The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wolbink, G. J.
Right arrow Articles by Hack, C. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wolbink, G. J.
Right arrow Articles by Hack, C. E.

The Journal of Immunology, Vol 157, Issue 1 473-479, Copyright © 1996 by American Association of Immunologists

ARTICLES

CRP-mediated activation of complement in vivo: assessment by measuring circulating complement-C-reactive protein complexes

GJ Wolbink, MC Brouwer, S Buysmann, IJ ten Berge and CE Hack
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.

The in vivo function of C-reactive protein (CRP) is unknown. Among the in vitro functions assigned to CRP is the ability to activate complement via the classical pathway. To date, there is no evidence supporting that CRP exerts this function in vivo. We here show a novel approach to assess CRP-mediated complement activation in vivo, which is based on the property that activated complement factors C3 and C4 fix to CRP during complement activation induced by this acute phase protein. We developed specific ELISAs for complexes between CRP and C4b, C4d, C3b, or C3d. We established that in vitro complement-CRP complexes were formed only during CRP-dependent activation, and not during activation by other activators, even in the presence of high CRP levels. Circulating levels of complement-CRP complexes were undetectable in normal donors, but significantly increased in nine patients following implantation of a renal allograft. Importantly, levels of complement-CRP complexes did not change in these patients upon a bolus infusion of mAb OKT3, which induces activation of the classical complement pathway, demonstrating in vivo that complement-CRP complexes are not formed during CRP-independent activation of complement, even when CRP is elevated. We conclude that measurement of complement-CRP complexes provides a suitable tool to study CRP-mediated activation of complement in vivo. Furthermore, increased levels of these complexes occur in clinical samples, indicating that CRP may induce activation of complement in vivo.


This article has been cited by other articles:


Home page
 Clin. Chem.Home page
J. Hartweg, M. Gunter, R. Perera, A. Farmer, C. Cull, C. Schalkwijk, A. Kok, H. Twaalfhoven, R. Holman, and A. Neil
Stability of Soluble Adhesion Molecules, Selectins, and C-Reactive Protein at Various Temperatures: Implications for Epidemiological and Large-Scale Clinical Studies
Clin. Chem., October 1, 2007; 53(10): 1858 - 1860.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
E. Biro, R. Nieuwland, P. P Tak, L. M Pronk, M. C L Schaap, A. Sturk, and C E. Hack
Activated complement components and complement activator molecules on the surface of cell-derived microparticles in patients with rheumatoid arthritis and healthy individuals
Ann Rheum Dis, August 1, 2007; 66(8): 1085 - 1092.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
F. Blaschke, Y. Takata, E. Caglayan, A. Collins, P. Tontonoz, W. A. Hsueh, and R. K. Tangirala
A Nuclear Receptor Corepressor-Dependent Pathway Mediates Suppression of Cytokine-Induced C-Reactive Protein Gene Expression by Liver X Receptor
Circ. Res., December 8, 2006; 99(12): e88 - e99.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. P. Sjoberg, L. A. Trouw, F. D. G. McGrath, C. E. Hack, and A. M. Blom
Regulation of complement activation by C-reactive protein: targeting of the inhibitory activity of c4b-binding protein.
J. Immunol., June 15, 2006; 176(12): 7612 - 7620.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
A. M. Draaisma, M. G. Hazekamp, N. Anes, P. H. Schoof, C. E. Hack, A. Sturk, and R. A.E. Dion
Phosphorylcholine Coating of Bypass Systems Used for Young Infants Does Not Attenuate the Inflammatory Response
Ann. Thorac. Surg., April 1, 2006; 81(4): 1455 - 1459.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
M Meuwissen, A C van der Wal, H W M Niessen, K T Koch, R J de Winter, C M van der Loos, S Z H Rittersma, S A J Chamuleau, J G P Tijssen, A E Becker, et al.
Colocalisation of intraplaque C reactive protein, complement, oxidised low density lipoprotein, and macrophages in stable and unstable angina and acute myocardial infarction
J. Clin. Pathol., February 1, 2006; 59(2): 196 - 201.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
S. B. Schwedler, K. Amann, K. Wernicke, A. Krebs, M. Nauck, C. Wanner, L. A. Potempa, and J. Galle
Native C-Reactive Protein Increases Whereas Modified C-Reactive Protein Reduces Atherosclerosis in Apolipoprotein E-Knockout Mice
Circulation, August 16, 2005; 112(7): 1016 - 1023.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
T. Inoue, T. Kato, T. Uchida, M. Sakuma, A. Nakajima, M. Shibazaki, Y. Imoto, M. Saito, S. Hashimoto, Y. Hikichi, et al.
Local Release of C-Reactive Protein From Vulnerable Plaque or Coronary Arterial Wall Injured by Stenting
J. Am. Coll. Cardiol., July 19, 2005; 46(2): 239 - 245.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
A Familian, A E Voskuyl, G J van Mierlo, H A Heijst, J W R Twisk, B A C Dijkmans, and C E Hack
Infliximab treatment reduces complement activation in patients with rheumatoid arthritis
Ann Rheum Dis, July 1, 2005; 64(7): 1003 - 1008.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. Cunningham, D. C. Wilcockson, D. Boche, and V. H. Perry
Comparison of Inflammatory and Acute-Phase Responses in the Brain and Peripheral Organs of the ME7 Model of Prion Disease
J. Virol., April 15, 2005; 79(8): 5174 - 5184.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
D. A. Lauver, E. A. Booth, A. J. White, E. Poradosu, and B. R. Lucchesi
Sulodexide Attenuates Myocardial Ischemia/Reperfusion Injury and the Deposition of C-Reactive Protein in Areas of Infarction without Affecting Hemostasis
J. Pharmacol. Exp. Ther., February 1, 2005; 312(2): 794 - 800.
[Abstract] [Full Text] [PDF]

Home page
 HeartHome page
T Sampietro, F Bigazzi, B D. Pino, G Rossi, E Chella, S Lusso, M Puntoni, M Tuoni, and A Bionda
Up regulation of C3, C4, and soluble intercellular adhesion molecule-1 co-expresses with high sensitivity C reactive protein in familial hypoalphalipoproteinaemia: further evidence of inflammatory activation
Heart, December 1, 2004; 90(12): 1438 - 1442.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
F. Blaschke, D. Bruemmer, F. Yin, Y. Takata, W. Wang, M. C. Fishbein, T. Okura, J. Higaki, K. Graf, E. Fleck, et al.
C-Reactive Protein Induces Apoptosis in Human Coronary Vascular Smooth Muscle Cells
Circulation, August 3, 2004; 110(5): 579 - 587.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
R. de Vroege, W. van Oeveren, J. van Klarenbosch, W. Stooker, M. A. J. M. Huybregts, C. E. Hack, L. van Barneveld, L. Eijsman, and C. R. H. Wildevuur
The Impact of Heparin-Coated Cardiopulmonary Bypass Circuits on Pulmonary Function and the Release of Inflammatory Mediators
Anesth. Analg., June 1, 2004; 98(6): 1586 - 1594.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. T. Lane
Microalbuminuria as a marker of cardiovascular and renal risk in type 2 diabetes mellitus: a temporal perspective
Am J Physiol Renal Physiol, March 1, 2004; 286(3): F442 - F450.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
S.-H. Li, P. E. Szmitko, R. D. Weisel, C.-H. Wang, P. W.M. Fedak, R.-K. Li, D. A.G. Mickle, and S. Verma
C-Reactive Protein Upregulates Complement-Inhibitory Factors in Endothelial Cells
Circulation, February 24, 2004; 109(7): 833 - 836.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Nijmeijer, M. Willemsen, C. J. L. M. Meijer, C. A. Visser, R. H. Verheijen, R. A. Gottlieb, C. E. Hack, and H. W. M. Niessen
Type II secretory phospholipase A2 binds to ischemic flip-flopped cardiomyocytes and subsequently induces cell death
Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H2218 - H2224.
[Abstract] [Full Text] [PDF]

Home page
 PediatricsHome page
T. W. Kuijpers, M. Biezeveld, A. Achterhuis, I. Kuipers, J. Lam, C. E. Hack, A. E. Becker, and A. C. van der Wal
Longstanding Obliterative Panarteritis in Kawasaki Disease: Lack of Cyclosporin A Effect
Pediatrics, October 1, 2003; 112(4): 986 - 992.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
R. Nijmeijer, W. K. Lagrand, Y. T. P. Lubbers, C. A. Visser, C. J. L. M. Meijer, H. W. M. Niessen, and C. E. Hack
C-Reactive Protein Activates Complement in Infarcted Human Myocardium
Am. J. Pathol., July 1, 2003; 163(1): 269 - 275.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
A. Silvestri, O. Gebara, C. Vitale, M. Wajngarten, F. Leonardo, J. A.F. Ramires, M. Fini, G. Mercuro, and G. M.C. Rosano
Increased Levels of C-Reactive Protein After Oral Hormone Replacement Therapy May Not Be Related to an Increased Inflammatory Response
Circulation, July 1, 2003; 107(25): 3165 - 3169.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
V. Stangl, G. Baumann, and K. Stangl
Coronary atherogenic risk factors in women
Eur. Heart J., November 2, 2002; 23(22): 1738 - 1752.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
D. C. Chan, G. F. Watts, P. H. R. Barrett, L. J. Beilin, and T. A. Mori
Effect of Atorvastatin and Fish Oil on Plasma High-Sensitivity C-Reactive Protein Concentrations in Individuals with Visceral Obesity
Clin. Chem., June 1, 2002; 48(6): 877 - 883.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
I. Glurich, S. Grossi, B. Albini, A. Ho, R. Shah, M. Zeid, H. Baumann, R. J. Genco, and E. De Nardin
Systemic Inflammation in Cardiovascular and Periodontal Disease: Comparative Study
Clin. Vaccine Immunol., March 1, 2002; 9(2): 425 - 432.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
R. Nijmeijer, W. K Lagrand, A. Baidoshvili, Y. T.P Lubbers, W. T. Hermens, C. J.L.M Meijer, C. A Visser, C.E. Hack, and H. W.M Niessen
Secretory type II phospholipase A2 binds to ischemic myocardium during myocardial infarction in humans
Cardiovasc Res, January 1, 2002; 53(1): 138 - 146.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
K. Yasojima, C. Schwab, E. G. McGeer, and P. L. McGeer
Complement Components, but Not Complement Inhibitors, Are Upregulated in Atherosclerotic Plaques
Arterioscler. Thromb. Vasc. Biol., July 1, 2001; 21(7): 1214 - 1219.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
K. Yasojima, C. Schwab, E. G. McGeer, and P. L. McGeer
Generation of C-Reactive Protein and Complement Components in Atherosclerotic Plaques
Am. J. Pathol., March 1, 2001; 158(3): 1039 - 1051.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
M. S. Eisenberg, H. J. Chen, M. K. Warshofsky, R. R. Sciacca, H. S. Wasserman, A. Schwartz, and L. E. Rabbani
Elevated Levels of Plasma C-Reactive Protein Are Associated With Decreased Graft Survival in Cardiac Transplant Recipients
Circulation, October 24, 2000; 102(17): 2100 - 2104.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
W. K. Lagrand, C. A. Visser, C. E. Hack, H. W. M. Niessen, R. Nijmeijer, W. Konig, and C. Wanner
C-reactive protein and cardiovascular disease: linked by complement?
Nephrol. Dial. Transplant., October 1, 2000; 15(10): 1709 - 1710.
[Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Torzewski, C. Rist, R. F. Mortensen, T. P. Zwaka, M. Bienek, J. Waltenberger, W. Koenig, G. Schmitz, V. Hombach, and J. Torzewski
C-Reactive Protein in the Arterial Intima : Role of C-Reactive Protein Receptor-Dependent Monocyte Recruitment in Atherogenesis
Arterioscler. Thromb. Vasc. Biol., September 1, 2000; 20(9): 2094 - 2099.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
A. Festa, R. D'Agostino Jr, G. Howard, L. Mykkanen, R. P. Tracy, and S. M. Haffner
Chronic Subclinical Inflammation as Part of the Insulin Resistance Syndrome : The Insulin Resistance Atherosclerosis Study (IRAS)
Circulation, July 4, 2000; 102(1): 42 - 47.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
M.-C Tataru, J Heinrich, R Junker, H Schulte, A von Eckardstein, G Assmann, and E Koehler
C-reactive protein and the severity of atherosclerosis in myocardial infarction patients with stable angina pectoris
Eur. Heart J., June 2, 2000; 21(12): 1000 - 1008.
[Abstract] [PDF]

Home page
 Pharmacol. Rev.Home page
C. Caliezi, W. A. Wuillemin, S. Zeerleder, M. Redondo, B. Eisele, and C. E. Hack
C1-Esterase Inhibitor: An Anti-Inflammatory Agent and Its Potential Use in the Treatment of Diseases Other Than Hereditary Angioedema
Pharmacol. Rev., March 1, 2000; 52(1): 91 - 112.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
B. W. Walsh, S. Paul, R. A. Wild, R. A. Dean, R. P. Tracy, D. A. Cox, and P. W. Anderson
The Effects of Hormone Replacement Therapy and Raloxifene on C-Reactive Protein and Homocysteine in Healthy Postmenopausal Women: A Randomized, Controlled Trial
J. Clin. Endocrinol. Metab., January 1, 2000; 85(1): 214 - 218.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S. Bhakdi, M. Torzewski, M. Klouche, and M. Hemmes
Complement and Atherogenesis : Binding of CRP to Degraded, Nonoxidized LDL Enhances Complement Activation
Arterioscler. Thromb. Vasc. Biol., October 1, 1999; 19(10): 2348 - 2354.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Jarva, T. S. Jokiranta, J. Hellwage, P. F. Zipfel, and S. Meri
Regulation of Complement Activation by C-Reactive Protein: Targeting the Complement Inhibitory Activity of Factor H by an Interaction with Short Consensus Repeat Domains 7 and 8-11
J. Immunol., October 1, 1999; 163(7): 3957 - 3962.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
W. K. Lagrand, C. A. Visser, W. T. Hermens, H. W. M. Niessen, F. W. A. Verheugt, G.-J. Wolbink, and C. E. Hack
C-Reactive Protein as a Cardiovascular Risk Factor : More Than an Epiphenomenon?
Circulation, July 6, 1999; 100(1): 96 - 102.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. A. Hazelzet, R. de Groot, G. van Mierlo, K. F. M. Joosten, E. van der Voort, A. Eerenberg, M. H. Suur, W. C. J. Hop, and C. E. Hack
Complement Activation in Relation to Capillary Leakage in Children with Septic Shock and Purpura
Infect. Immun., November 1, 1998; 66(11): 5350 - 5356.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. Torzewski, M. Torzewski, D. E. Bowyer, M. Frohlich, W. Koenig, J. Waltenberger, C. Fitzsimmons, and V. Hombach
C-Reactive Protein Frequently Colocalizes With the Terminal Complement Complex in the Intima of Early Atherosclerotic Lesions of Human Coronary Arteries
Arterioscler. Thromb. Vasc. Biol., September 1, 1998; 18(9): 1386 - 1392.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
P. M. Ridker, M. Cushman, M. J. Stampfer, R. P. Tracy, and C. H. Hennekens
Plasma Concentration of C-Reactive Protein and Risk of Developing Peripheral Vascular Disease
Circulation, February 10, 1998; 97(5): 425 - 428.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
P. Bruins, H. t. Velthuis, A. P. Yazdanbakhsh, P. G. M. Jansen, F. W. J. van Hardevelt, E. M. F. H. de Beaumont, C. R. H. Wildevuur, L. Eijsman, A. Trouwborst, and C. E. Hack
Activation of the Complement System During and After Cardiopulmonary Bypass Surgery : Postsurgery Activation Involves C-Reactive Protein and Is Associated With Postoperative Arrhythmia
Circulation, November 18, 1997; 96(10): 3542 - 3548.
[Abstract] [Full Text]

Home page
 CirculationHome page
W. K. Lagrand, H. W.M. Niessen, G.-J. Wolbink, L. H. Jaspars, C. A. Visser, F. W.A. Verheugt, C. J.L.M. Meijer, and C. E. Hack
C-Reactive Protein Colocalizes With Complement in Human Hearts During Acute Myocardial Infarction
Circulation, January 7, 1997; 95(1): 97 - 103.
[Abstract] [Full Text]

Home page
 CirculationHome page
A. Wakatsuki, Y. Okatani, N. Ikenoue, and T. Fukaya
Effect of Medroxyprogesterone Acetate on Vascular Inflammatory Markers in Postmenopausal Women Receiving Estrogen
Circulation, March 26, 2002; 105(12): 1436 - 1439.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1996 by The American Association of Immunologists, Inc. All rights reserved.