lymphocyte Proliferation: Role of Cell Surface NAD Glycohydrolase and Pyrophosphatase Activities
J. Immunol., August 15, 2001; 167(4): 2049 - 2059.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. Sconocchia, J. A. Titus, A. Mazzoni, A. Visintin, F. Pericle, S. W. Hicks, F. Malavasi, and D. M. Segal
CD38 Triggers Cytotoxic Responses in Activated Human Natural Killer Cells
Blood, December 1, 1999; 94(11): 3864 - 3871.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Borrione, S. Peola, S. Mariani, B. Besostri, R. Mallone, F. Malavasi, A. Pileri, and M. Massaia
CD38 Stimulation Lowers the Activation Threshold and Enhances the Alloreactivity of Cord Blood T Cells by Activating the Phosphatidylinositol 3-Kinase Pathway and Inducing CD73 Expression
J. Immunol., May 15, 1999; 162(10): 6238 - 6246.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Deaglio, M. Morra, R. Mallone, C. M. Ausiello, E. Prager, G. Garbarino, U. Dianzani, H. Stockinger, and F. Malavasi
Human CD38 (ADP-Ribosyl Cyclase) Is a Counter-Receptor of CD31, an Ig Superfamily Member
J. Immunol., January 1, 1998; 160(1): 395 - 402.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
J. Corbeil, D. Sheeter, D. Genini, S. Rought, L. Leoni, P. Du, M. Ferguson, D. R. Masys, J. B. Welsh, J. L. Fink, et al.
Temporal Gene Regulation During HIV-1 Infection of Human CD4+ T Cells
Genome Res., July 1, 2001; 11(7): 1198 - 1204.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.